CIB3_MOUSE
ID CIB3_MOUSE Reviewed; 160 AA.
AC Q0P523;
DT 19-MAR-2014, integrated into UniProtKB/Swiss-Prot.
DT 19-SEP-2006, sequence version 1.
DT 03-AUG-2022, entry version 110.
DE RecName: Full=Calcium and integrin-binding family member 3;
GN Name=Cib3;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP INTERACTION WITH ITGA2B, AND TISSUE SPECIFICITY.
RX PubMed=18989529; DOI=10.1160/th08-06-0351;
RA Denofrio J.C., Yuan W., Temple B.R., Gentry H.R., Parise L.V.;
RT "Characterization of calcium- and integrin-binding protein 1 (CIB1)
RT knockout platelets: potential compensation by CIB family members.";
RL Thromb. Haemost. 100:847-856(2008).
CC -!- SUBUNIT: Interacts with ITGA2B (via C-terminus cytoplasmic tail
CC region); the interaction is stabilized/increased in a calcium and
CC magnesium-dependent manner. {ECO:0000269|PubMed:18989529}.
CC -!- TISSUE SPECIFICITY: Expressed in megakaryocytes and endothelial cells.
CC {ECO:0000269|PubMed:18989529}.
CC -!- MISCELLANEOUS: The binding of either calcium or magnesium significantly
CC increases the structural stability of the protein in comparison to apo-
CC CIB (calcium- and magnesium-free form). {ECO:0000250}.
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DR EMBL; AC158898; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC120812; AAI20813.1; -; mRNA.
DR EMBL; BC120814; AAI20815.1; -; mRNA.
DR CCDS; CCDS52594.1; -.
DR RefSeq; NP_001074281.1; NM_001080812.1.
DR AlphaFoldDB; Q0P523; -.
DR SMR; Q0P523; -.
DR STRING; 10090.ENSMUSP00000096231; -.
DR PhosphoSitePlus; Q0P523; -.
DR PaxDb; Q0P523; -.
DR PRIDE; Q0P523; -.
DR Antibodypedia; 43661; 137 antibodies from 23 providers.
DR Ensembl; ENSMUST00000098630; ENSMUSP00000096231; ENSMUSG00000074240.
DR GeneID; 234421; -.
DR KEGG; mmu:234421; -.
DR UCSC; uc009mfm.1; mouse.
DR CTD; 117286; -.
DR MGI; MGI:2685953; Cib3.
DR VEuPathDB; HostDB:ENSMUSG00000074240; -.
DR eggNOG; KOG0038; Eukaryota.
DR GeneTree; ENSGT00940000160466; -.
DR HOGENOM; CLU_061288_6_0_1; -.
DR InParanoid; Q0P523; -.
DR OrthoDB; 1271942at2759; -.
DR PhylomeDB; Q0P523; -.
DR TreeFam; TF313865; -.
DR BioGRID-ORCS; 234421; 2 hits in 73 CRISPR screens.
DR PRO; PR:Q0P523; -.
DR Proteomes; UP000000589; Chromosome 8.
DR RNAct; Q0P523; protein.
DR Bgee; ENSMUSG00000074240; Expressed in otolith organ and 22 other tissues.
DR ExpressionAtlas; Q0P523; baseline and differential.
DR GO; GO:0005509; F:calcium ion binding; ISS:UniProtKB.
DR GO; GO:0000287; F:magnesium ion binding; ISS:UniProtKB.
DR GO; GO:0055074; P:calcium ion homeostasis; IBA:GO_Central.
DR CDD; cd00051; EFh; 1.
DR InterPro; IPR011992; EF-hand-dom_pair.
DR InterPro; IPR018247; EF_Hand_1_Ca_BS.
DR InterPro; IPR002048; EF_hand_dom.
DR Pfam; PF13499; EF-hand_7; 1.
DR SUPFAM; SSF47473; SSF47473; 1.
DR PROSITE; PS00018; EF_HAND_1; 2.
DR PROSITE; PS50222; EF_HAND_2; 3.
PE 1: Evidence at protein level;
KW Calcium; Magnesium; Metal-binding; Reference proteome; Repeat.
FT CHAIN 1..160
FT /note="Calcium and integrin-binding family member 3"
FT /id="PRO_0000425745"
FT DOMAIN 39..74
FT /note="EF-hand 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 76..111
FT /note="EF-hand 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 117..152
FT /note="EF-hand 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 89
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 91
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 93
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 95
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 100
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 130
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 132
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 134
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 136
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 141
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
SQ SEQUENCE 160 AA; 18668 MW; 7010EEC986D97A7F CRC64;
MRLFYRYQDL APQLVPLDYT TSPKVKVPYE LIGSMPELKD NPFRQRIAQV FSQDGDGHMT
LENFLDMFSV MSEMAPRDLK AYYAFKIYDF NNDNYICAWD LEQTVTRLTR GELSAEEVTL
VCEKVLDEAD GDQDGRLSLE DFQNMILRAP DFLSTFHIRI
