CIB4_MOUSE
ID CIB4_MOUSE Reviewed; 185 AA.
AC Q9D9N5;
DT 29-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 133.
DE RecName: Full=Calcium and integrin-binding family member 4;
GN Name=Cib4;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP TISSUE SPECIFICITY.
RX PubMed=18989529; DOI=10.1160/th08-06-0351;
RA Denofrio J.C., Yuan W., Temple B.R., Gentry H.R., Parise L.V.;
RT "Characterization of calcium- and integrin-binding protein 1 (CIB1)
RT knockout platelets: potential compensation by CIB family members.";
RL Thromb. Haemost. 100:847-856(2008).
CC -!- SUBUNIT: Interacts with ITGA2B (via C-terminus cytoplasmic tail
CC region); the interaction is stabilized/increased in a calcium- and
CC magnesium-dependent manner. {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Expressed weakly in megakaryocytes and endothelial
CC cells. {ECO:0000269|PubMed:18989529}.
CC -!- MISCELLANEOUS: The binding of either calcium or magnesium may
CC significantly increases the structural stability of the protein in
CC comparison to apo-CIB (calcium- and magnesium-free form).
CC {ECO:0000250}.
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DR EMBL; BC118938; AAI18939.1; -; mRNA.
DR EMBL; AK006670; BAB24697.1; -; mRNA.
DR CCDS; CCDS51453.1; -.
DR RefSeq; NP_082759.1; NM_028483.1.
DR AlphaFoldDB; Q9D9N5; -.
DR SMR; Q9D9N5; -.
DR STRING; 10090.ENSMUSP00000066222; -.
DR iPTMnet; Q9D9N5; -.
DR PhosphoSitePlus; Q9D9N5; -.
DR PaxDb; Q9D9N5; -.
DR PRIDE; Q9D9N5; -.
DR ProteomicsDB; 283555; -.
DR Antibodypedia; 27982; 119 antibodies from 24 providers.
DR Ensembl; ENSMUST00000065486; ENSMUSP00000066222; ENSMUSG00000053194.
DR GeneID; 73259; -.
DR KEGG; mmu:73259; -.
DR UCSC; uc008wvo.1; mouse.
DR CTD; 130106; -.
DR MGI; MGI:1920509; Cib4.
DR VEuPathDB; HostDB:ENSMUSG00000053194; -.
DR eggNOG; KOG0034; Eukaryota.
DR GeneTree; ENSGT00940000161840; -.
DR HOGENOM; CLU_061288_6_2_1; -.
DR InParanoid; Q9D9N5; -.
DR OMA; CKVFSHN; -.
DR OrthoDB; 1271942at2759; -.
DR PhylomeDB; Q9D9N5; -.
DR TreeFam; TF313865; -.
DR BioGRID-ORCS; 73259; 4 hits in 74 CRISPR screens.
DR ChiTaRS; Cib4; mouse.
DR PRO; PR:Q9D9N5; -.
DR Proteomes; UP000000589; Chromosome 5.
DR RNAct; Q9D9N5; protein.
DR Bgee; ENSMUSG00000053194; Expressed in spermatid and 6 other tissues.
DR Genevisible; Q9D9N5; MM.
DR GO; GO:0005509; F:calcium ion binding; ISS:UniProtKB.
DR GO; GO:0000287; F:magnesium ion binding; ISS:UniProtKB.
DR CDD; cd00051; EFh; 1.
DR InterPro; IPR011992; EF-hand-dom_pair.
DR InterPro; IPR018247; EF_Hand_1_Ca_BS.
DR InterPro; IPR002048; EF_hand_dom.
DR Pfam; PF13499; EF-hand_7; 1.
DR SMART; SM00054; EFh; 2.
DR SUPFAM; SSF47473; SSF47473; 1.
DR PROSITE; PS00018; EF_HAND_1; 1.
DR PROSITE; PS50222; EF_HAND_2; 2.
PE 2: Evidence at transcript level;
KW Calcium; Magnesium; Metal-binding; Reference proteome; Repeat.
FT CHAIN 1..185
FT /note="Calcium and integrin-binding family member 4"
FT /id="PRO_0000289291"
FT DOMAIN 62..95
FT /note="EF-hand 1"
FT /evidence="ECO:0000305"
FT DOMAIN 97..132
FT /note="EF-hand 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 138..174
FT /note="EF-hand 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 110
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 112
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 114
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 121
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
SQ SEQUENCE 185 AA; 21637 MW; 70C6B3EBF73844CB CRC64;
MGQCLRYQMQ WEDLEEYQAL TFLTRNEILC IHDTFLKLCP SGKHYKEATL TMDQVSSLPA
LRVNPFRDRI CRVFSHDNVF SFEDVLGMAS VFSEQACPSL KIEYAFRIYD FNENGFIDEE
DLEEIVLRLL KSDDASEDLL MDVMHHVLSE SDLDNDSMLS FSEFEHAMAK SPDFMNSFRI
HFWGC
