CIB4_SHEEP
ID CIB4_SHEEP Reviewed; 185 AA.
AC C7A278;
DT 19-MAR-2014, integrated into UniProtKB/Swiss-Prot.
DT 22-SEP-2009, sequence version 1.
DT 03-AUG-2022, entry version 43.
DE RecName: Full=Calcium and integrin-binding family member 4;
GN Name=CIB4;
OS Ovis aries (Sheep).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Caprinae; Ovis.
OX NCBI_TaxID=9940;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), AND TISSUE SPECIFICITY.
RC TISSUE=Testis;
RX PubMed=18989754; DOI=10.1007/s11033-008-9383-4;
RA Yu Y., Song X., Du L., Wang C.;
RT "Molecular characterization of the sheep CIB1 gene.";
RL Mol. Biol. Rep. 36:1799-1809(2009).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), ALTERNATIVE SPLICING (ISOFORM 2),
RP AND TISSUE SPECIFICITY.
RX PubMed=20236775; DOI=10.1016/j.anireprosci.2010.01.004;
RA Yu Y., Zhang Y., Song X., Jin M., Guan Q., Zhang Q., Li S., Wei C., Lu G.,
RA Zhang J., Ren H., Sheng X., Wang C., Du L.;
RT "Alternative splicing and tissue expression of CIB4 gene in sheep testis.";
RL Anim. Reprod. Sci. 120:1-9(2010).
CC -!- SUBUNIT: Interacts with ITGA2B (via C-terminus cytoplasmic tail
CC region); the interaction is stabilized/increased in a calcium- and
CC magnesium-dependent manner. {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1; Synonyms=L-CIB4;
CC IsoId=C7A278-1; Sequence=Displayed;
CC Name=2; Synonyms=S-CIB4;
CC IsoId=C7A278-2; Sequence=VSP_053853;
CC -!- TISSUE SPECIFICITY: Isoform 1 and isoform 2 are expressed in testis.
CC {ECO:0000269|PubMed:18989754, ECO:0000269|PubMed:20236775}.
CC -!- MISCELLANEOUS: The binding of either calcium or magnesium may
CC significantly increases the structural stability of the protein in
CC comparison to apo-CIB (calcium- and magnesium-free form).
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DR EMBL; FJ039532; ACM89974.1; -; mRNA.
DR RefSeq; NP_001158185.1; NM_001164713.1. [C7A278-1]
DR AlphaFoldDB; C7A278; -.
DR SMR; C7A278; -.
DR STRING; 9940.ENSOARP00000020365; -.
DR PRIDE; C7A278; -.
DR GeneID; 100303602; -.
DR KEGG; oas:100303602; -.
DR CTD; 130106; -.
DR eggNOG; KOG0034; Eukaryota.
DR Proteomes; UP000002356; Unplaced.
DR GO; GO:0005509; F:calcium ion binding; ISS:UniProtKB.
DR GO; GO:0000287; F:magnesium ion binding; ISS:UniProtKB.
DR CDD; cd00051; EFh; 1.
DR InterPro; IPR011992; EF-hand-dom_pair.
DR InterPro; IPR018247; EF_Hand_1_Ca_BS.
DR InterPro; IPR002048; EF_hand_dom.
DR Pfam; PF13499; EF-hand_7; 1.
DR SMART; SM00054; EFh; 2.
DR SUPFAM; SSF47473; SSF47473; 1.
DR PROSITE; PS00018; EF_HAND_1; 2.
DR PROSITE; PS50222; EF_HAND_2; 2.
PE 2: Evidence at transcript level;
KW Alternative splicing; Calcium; Magnesium; Metal-binding;
KW Reference proteome; Repeat.
FT CHAIN 1..185
FT /note="Calcium and integrin-binding family member 4"
FT /id="PRO_0000425746"
FT DOMAIN 97..132
FT /note="EF-hand 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 147..174
FT /note="EF-hand 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 110
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 112
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 114
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 121
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 152
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 154
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 156
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 158
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 163
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT VAR_SEQ 63..185
FT /note="VNPFRDRICRVFSHNNVFSFEDVLGMASVFSEQACPSLKIEYAFRIYDFNEN
FT GFIDEEDLQRIILRLLNSDDMSEDLLTDLTSHVLNESDLDNDNMLSFSEFEHAMAKSPD
FT FMNSFRIHFWGC -> ILMRTASLTRRTCRGSSSGCSTVTTCQKTC (in isoform
FT 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_053853"
SQ SEQUENCE 185 AA; 21679 MW; 4BB04D41B96B1AFA CRC64;
MGQCLRYQMH WEDLEEYQAL TFLTRNEILC IHDSFLKLCP PGKYYKEATL TVDQVSSLPA
LRVNPFRDRI CRVFSHNNVF SFEDVLGMAS VFSEQACPSL KIEYAFRIYD FNENGFIDEE
DLQRIILRLL NSDDMSEDLL TDLTSHVLNE SDLDNDNMLS FSEFEHAMAK SPDFMNSFRI
HFWGC
