CIB2_RAT
ID CIB2_RAT Reviewed; 187 AA.
AC Q568Z7;
DT 29-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 10-MAY-2005, sequence version 1.
DT 03-AUG-2022, entry version 107.
DE RecName: Full=Calcium and integrin-binding family member 2;
GN Name=Cib2;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Calcium-binding protein critical for proper photoreceptor
CC cell maintenance and function. Plays a role in intracellular calcium
CC homeostasis by decreasing ATP-induced calcium release. May be involved
CC in the mechanotransduction process. {ECO:0000250,
CC ECO:0000250|UniProtKB:O75838}.
CC -!- SUBUNIT: Homodimer. Interacts with WHRN and MYO7A. Interacts with
CC ITGA2B (via C-terminus cytoplasmic tail region) and ITGA7 (via C-
CC terminus cytoplasmic tail region); the interactions are
CC stabilized/increased in a calcium and magnesium-dependent manner.
CC {ECO:0000250|UniProtKB:O75838}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q9Z309}. Cell
CC projection, stereocilium {ECO:0000250|UniProtKB:O75838}. Photoreceptor
CC inner segment {ECO:0000250|UniProtKB:Q9Z309}. Cell projection, cilium,
CC photoreceptor outer segment {ECO:0000250|UniProtKB:Q9Z309}. Cell
CC membrane, sarcolemma {ECO:0000250|UniProtKB:Q9Z309}. Note=Colocalized
CC with ITGA7 at the myotendinous junctions (MTJ) and at the neuromuscular
CC junctions (NMJ). Localizes in the cuticular plate along and at the tip
CC of the stereocilia of vestibular sensory hair cells.
CC {ECO:0000250|UniProtKB:O75838, ECO:0000250|UniProtKB:Q9Z309}.
CC -!- MISCELLANEOUS: The binding of either calcium or magnesium significantly
CC increases the structural stability of the protein in comparison to apo-
CC CIB (calcium- and magnesium-free form). {ECO:0000250|UniProtKB:O75838}.
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DR EMBL; BC092630; AAH92630.1; -; mRNA.
DR RefSeq; NP_001015010.1; NM_001015010.1.
DR AlphaFoldDB; Q568Z7; -.
DR SMR; Q568Z7; -.
DR STRING; 10116.ENSRNOP00000013623; -.
DR PaxDb; Q568Z7; -.
DR GeneID; 300719; -.
DR KEGG; rno:300719; -.
DR UCSC; RGD:1308718; rat.
DR CTD; 10518; -.
DR RGD; 1308718; Cib2.
DR eggNOG; KOG0038; Eukaryota.
DR HOGENOM; CLU_061288_6_0_1; -.
DR InParanoid; Q568Z7; -.
DR OMA; RYYEMAP; -.
DR OrthoDB; 1271942at2759; -.
DR PhylomeDB; Q568Z7; -.
DR TreeFam; TF313865; -.
DR PRO; PR:Q568Z7; -.
DR Proteomes; UP000002494; Chromosome 8.
DR Bgee; ENSRNOG00000059834; Expressed in quadriceps femoris and 18 other tissues.
DR Genevisible; Q568Z7; RN.
DR GO; GO:0071944; C:cell periphery; IBA:GO_Central.
DR GO; GO:0032437; C:cuticular plate; ISS:UniProtKB.
DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR GO; GO:0005829; C:cytosol; TAS:Reactome.
DR GO; GO:0005927; C:muscle tendon junction; ISO:RGD.
DR GO; GO:0031594; C:neuromuscular junction; ISO:RGD.
DR GO; GO:0001917; C:photoreceptor inner segment; ISS:UniProtKB.
DR GO; GO:0001750; C:photoreceptor outer segment; ISS:UniProtKB.
DR GO; GO:0042383; C:sarcolemma; ISO:RGD.
DR GO; GO:0032420; C:stereocilium; ISS:UniProtKB.
DR GO; GO:0005509; F:calcium ion binding; ISS:UniProtKB.
DR GO; GO:0005178; F:integrin binding; ISO:RGD.
DR GO; GO:0000287; F:magnesium ion binding; ISS:UniProtKB.
DR GO; GO:0042803; F:protein homodimerization activity; ISS:UniProtKB.
DR GO; GO:0044877; F:protein-containing complex binding; ISO:RGD.
DR GO; GO:0055074; P:calcium ion homeostasis; ISS:UniProtKB.
DR GO; GO:0071318; P:cellular response to ATP; ISS:UniProtKB.
DR GO; GO:0045494; P:photoreceptor cell maintenance; ISS:UniProtKB.
DR GO; GO:0007204; P:positive regulation of cytosolic calcium ion concentration; ISS:UniProtKB.
DR CDD; cd00051; EFh; 1.
DR InterPro; IPR011992; EF-hand-dom_pair.
DR InterPro; IPR018247; EF_Hand_1_Ca_BS.
DR InterPro; IPR002048; EF_hand_dom.
DR Pfam; PF13499; EF-hand_7; 1.
DR SMART; SM00054; EFh; 3.
DR SUPFAM; SSF47473; SSF47473; 1.
DR PROSITE; PS00018; EF_HAND_1; 2.
DR PROSITE; PS50222; EF_HAND_2; 3.
PE 2: Evidence at transcript level;
KW Calcium; Cell membrane; Cell projection; Cytoplasm; Magnesium; Membrane;
KW Metal-binding; Reference proteome; Repeat.
FT CHAIN 1..187
FT /note="Calcium and integrin-binding family member 2"
FT /id="PRO_0000289289"
FT DOMAIN 66..101
FT /note="EF-hand 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 103..138
FT /note="EF-hand 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 144..179
FT /note="EF-hand 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 116
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 118
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 120
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 127
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 157
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 159
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 161
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 163
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 168
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
SQ SEQUENCE 187 AA; 21672 MW; B97050DCBC0A6E0D CRC64;
MGNKQTIFTE EQLDNYQDCT FFNKKDILKL HARFYELAPN LVPMDYRKSP IVHVPMSLII
QMPELRENPF KERIVEAFSE DGEGNLTFND FVDMFSVLCE SAPRELKANY AFKIYDFNTD
NFICKEDLEL TLARLTKSEL DEDEVVLVCD KVIEEADLDG DGKLGFADFE DMIAKAPDFL
STFHIRI
