CICB_EMENI
ID CICB_EMENI Reviewed; 1039 AA.
AC A0A1U8QW91; C8V0E0; Q5AZ36;
DT 07-OCT-2020, integrated into UniProtKB/Swiss-Prot.
DT 10-MAY-2017, sequence version 1.
DT 03-AUG-2022, entry version 20.
DE RecName: Full=Adenylate-forming reductase cicB {ECO:0000250|UniProtKB:Q0CRQ4};
DE EC=1.2.1.- {ECO:0000250|UniProtKB:Q0CRQ4};
DE AltName: Full=Cichorine biosynthesis cluster protein B {ECO:0000303|PubMed:24244835};
DE AltName: Full=Nonribosomal peptide synthase-like protein cicB {ECO:0000250|UniProtKB:Q0CRQ4};
DE Short=NRPS-like protein cicB {ECO:0000250|UniProtKB:Q0CRQ4};
GN Name=cicB {ECO:0000303|PubMed:24244835}; ORFNames=AN6444, ANIA_06444;
OS Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 /
OS M139) (Aspergillus nidulans).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC Aspergillus subgen. Nidulantes.
OX NCBI_TaxID=227321;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139;
RX PubMed=16372000; DOI=10.1038/nature04341;
RA Galagan J.E., Calvo S.E., Cuomo C., Ma L.-J., Wortman J.R., Batzoglou S.,
RA Lee S.-I., Bastuerkmen M., Spevak C.C., Clutterbuck J., Kapitonov V.,
RA Jurka J., Scazzocchio C., Farman M.L., Butler J., Purcell S., Harris S.,
RA Braus G.H., Draht O., Busch S., D'Enfert C., Bouchier C., Goldman G.H.,
RA Bell-Pedersen D., Griffiths-Jones S., Doonan J.H., Yu J., Vienken K.,
RA Pain A., Freitag M., Selker E.U., Archer D.B., Penalva M.A., Oakley B.R.,
RA Momany M., Tanaka T., Kumagai T., Asai K., Machida M., Nierman W.C.,
RA Denning D.W., Caddick M.X., Hynes M., Paoletti M., Fischer R., Miller B.L.,
RA Dyer P.S., Sachs M.S., Osmani S.A., Birren B.W.;
RT "Sequencing of Aspergillus nidulans and comparative analysis with A.
RT fumigatus and A. oryzae.";
RL Nature 438:1105-1115(2005).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139;
RX PubMed=19146970; DOI=10.1016/j.fgb.2008.12.003;
RA Wortman J.R., Gilsenan J.M., Joardar V., Deegan J., Clutterbuck J.,
RA Andersen M.R., Archer D., Bencina M., Braus G., Coutinho P., von Dohren H.,
RA Doonan J., Driessen A.J., Durek P., Espeso E., Fekete E., Flipphi M.,
RA Estrada C.G., Geysens S., Goldman G., de Groot P.W., Hansen K.,
RA Harris S.D., Heinekamp T., Helmstaedt K., Henrissat B., Hofmann G.,
RA Homan T., Horio T., Horiuchi H., James S., Jones M., Karaffa L.,
RA Karanyi Z., Kato M., Keller N., Kelly D.E., Kiel J.A., Kim J.M.,
RA van der Klei I.J., Klis F.M., Kovalchuk A., Krasevec N., Kubicek C.P.,
RA Liu B., Maccabe A., Meyer V., Mirabito P., Miskei M., Mos M., Mullins J.,
RA Nelson D.R., Nielsen J., Oakley B.R., Osmani S.A., Pakula T., Paszewski A.,
RA Paulsen I., Pilsyk S., Pocsi I., Punt P.J., Ram A.F., Ren Q., Robellet X.,
RA Robson G., Seiboth B., van Solingen P., Specht T., Sun J.,
RA Taheri-Talesh N., Takeshita N., Ussery D., vanKuyk P.A., Visser H.,
RA van de Vondervoort P.J., de Vries R.P., Walton J., Xiang X., Xiong Y.,
RA Zeng A.P., Brandt B.W., Cornell M.J., van den Hondel C.A., Visser J.,
RA Oliver S.G., Turner G.;
RT "The 2008 update of the Aspergillus nidulans genome annotation: a community
RT effort.";
RL Fungal Genet. Biol. 46:S2-13(2009).
RN [3]
RP FUNCTION.
RX PubMed=22510154; DOI=10.1021/ja3016395;
RA Ahuja M., Chiang Y.M., Chang S.L., Praseuth M.B., Entwistle R.,
RA Sanchez J.F., Lo H.C., Yeh H.H., Oakley B.R., Wang C.C.;
RT "Illuminating the diversity of aromatic polyketide synthases in Aspergillus
RT nidulans.";
RL J. Am. Chem. Soc. 134:8212-8221(2012).
RN [4]
RP FUNCTION, DISRUPTION PHENOTYPE, AND PATHWAY.
RX PubMed=24244835; DOI=10.1039/c2md20055d;
RA Sanchez J.F., Entwistle R., Corcoran D., Oakley B.R., Wang C.C.;
RT "Identification and molecular genetic analysis of the cichorine gene
RT cluster in Aspergillus nidulans.";
RL Med. Chem. Commun. 3:0-0(2012).
RN [5]
RP BIOTECHNOLOGY.
RX PubMed=30708999; DOI=10.3390/molecules24030515;
RA Liao L., Zhang X., Lou Y., Zhou C., Yuan Q., Gao J.;
RT "Discovery of three new phytotoxins from the fungus Aspergillus nidulans by
RT pathway inactivation.";
RL Molecules 24:0-0(2019).
CC -!- FUNCTION: Nonribosomal peptide synthase-like protein; part of the gene
CC cluster that mediates the biosynthesis of cichorine, a phytotoxin
CC active against knapweed, corn, and soybeans (PubMed:24244835). The
CC first step in the pathway is performed by the non-reducing polyketide
CC synthase pkbA that condenses one acetyl-CoA starter unit with 3
CC malonyl-CoA units (PubMed:22510154). PkbA also catalyzes one
CC methylation step to produce 3-methylorsellinate (PubMed:22510154). The
CC nonribosomal peptide synthase-like protein cicB, the cytochrome P450
CC monooxygenase cicH and the O-methyltransferase cicE are involved in the
CC conversion of 3-methylorsellinate into nidulol (PubMed:24244835). CicB
CC converts 3-methylorsellinate to a yet unidentified intermediate, cicH
CC may play a ring-closing role for cichorine and cicE is plausibly
CC responsible for the methylation of one of the phenol groups (Probable).
CC The oxidoreductase cicC acts downstream with still unidentified enzymes
CC to further convert nidulol into cichorine (PubMed:24244835).
CC {ECO:0000269|PubMed:22510154, ECO:0000269|PubMed:24244835,
CC ECO:0000305|PubMed:24244835}.
CC -!- PATHWAY: Phytotoxin biosynthesis. {ECO:0000269|PubMed:24244835}.
CC -!- DOMAIN: Contains three distinct domains: an adenylation (A) domain that
CC activates the substrate amino acid which is subsequently covalently
CC linked as a thioester (aminoacyl-S-PCP) to the 4'-phosphopantetheine
CC prosthetic group of the second domain, the peptidyl carrier protein
CC (PCP) domain, as well as a reductase (R) release domain.
CC {ECO:0000250|UniProtKB:Q0CRQ4}.
CC -!- DISRUPTION PHENOTYPE: Abolishes the production of cichorine and leads
CC to the accumulation of 3-methylorsellinic acid.
CC {ECO:0000269|PubMed:24244835}.
CC -!- BIOTECHNOLOGY: Cichorine and its derivatives are promising in the
CC course of developing novel herbicides. {ECO:0000269|PubMed:30708999}.
CC -!- SIMILARITY: Belongs to the adenylate-forming reductase family.
CC {ECO:0000305}.
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DR EMBL; BN001301; CBF69459.1; -; Genomic_DNA.
DR EMBL; AACD01000108; EAA58466.1; -; Genomic_DNA.
DR RefSeq; XP_664048.1; XM_658956.1.
DR AlphaFoldDB; A0A1U8QW91; -.
DR SMR; A0A1U8QW91; -.
DR STRING; 162425.CADANIAP00006531; -.
DR EnsemblFungi; CBF69459; CBF69459; ANIA_06444.
DR EnsemblFungi; EAA58466; EAA58466; AN6444.2.
DR GeneID; 2871339; -.
DR KEGG; ani:AN6444.2; -.
DR VEuPathDB; FungiDB:AN6444; -.
DR eggNOG; KOG1178; Eukaryota.
DR HOGENOM; CLU_002220_2_1_1; -.
DR OMA; YDWKYFR; -.
DR OrthoDB; 229565at2759; -.
DR Proteomes; UP000000560; Chromosome I.
DR Proteomes; UP000005890; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR GO; GO:0062032; P:cichorine biosynthetic process; IMP:GO_Central.
DR Gene3D; 3.40.50.12780; -; 1.
DR InterPro; IPR020845; AMP-binding_CS.
DR InterPro; IPR000873; AMP-dep_Synth/Lig.
DR InterPro; IPR042099; ANL_N_sf.
DR InterPro; IPR013120; Far_NAD-bd.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR Pfam; PF00501; AMP-binding; 1.
DR Pfam; PF07993; NAD_binding_4; 1.
DR SUPFAM; SSF51735; SSF51735; 1.
DR PROSITE; PS00455; AMP_BINDING; 1.
PE 1: Evidence at protein level;
KW ATP-binding; NADP; Nucleotide-binding; Oxidoreductase; Phosphopantetheine;
KW Phosphoprotein; Reference proteome.
FT CHAIN 1..1039
FT /note="Adenylate-forming reductase cicB"
FT /id="PRO_0000450884"
FT DOMAIN 567..649
FT /note="Carrier"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
FT REGION 91..366
FT /note="Adenylation (A) domain"
FT /evidence="ECO:0000250|UniProtKB:Q0CRQ4, ECO:0000255"
FT REGION 685..910
FT /note="Reductase (R) domain"
FT /evidence="ECO:0000250|UniProtKB:Q0CRQ4, ECO:0000255"
FT BINDING 256
FT /ligand="AMP"
FT /ligand_id="ChEBI:CHEBI:456215"
FT /evidence="ECO:0000250|UniProtKB:Q6RKB1"
FT BINDING 350..351
FT /ligand="AMP"
FT /ligand_id="ChEBI:CHEBI:456215"
FT /evidence="ECO:0000250|UniProtKB:Q6RKB1"
FT BINDING 355
FT /ligand="AMP"
FT /ligand_id="ChEBI:CHEBI:456215"
FT /evidence="ECO:0000250|UniProtKB:Q6RKB1"
FT BINDING 429..432
FT /ligand="AMP"
FT /ligand_id="ChEBI:CHEBI:456215"
FT /evidence="ECO:0000250|UniProtKB:Q6RKB1"
FT BINDING 689..692
FT /ligand="NADP(+)"
FT /ligand_id="ChEBI:CHEBI:58349"
FT /evidence="ECO:0000250|UniProtKB:Q6RKB1"
FT BINDING 777..779
FT /ligand="NADP(+)"
FT /ligand_id="ChEBI:CHEBI:58349"
FT /evidence="ECO:0000250|UniProtKB:Q6RKB1"
FT BINDING 855
FT /ligand="NADP(+)"
FT /ligand_id="ChEBI:CHEBI:58349"
FT /evidence="ECO:0000250|UniProtKB:Q6RKB1"
FT BINDING 859
FT /ligand="NADP(+)"
FT /ligand_id="ChEBI:CHEBI:58349"
FT /evidence="ECO:0000250|UniProtKB:Q6RKB1"
FT MOD_RES 600
FT /note="O-(pantetheine 4'-phosphoryl)serine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
SQ SEQUENCE 1039 AA; 114875 MW; 1C3C0C9C7B13F571 CRC64;
MARNQQLRPF KRPAIRLYPA VNETELIRTL PEVVEFNAQA NPDHVFCLQA KKSPNKSILS
LVSVTNRQLK QAISQCVGWL KSNLKLQLPS LLHGSVEKGA PVALLMESDI GLFIYEIALI
GLGVPVLLLS ARLSAPAINS LLQRTSAAAI IVSGRLEATA REAAETLPAA VFSPLPFEAF
LSTNEGLPDN DSICHPYHYI DESDRNVLIL HSSGTTGLPK PIYVSHRHLL SFVNCHRLDL
ESEAQGVNVS TLPLYHGFGL VAPALAMGVG KTVCFPPGST VPSAGATLDL LRISNAASFM
TVPSILEDIA FLGDEGIQAL AGLDYVTFGG GILKPSVGEE LAVHGVKLLN HYGTTESGPL
APIFVPQDDY NWRFFRLRDD LRLQLDEIAP NGNERRFKLT TFPFGLEQPF EIQDQLVCNP
DYPGSDFNAV GRNDDTIVLA TGEKVQPQIL ESILCESSLV KSAIAFGEHQ FEIGVLVQLA
ADVPPKNYSK LREQLWPLVA RANESMDGHA RIQSSEAVLL LPSTVTIPRT DKGSIARKDV
YKMFEADIAG VYRKLESAGP VLCLDMETLE EDLKLLISRC TDWPPDWAVT DDLFERGMNS
LQAIRVQRAL VAAVTRSLRG VCQPERIGRD FVYIHPSVRA MADFFREPVN GLQLPNGFST
RGWAPDQLVQ RFALTSKDGR ATVLLTGATG SLGSHCLLSL IQSPNIKRVI CLVRPEDSFT
DPRLRLQKSL ESKKLHLSST QWSMVDVLGC HTASQYLGLT REQYTILQES VTYILHAAWP
MDFHWKLPSF QSQFQSLHNL LALARDIHNR RPSIKPRLTF ISSIATVGQY ARVHGVRMVP
ETSVDSVECL NPIGYAEAKL VCERMLEHAR LHYPHEMTVS YVRMGQIAGS STTGFWNINE
HIPALTLSWI PVDVAAQSIT ELLLSPAPAE LIYHIENPIR QSWHDMLQTI AAVLHLSASD
ALPWNEWQEQ VAAAGDTDNP AKKLQDFFAN DFIRMGCGEV VLGTDKARKA SATLRRVDAV
SEGCVRGYLQ YWKEIGWLK