CIB3_HUMAN
ID CIB3_HUMAN Reviewed; 187 AA.
AC Q96Q77; E7EUX1; Q2M1W0; Q6ISP1;
DT 07-JUN-2004, integrated into UniProtKB/Swiss-Prot.
DT 18-MAY-2010, sequence version 3.
DT 03-AUG-2022, entry version 143.
DE RecName: Full=Calcium and integrin-binding family member 3;
DE AltName: Full=Kinase-interacting protein 3;
DE Short=KIP 3;
GN Name=CIB3; Synonyms=KIP3;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RA Hayashi A., Okaze H., Kozuma S., Saito T.;
RL Submitted (NOV-2000) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15057824; DOI=10.1038/nature02399;
RA Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E.,
RA Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A.,
RA Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S.,
RA Carrano A.V., Caoile C., Chan Y.M., Christensen M., Cleland C.A.,
RA Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J.,
RA Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M.,
RA Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W.,
RA Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V.,
RA Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D.,
RA McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I.,
RA Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L.,
RA Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A.,
RA She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M.,
RA Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J.,
RA Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E.,
RA Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M.,
RA Rubin E.M., Lucas S.M.;
RT "The DNA sequence and biology of human chromosome 19.";
RL Nature 428:529-535(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2), AND VARIANT
RP GLU-139.
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP INTERACTION WITH ITGA2B, MAGNESIUM-BINDING, AND CALCIUM-BINDING.
RX PubMed=22779914; DOI=10.1139/o2012-021;
RA Huang H., Bogstie J.N., Vogel H.J.;
RT "Biophysical and structural studies of the human calcium- and integrin-
RT binding protein family: understanding their functional similarities and
RT differences.";
RL Biochem. Cell Biol. 90:646-656(2012).
CC -!- SUBUNIT: Interacts with ITGA2B (via C-terminus cytoplasmic tail
CC region); the interaction is stabilized/increased in a calcium and
CC magnesium-dependent manner. {ECO:0000269|PubMed:22779914}.
CC -!- INTERACTION:
CC Q96Q77; Q8IUR7-6: ARMC8; NbExp=3; IntAct=EBI-10292696, EBI-11942961;
CC Q96Q77; Q8N7W2-2: BEND7; NbExp=3; IntAct=EBI-10292696, EBI-10181188;
CC Q96Q77; Q8WW18: C17orf50; NbExp=3; IntAct=EBI-10292696, EBI-12877892;
CC Q96Q77; Q16206-2: ENOX2; NbExp=3; IntAct=EBI-10292696, EBI-10179508;
CC Q96Q77; Q53SE7: FLJ13057; NbExp=3; IntAct=EBI-10292696, EBI-10172181;
CC Q96Q77; Q96IK5: GMCL1; NbExp=3; IntAct=EBI-10292696, EBI-2548508;
CC Q96Q77; Q8N6L0: KASH5; NbExp=3; IntAct=EBI-10292696, EBI-749265;
CC Q96Q77; Q5JT82: KLF17; NbExp=6; IntAct=EBI-10292696, EBI-10292791;
CC Q96Q77; Q8TBB1: LNX1; NbExp=3; IntAct=EBI-10292696, EBI-739832;
CC Q96Q77; P50221: MEOX1; NbExp=3; IntAct=EBI-10292696, EBI-2864512;
CC Q96Q77; P50222: MEOX2; NbExp=3; IntAct=EBI-10292696, EBI-748397;
CC Q96Q77; Q6FHY5: MEOX2; NbExp=3; IntAct=EBI-10292696, EBI-16439278;
CC Q96Q77; P60323-2: NANOS3; NbExp=3; IntAct=EBI-10292696, EBI-18012223;
CC Q96Q77; O00746: NME4; NbExp=3; IntAct=EBI-10292696, EBI-744871;
CC Q96Q77; Q02548: PAX5; NbExp=3; IntAct=EBI-10292696, EBI-296331;
CC Q96Q77; P26367: PAX6; NbExp=3; IntAct=EBI-10292696, EBI-747278;
CC Q96Q77; P62487: POLR2G; NbExp=3; IntAct=EBI-10292696, EBI-347928;
CC Q96Q77; P78424: POU6F2; NbExp=3; IntAct=EBI-10292696, EBI-12029004;
CC Q96Q77; Q9NYW8: RBAK; NbExp=3; IntAct=EBI-10292696, EBI-1210429;
CC Q96Q77; Q04864: REL; NbExp=3; IntAct=EBI-10292696, EBI-307352;
CC Q96Q77; Q9BWG6: SCNM1; NbExp=6; IntAct=EBI-10292696, EBI-748391;
CC Q96Q77; Q53GC0: SERTAD1; NbExp=3; IntAct=EBI-10292696, EBI-2826300;
CC Q96Q77; Q9UHV2: SERTAD1; NbExp=3; IntAct=EBI-10292696, EBI-748601;
CC Q96Q77; P03973: SLPI; NbExp=3; IntAct=EBI-10292696, EBI-355293;
CC Q96Q77; Q02447: SP3; NbExp=3; IntAct=EBI-10292696, EBI-348158;
CC Q96Q77; Q02446: SP4; NbExp=6; IntAct=EBI-10292696, EBI-10198587;
CC Q96Q77; Q16563: SYPL1; NbExp=3; IntAct=EBI-10292696, EBI-2800683;
CC Q96Q77; Q9Y6A5: TACC3; NbExp=9; IntAct=EBI-10292696, EBI-2554984;
CC Q96Q77; Q08117: TLE5; NbExp=3; IntAct=EBI-10292696, EBI-717810;
CC Q96Q77; Q08117-2: TLE5; NbExp=3; IntAct=EBI-10292696, EBI-11741437;
CC Q96Q77; O00463: TRAF5; NbExp=3; IntAct=EBI-10292696, EBI-523498;
CC Q96Q77; Q14142: TRIM14; NbExp=3; IntAct=EBI-10292696, EBI-2820256;
CC Q96Q77; Q9C040: TRIM2; NbExp=6; IntAct=EBI-10292696, EBI-749840;
CC Q96Q77; O43298: ZBTB43; NbExp=3; IntAct=EBI-10292696, EBI-740718;
CC Q96Q77; Q53FD0-2: ZC2HC1C; NbExp=3; IntAct=EBI-10292696, EBI-14104088;
CC Q96Q77; Q6P2D0: ZFP1; NbExp=3; IntAct=EBI-10292696, EBI-2555749;
CC Q96Q77; Q9NU63-3: ZFP57; NbExp=3; IntAct=EBI-10292696, EBI-12879708;
CC Q96Q77; Q15072: ZNF146; NbExp=3; IntAct=EBI-10292696, EBI-11914212;
CC Q96Q77; Q13360-2: ZNF177; NbExp=3; IntAct=EBI-10292696, EBI-12272076;
CC Q96Q77; Q9UJW8-4: ZNF180; NbExp=3; IntAct=EBI-10292696, EBI-12055755;
CC Q96Q77; P17023: ZNF19; NbExp=3; IntAct=EBI-10292696, EBI-12884200;
CC Q96Q77; Q8N554: ZNF276; NbExp=3; IntAct=EBI-10292696, EBI-750821;
CC Q96Q77; Q96PQ6: ZNF317; NbExp=5; IntAct=EBI-10292696, EBI-1210473;
CC Q96Q77; Q86UD4: ZNF329; NbExp=3; IntAct=EBI-10292696, EBI-7233259;
CC Q96Q77; Q8TD17: ZNF398; NbExp=6; IntAct=EBI-10292696, EBI-8643207;
CC Q96Q77; Q9BUY5: ZNF426; NbExp=3; IntAct=EBI-10292696, EBI-743265;
CC Q96Q77; Q9H707: ZNF552; NbExp=3; IntAct=EBI-10292696, EBI-2555731;
CC Q96Q77; Q96N58: ZNF578; NbExp=3; IntAct=EBI-10292696, EBI-11955189;
CC Q96Q77; Q96LX8: ZNF597; NbExp=3; IntAct=EBI-10292696, EBI-9091553;
CC Q96Q77; Q6ZNG0: ZNF620; NbExp=3; IntAct=EBI-10292696, EBI-4395669;
CC Q96Q77; Q8N3J9: ZNF664; NbExp=3; IntAct=EBI-10292696, EBI-2799450;
CC Q96Q77; P0C7X2: ZNF688; NbExp=3; IntAct=EBI-10292696, EBI-4395732;
CC Q96Q77; Q6NX45: ZNF774; NbExp=3; IntAct=EBI-10292696, EBI-10251462;
CC Q96Q77; Q9Y2P0: ZNF835; NbExp=3; IntAct=EBI-10292696, EBI-5667516;
CC Q96Q77; Q8NAM6: ZSCAN4; NbExp=3; IntAct=EBI-10292696, EBI-7252920;
CC Q96Q77; Q9BUG6: ZSCAN5A; NbExp=3; IntAct=EBI-10292696, EBI-8836980;
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q96Q77-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q96Q77-2; Sequence=VSP_055509;
CC -!- MISCELLANEOUS: The binding of either calcium or magnesium significantly
CC increases the structural stability of the protein in comparison to apo-
CC CIB (calcium- and magnesium-free form).
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DR EMBL; AB050868; BAB71789.1; -; mRNA.
DR EMBL; AC020911; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471106; EAW84535.1; -; Genomic_DNA.
DR EMBL; BC069428; AAH69428.1; -; mRNA.
DR EMBL; BC112200; AAI12201.1; -; mRNA.
DR EMBL; BC113591; AAI13592.1; -; mRNA.
DR CCDS; CCDS12340.1; -. [Q96Q77-1]
DR CCDS; CCDS74305.1; -. [Q96Q77-2]
DR RefSeq; NP_001287851.1; NM_001300922.1. [Q96Q77-2]
DR RefSeq; NP_473454.1; NM_054113.3. [Q96Q77-1]
DR PDB; 6WU5; X-ray; 1.88 A; A/B=1-187.
DR PDB; 6WU7; X-ray; 1.84 A; A/B=1-187.
DR PDB; 6WUD; X-ray; 1.84 A; A=1-187.
DR PDBsum; 6WU5; -.
DR PDBsum; 6WU7; -.
DR PDBsum; 6WUD; -.
DR AlphaFoldDB; Q96Q77; -.
DR SMR; Q96Q77; -.
DR BioGRID; 125586; 70.
DR IntAct; Q96Q77; 55.
DR STRING; 9606.ENSP00000269878; -.
DR iPTMnet; Q96Q77; -.
DR PhosphoSitePlus; Q96Q77; -.
DR BioMuta; CIB3; -.
DR MassIVE; Q96Q77; -.
DR PaxDb; Q96Q77; -.
DR PRIDE; Q96Q77; -.
DR Antibodypedia; 43661; 137 antibodies from 23 providers.
DR DNASU; 117286; -.
DR Ensembl; ENST00000269878.8; ENSP00000269878.3; ENSG00000141977.9. [Q96Q77-1]
DR Ensembl; ENST00000379859.7; ENSP00000369188.3; ENSG00000141977.9. [Q96Q77-2]
DR GeneID; 117286; -.
DR KEGG; hsa:117286; -.
DR MANE-Select; ENST00000269878.8; ENSP00000269878.3; NM_054113.4; NP_473454.1.
DR UCSC; uc002nds.4; human. [Q96Q77-1]
DR CTD; 117286; -.
DR DisGeNET; 117286; -.
DR GeneCards; CIB3; -.
DR HGNC; HGNC:24580; CIB3.
DR HPA; ENSG00000141977; Tissue enhanced (bone).
DR MIM; 610645; gene.
DR neXtProt; NX_Q96Q77; -.
DR OpenTargets; ENSG00000141977; -.
DR PharmGKB; PA134867741; -.
DR VEuPathDB; HostDB:ENSG00000141977; -.
DR eggNOG; KOG0038; Eukaryota.
DR GeneTree; ENSGT00940000160466; -.
DR HOGENOM; CLU_061288_6_0_1; -.
DR InParanoid; Q96Q77; -.
DR OMA; MEFEHVI; -.
DR OrthoDB; 1271942at2759; -.
DR PhylomeDB; Q96Q77; -.
DR TreeFam; TF313865; -.
DR PathwayCommons; Q96Q77; -.
DR SignaLink; Q96Q77; -.
DR BioGRID-ORCS; 117286; 12 hits in 1070 CRISPR screens.
DR ChiTaRS; CIB3; human.
DR GenomeRNAi; 117286; -.
DR Pharos; Q96Q77; Tbio.
DR PRO; PR:Q96Q77; -.
DR Proteomes; UP000005640; Chromosome 19.
DR RNAct; Q96Q77; protein.
DR Bgee; ENSG00000141977; Expressed in granulocyte and 75 other tissues.
DR ExpressionAtlas; Q96Q77; baseline and differential.
DR Genevisible; Q96Q77; HS.
DR GO; GO:0005509; F:calcium ion binding; IDA:UniProtKB.
DR GO; GO:0000287; F:magnesium ion binding; IDA:UniProtKB.
DR GO; GO:0055074; P:calcium ion homeostasis; IBA:GO_Central.
DR CDD; cd00051; EFh; 1.
DR InterPro; IPR011992; EF-hand-dom_pair.
DR InterPro; IPR018247; EF_Hand_1_Ca_BS.
DR InterPro; IPR002048; EF_hand_dom.
DR Pfam; PF13499; EF-hand_7; 1.
DR SUPFAM; SSF47473; SSF47473; 1.
DR PROSITE; PS00018; EF_HAND_1; 2.
DR PROSITE; PS50222; EF_HAND_2; 3.
PE 1: Evidence at protein level;
KW 3D-structure; Alternative splicing; Calcium; Magnesium; Metal-binding;
KW Reference proteome; Repeat.
FT CHAIN 1..187
FT /note="Calcium and integrin-binding family member 3"
FT /id="PRO_0000073536"
FT DOMAIN 66..101
FT /note="EF-hand 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 103..138
FT /note="EF-hand 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 144..179
FT /note="EF-hand 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 116
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 118
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 120
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 122
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 127
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 157
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 159
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 161
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 163
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 168
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT VAR_SEQ 18..66
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_055509"
FT VARIANT 139
FT /note="G -> E (in dbSNP:rs6512087)"
FT /evidence="ECO:0000269|PubMed:15489334"
FT /id="VAR_060268"
FT HELIX 10..19
FT /evidence="ECO:0007829|PDB:6WU7"
FT HELIX 24..37
FT /evidence="ECO:0007829|PDB:6WU7"
FT TURN 39..41
FT /evidence="ECO:0007829|PDB:6WU7"
FT HELIX 46..48
FT /evidence="ECO:0007829|PDB:6WUD"
FT HELIX 56..60
FT /evidence="ECO:0007829|PDB:6WU7"
FT TURN 63..67
FT /evidence="ECO:0007829|PDB:6WU7"
FT HELIX 71..78
FT /evidence="ECO:0007829|PDB:6WU7"
FT STRAND 82..84
FT /evidence="ECO:0007829|PDB:6WU7"
FT HELIX 88..98
FT /evidence="ECO:0007829|PDB:6WU7"
FT HELIX 104..115
FT /evidence="ECO:0007829|PDB:6WU7"
FT STRAND 120..123
FT /evidence="ECO:0007829|PDB:6WUD"
FT HELIX 125..135
FT /evidence="ECO:0007829|PDB:6WU7"
FT TURN 136..138
FT /evidence="ECO:0007829|PDB:6WU7"
FT HELIX 142..156
FT /evidence="ECO:0007829|PDB:6WU7"
FT STRAND 160..164
FT /evidence="ECO:0007829|PDB:6WU7"
FT HELIX 166..174
FT /evidence="ECO:0007829|PDB:6WU7"
FT HELIX 181..183
FT /evidence="ECO:0007829|PDB:6WU7"
SQ SEQUENCE 187 AA; 21802 MW; FB32CCB46DF5ADCF CRC64;
MGNKQTVFTH EQLEAYQDCT FFTRKEIMRL FYRYQDLAPQ LVPLDYTTCP DVKVPYELIG
SMPELKDNPF RQRIAQVFSE DGDGHMTLDN FLDMFSVMSE MAPRDLKAYY AFKIYDFNND
DYICAWDLEQ TVTKLTRGGL SAEEVSLVCE KVLDEADGDH DGRLSLEDFQ NMILRAPDFL
STFHIRI
