CIB4_HUMAN
ID CIB4_HUMAN Reviewed; 185 AA.
AC A0PJX0; B2RU18;
DT 29-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 09-JAN-2007, sequence version 1.
DT 03-AUG-2022, entry version 110.
DE RecName: Full=Calcium and integrin-binding family member 4;
GN Name=CIB4;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND VARIANT ARG-181.
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND VARIANT ARG-181.
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP INTERACTION WITH ITGA2B, MAGNESIUM-BINDING, AND CALCIUM-BINDING.
RX PubMed=22779914; DOI=10.1139/o2012-021;
RA Huang H., Bogstie J.N., Vogel H.J.;
RT "Biophysical and structural studies of the human calcium- and integrin-
RT binding protein family: understanding their functional similarities and
RT differences.";
RL Biochem. Cell Biol. 90:646-656(2012).
CC -!- SUBUNIT: Interacts with ITGA2B (via C-terminus cytoplasmic tail
CC region); the interaction is stabilized/increased in a calcium- and
CC magnesium-dependent manner. {ECO:0000269|PubMed:22779914}.
CC -!- INTERACTION:
CC A0PJX0; Q7Z3C6-3: ATG9A; NbExp=5; IntAct=EBI-12868028, EBI-12006308;
CC A0PJX0; Q02930-3: CREB5; NbExp=3; IntAct=EBI-12868028, EBI-10192698;
CC A0PJX0; Q86VE0: MYPOP; NbExp=3; IntAct=EBI-12868028, EBI-2858213;
CC A0PJX0; Q9BSH4: TACO1; NbExp=3; IntAct=EBI-12868028, EBI-747797;
CC A0PJX0; Q99757: TXN2; NbExp=3; IntAct=EBI-12868028, EBI-2932492;
CC A0PJX0; Q6EMK4: VASN; NbExp=3; IntAct=EBI-12868028, EBI-10249550;
CC A0PJX0; Q96C00: ZBTB9; NbExp=3; IntAct=EBI-12868028, EBI-395708;
CC -!- MISCELLANEOUS: The binding of either calcium or magnesium may
CC significantly increases the structural stability of the protein in
CC comparison to apo-CIB (calcium- and magnesium-free form).
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DR EMBL; CH471053; EAX00680.1; -; Genomic_DNA.
DR EMBL; BC127684; AAI27685.1; -; mRNA.
DR EMBL; BC127685; AAI27686.1; -; mRNA.
DR EMBL; BC140923; AAI40924.1; -; mRNA.
DR CCDS; CCDS33160.1; -.
DR RefSeq; NP_001025052.1; NM_001029881.2.
DR AlphaFoldDB; A0PJX0; -.
DR SMR; A0PJX0; -.
DR BioGRID; 126223; 13.
DR IntAct; A0PJX0; 8.
DR STRING; 9606.ENSP00000288861; -.
DR iPTMnet; A0PJX0; -.
DR PhosphoSitePlus; A0PJX0; -.
DR BioMuta; CIB4; -.
DR MassIVE; A0PJX0; -.
DR PaxDb; A0PJX0; -.
DR PeptideAtlas; A0PJX0; -.
DR PRIDE; A0PJX0; -.
DR ProteomicsDB; 66; -.
DR Antibodypedia; 27982; 119 antibodies from 24 providers.
DR DNASU; 130106; -.
DR Ensembl; ENST00000288861.5; ENSP00000288861.4; ENSG00000157884.11.
DR GeneID; 130106; -.
DR KEGG; hsa:130106; -.
DR MANE-Select; ENST00000288861.5; ENSP00000288861.4; NM_001029881.3; NP_001025052.1.
DR UCSC; uc002rhm.4; human.
DR CTD; 130106; -.
DR DisGeNET; 130106; -.
DR GeneCards; CIB4; -.
DR HGNC; HGNC:33703; CIB4.
DR HPA; ENSG00000157884; Tissue enriched (testis).
DR MIM; 610646; gene.
DR neXtProt; NX_A0PJX0; -.
DR OpenTargets; ENSG00000157884; -.
DR PharmGKB; PA162382288; -.
DR VEuPathDB; HostDB:ENSG00000157884; -.
DR eggNOG; KOG0034; Eukaryota.
DR GeneTree; ENSGT00940000161840; -.
DR HOGENOM; CLU_061288_6_2_1; -.
DR InParanoid; A0PJX0; -.
DR OMA; CKVFSHN; -.
DR OrthoDB; 1271942at2759; -.
DR PhylomeDB; A0PJX0; -.
DR TreeFam; TF313865; -.
DR PathwayCommons; A0PJX0; -.
DR SignaLink; A0PJX0; -.
DR BioGRID-ORCS; 130106; 7 hits in 1057 CRISPR screens.
DR ChiTaRS; CIB4; human.
DR GenomeRNAi; 130106; -.
DR Pharos; A0PJX0; Tbio.
DR PRO; PR:A0PJX0; -.
DR Proteomes; UP000005640; Chromosome 2.
DR RNAct; A0PJX0; protein.
DR Bgee; ENSG00000157884; Expressed in left testis and 96 other tissues.
DR Genevisible; A0PJX0; HS.
DR GO; GO:0005509; F:calcium ion binding; IDA:UniProtKB.
DR GO; GO:0000287; F:magnesium ion binding; IDA:UniProtKB.
DR CDD; cd00051; EFh; 1.
DR InterPro; IPR011992; EF-hand-dom_pair.
DR InterPro; IPR018247; EF_Hand_1_Ca_BS.
DR InterPro; IPR002048; EF_hand_dom.
DR Pfam; PF13499; EF-hand_7; 1.
DR SMART; SM00054; EFh; 2.
DR SUPFAM; SSF47473; SSF47473; 1.
DR PROSITE; PS00018; EF_HAND_1; 2.
DR PROSITE; PS50222; EF_HAND_2; 2.
PE 1: Evidence at protein level;
KW Calcium; Magnesium; Metal-binding; Reference proteome; Repeat.
FT CHAIN 1..185
FT /note="Calcium and integrin-binding family member 4"
FT /id="PRO_0000289290"
FT DOMAIN 62..95
FT /note="EF-hand 1"
FT /evidence="ECO:0000305"
FT DOMAIN 97..132
FT /note="EF-hand 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 139..174
FT /note="EF-hand 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 110
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 112
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 114
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 121
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 152
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 154
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 156
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 158
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 163
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT VARIANT 181
FT /note="H -> R (in dbSNP:rs935172)"
FT /evidence="ECO:0000269|PubMed:15489334, ECO:0000269|Ref.1"
FT /id="VAR_048637"
SQ SEQUENCE 185 AA; 21745 MW; A0DB9D75BF8F9724 CRC64;
MGQCLRYQMH WEDLEEYQAL TFLTRNEILC IHDTFLKLCP PGKYYKEATL TMDQVSSLPA
LRVNPFRDRI CRVFSHKGMF SFEDVLGMAS VFSEQACPSL KIEYAFRIYD FNENGFIDEE
DLQRIILRLL NSDDMSEDLL MDLTNHVLSE SDLDNDNMLS FSEFEHAMAK SPDFMNSFRI
HFWGC
