ID   CIAO1_RAT               Reviewed;         339 AA.
AC   Q5M7T1;
DT   20-MAR-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-2005, sequence version 1.
DT   03-AUG-2022, entry version 115.
DE   RecName: Full=Probable cytosolic iron-sulfur protein assembly protein CIAO1 {ECO:0000255|HAMAP-Rule:MF_03037};
DE   AltName: Full=WD repeat-containing protein 39 {ECO:0000255|HAMAP-Rule:MF_03037};
GN   Name=Ciao1 {ECO:0000255|HAMAP-Rule:MF_03037}; Synonyms=Wdr39;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Kidney;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- FUNCTION: Key component of the cytosolic iron-sulfur protein assembly
CC       (CIA) complex, a multiprotein complex that mediates the incorporation
CC       of iron-sulfur cluster into extramitochondrial Fe/S proteins (By
CC       similarity). As a CIA complex component, interacts specifically with
CC       CIAO2A or CIAO2B and MMS19 to assist different branches of iron-sulfur
CC       protein assembly, depending of its interactors. The complex
CC       CIAO1:CIAO2B:MMS19 binds to and facilitates the assembly of most
CC       cytosolic-nuclear Fe/S proteins. CIAO1:CIAO2A specifically matures ACO1
CC       and stabilizes IREB2 (By similarity). Seems to specifically modulate
CC       the transactivation activity of WT1. As part of the mitotic spindle-
CC       associated MMXD complex it may play a role in chromosome segregation
CC       (By similarity). {ECO:0000250|UniProtKB:O76071, ECO:0000255|HAMAP-
CC       Rule:MF_03037}.
CC   -!- SUBUNIT: Component of the CIA complex. Interacts with CIAO2A and forms
CC       a complex with CIAO2B and MMS19; the interactions with CIAO2A and
CC       CIAO2B are mutually exclusive. Interacts with CHD1L, ERCC2, IREB2 and
CC       POLD1. Component of the MMXD complex, which includes CIAO1, ERCC2,
CC       CIAO2B, MMS19 and SLC25A5. Interacts with WT1. Interacts with CIAO3.
CC       Interacts (via LYR motif) with HSC20. {ECO:0000250|UniProtKB:O76071,
CC       ECO:0000255|HAMAP-Rule:MF_03037}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:O76071}.
CC   -!- SIMILARITY: Belongs to the WD repeat CIA1 family. {ECO:0000255|HAMAP-
CC       Rule:MF_03037}.
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DR   EMBL; BC088474; AAH88474.1; -; mRNA.
DR   RefSeq; NP_001008766.1; NM_001008766.1.
DR   AlphaFoldDB; Q5M7T1; -.
DR   SMR; Q5M7T1; -.
DR   STRING; 10116.ENSRNOP00000017603; -.
DR   PhosphoSitePlus; Q5M7T1; -.
DR   jPOST; Q5M7T1; -.
DR   PaxDb; Q5M7T1; -.
DR   PRIDE; Q5M7T1; -.
DR   GeneID; 29231; -.
DR   KEGG; rno:29231; -.
DR   UCSC; RGD:1307285; rat.
DR   CTD; 9391; -.
DR   RGD; 1307285; Ciao1.
DR   VEuPathDB; HostDB:ENSRNOG00000012638; -.
DR   eggNOG; KOG0645; Eukaryota.
DR   InParanoid; Q5M7T1; -.
DR   OMA; AIWSKSS; -.
DR   OrthoDB; 793298at2759; -.
DR   PhylomeDB; Q5M7T1; -.
DR   TreeFam; TF318181; -.
DR   PRO; PR:Q5M7T1; -.
DR   Proteomes; UP000002494; Chromosome 3.
DR   Bgee; ENSRNOG00000012638; Expressed in pancreas and 19 other tissues.
DR   ExpressionAtlas; Q5M7T1; baseline and differential.
DR   Genevisible; Q5M7T1; RN.
DR   GO; GO:0097361; C:CIA complex; ISS:UniProtKB.
DR   GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR   GO; GO:0071817; C:MMXD complex; ISS:UniProtKB.
DR   GO; GO:0007059; P:chromosome segregation; IEA:UniProtKB-KW.
DR   GO; GO:0016226; P:iron-sulfur cluster assembly; ISS:UniProtKB.
DR   GO; GO:0097428; P:protein maturation by iron-sulfur cluster transfer; ISS:UniProtKB.
DR   Gene3D; 2.130.10.10; -; 1.
DR   HAMAP; MF_03037; ciao1; 1.
DR   InterPro; IPR028608; CIAO1/Cia1.
DR   InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR   InterPro; IPR001680; WD40_repeat.
DR   InterPro; IPR019775; WD40_repeat_CS.
DR   InterPro; IPR036322; WD40_repeat_dom_sf.
DR   PANTHER; PTHR19920; PTHR19920; 1.
DR   Pfam; PF00400; WD40; 6.
DR   SMART; SM00320; WD40; 7.
DR   SUPFAM; SSF50978; SSF50978; 1.
DR   PROSITE; PS00678; WD_REPEATS_1; 1.
DR   PROSITE; PS50082; WD_REPEATS_2; 6.
DR   PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE   2: Evidence at transcript level;
KW   Chromosome partition; Cytoplasm; Reference proteome; Repeat; WD repeat.
FT   CHAIN           1..339
FT                   /note="Probable cytosolic iron-sulfur protein assembly
FT                   protein CIAO1"
FT                   /id="PRO_0000281107"
FT   REPEAT          14..53
FT                   /note="WD 1"
FT   REPEAT          59..98
FT                   /note="WD 2"
FT   REPEAT          103..142
FT                   /note="WD 3"
FT   REPEAT          148..187
FT                   /note="WD 4"
FT   REPEAT          192..231
FT                   /note="WD 5"
FT   REPEAT          250..289
FT                   /note="WD 6"
FT   REPEAT          301..339
FT                   /note="WD 7"
FT   MOTIF           176..178
FT                   /note="LYR motif; required for interaction with HSC20"
FT                   /evidence="ECO:0000250|UniProtKB:O76071"
SQ   SEQUENCE   339 AA;  37618 MW;  6B257EB84356C0F8 CRC64;
     MKDALVLQSR VPAHPDSRCW FLAWNPTGTL LASCGGDRKI RIWGTEGDSW ICKSVLSEGH
     QRTVRKVAWS PCGNYLASAS FDATTCIWKK NQDDFECVTT LEGHENEVKS VAWAPSGNLL
     ATCSRDKSVW VWEVDEEDEY ECVSVLNSHT QDVKHVVWHP SQELLASASY DDTVKLYQEE
     GDDWVCCATL EGHESTVWSI AFDPSGQRLA SCSDDRTVRI WRQYLPGNEQ GVACSGSDPS
     WKCVCTLSGF HTRTIYDVAW CQLTGALATA CGDDAIRVFE EDPGSDPQQP TFSLTAHLRQ
     AHSQDVNCVA WNPKEAGLLA SCSDDGEVAF WEYHQPAGL