ID   CIAR_STRR6              Reviewed;         224 AA.
AC   P0A4H8; Q54954;
DT   15-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT   15-MAR-2005, sequence version 1.
DT   25-MAY-2022, entry version 103.
DE   RecName: Full=Transcriptional regulatory protein CiaR;
GN   Name=ciaR; OrderedLocusNames=spr0707;
OS   Streptococcus pneumoniae (strain ATCC BAA-255 / R6).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC   Streptococcus.
OX   NCBI_TaxID=171101;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=8065267; DOI=10.1111/j.1365-2958.1994.tb01038.x;
RA   Guenzi E., Gasc A.M., Sicard M.A., Hakenbeck R.;
RT   "A two-component signal-transducing system is involved in competence and
RT   penicillin susceptibility in laboratory mutants of Streptococcus
RT   pneumoniae.";
RL   Mol. Microbiol. 12:505-515(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-255 / R6;
RX   PubMed=11544234; DOI=10.1128/jb.183.19.5709-5717.2001;
RA   Hoskins J., Alborn W.E. Jr., Arnold J., Blaszczak L.C., Burgett S.,
RA   DeHoff B.S., Estrem S.T., Fritz L., Fu D.-J., Fuller W., Geringer C.,
RA   Gilmour R., Glass J.S., Khoja H., Kraft A.R., Lagace R.E., LeBlanc D.J.,
RA   Lee L.N., Lefkowitz E.J., Lu J., Matsushima P., McAhren S.M., McHenney M.,
RA   McLeaster K., Mundy C.W., Nicas T.I., Norris F.H., O'Gara M., Peery R.B.,
RA   Robertson G.T., Rockey P., Sun P.-M., Winkler M.E., Yang Y.,
RA   Young-Bellido M., Zhao G., Zook C.A., Baltz R.H., Jaskunas S.R.,
RA   Rosteck P.R. Jr., Skatrud P.L., Glass J.I.;
RT   "Genome of the bacterium Streptococcus pneumoniae strain R6.";
RL   J. Bacteriol. 183:5709-5717(2001).
CC   -!- FUNCTION: Member of the two-component regulatory system CiaH/CiaR.
CC       Involved in early steps of competence regulation and in penicillin
CC       susceptibility.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC   -!- PTM: Phosphorylated by CiaH. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAK99511.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; X77249; CAA54465.1; -; Genomic_DNA.
DR   EMBL; AE007317; AAK99511.1; ALT_INIT; Genomic_DNA.
DR   PIR; S49544; S49544.
DR   RefSeq; NP_358301.1; NC_003098.1.
DR   RefSeq; WP_000590640.1; NC_003098.1.
DR   AlphaFoldDB; P0A4H8; -.
DR   SMR; P0A4H8; -.
DR   STRING; 171101.spr0707; -.
DR   EnsemblBacteria; AAK99511; AAK99511; spr0707.
DR   GeneID; 60232948; -.
DR   GeneID; 66805944; -.
DR   KEGG; spr:spr0707; -.
DR   PATRIC; fig|171101.6.peg.783; -.
DR   eggNOG; COG0745; Bacteria.
DR   HOGENOM; CLU_000445_30_1_9; -.
DR   Proteomes; UP000000586; Chromosome.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0032993; C:protein-DNA complex; IBA:GO_Central.
DR   GO; GO:0001216; F:DNA-binding transcription activator activity; IBA:GO_Central.
DR   GO; GO:0000156; F:phosphorelay response regulator activity; IBA:GO_Central.
DR   GO; GO:0000976; F:transcription cis-regulatory region binding; IBA:GO_Central.
DR   GO; GO:0030420; P:establishment of competence for transformation; IEA:UniProtKB-KW.
DR   CDD; cd00383; trans_reg_C; 1.
DR   Gene3D; 1.10.10.10; -; 1.
DR   InterPro; IPR011006; CheY-like_superfamily.
DR   InterPro; IPR001867; OmpR/PhoB-type_DNA-bd.
DR   InterPro; IPR001789; Sig_transdc_resp-reg_receiver.
DR   InterPro; IPR039420; WalR-like.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   PANTHER; PTHR48111; PTHR48111; 1.
DR   Pfam; PF00072; Response_reg; 1.
DR   Pfam; PF00486; Trans_reg_C; 1.
DR   SMART; SM00448; REC; 1.
DR   SMART; SM00862; Trans_reg_C; 1.
DR   SUPFAM; SSF52172; SSF52172; 1.
DR   PROSITE; PS51755; OMPR_PHOB; 1.
DR   PROSITE; PS50110; RESPONSE_REGULATORY; 1.
PE   3: Inferred from homology;
KW   Competence; Cytoplasm; DNA-binding; Phosphoprotein; Reference proteome;
KW   Transcription; Transcription regulation; Two-component regulatory system.
FT   CHAIN           1..224
FT                   /note="Transcriptional regulatory protein CiaR"
FT                   /id="PRO_0000081062"
FT   DOMAIN          3..116
FT                   /note="Response regulatory"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
FT   DNA_BIND        124..222
FT                   /note="OmpR/PhoB-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01091"
FT   MOD_RES         51
FT                   /note="4-aspartylphosphate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
SQ   SEQUENCE   224 AA;  25466 MW;  1CE646532B2E3A8F CRC64;
     MIKILLVEDD LGLSNSVFDF LDDFADVMQV FDGEEGLYEA ESGVYDLILL DLMLPEKNGF
     QVLKELREKG ITTPVLIMTA KESLDDKGHG FELGADDYLT KPFYLEELKM RIQALLKRSG
     KFNENTLTYG NIVVNLSTNT VKVEDTPVEL LGKEFDLLVY FLQNQNVILP KTQIFDRLWG
     FDSDTTISVV EVYVSKVRKK LKGTTFAENL QTLRSVGYLL KDVQ