CID8_ARATH
ID CID8_ARATH Reviewed; 314 AA.
AC Q9C8M0; F4HRL0; Q67ZZ8;
DT 14-MAY-2014, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 139.
DE RecName: Full=Polyadenylate-binding protein-interacting protein 8;
DE Short=PABP-interacting protein 8;
DE Short=Poly(A)-binding protein-interacting protein 8;
DE AltName: Full=PAM2-containing protein CID8;
DE AltName: Full=Protein CTC-INTERACTING DOMAIN 8;
GN Name=CID8; OrderedLocusNames=At1g53650; ORFNames=F22G10.7;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RA Cheuk R., Chen H., Kim C.J., Shinn P., Ecker J.R.;
RT "Arabidosis ORF clones.";
RL Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 14-314 (ISOFORM 2).
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP GENE FAMILY, PAM2 MOTIF, AND TISSUE SPECIFICITY.
RX PubMed=15650869; DOI=10.1007/s00438-004-1090-9;
RA Bravo J., Aguilar-Henonin L., Olmedo G., Guzman P.;
RT "Four distinct classes of proteins as interaction partners of the PABC
RT domain of Arabidopsis thaliana Poly(A)-binding proteins.";
RL Mol. Genet. Genomics 272:651-665(2005).
RN [6]
RP INTERACTION WITH MPC.
RX PubMed=18796636; DOI=10.1105/tpc.108.061929;
RA Tiwari S., Schulz R., Ikeda Y., Dytham L., Bravo J., Mathers L.,
RA Spielman M., Guzman P., Oakey R.J., Kinoshita T., Scott R.J.;
RT "MATERNALLY EXPRESSED PAB C-TERMINAL, a novel imprinted gene in
RT Arabidopsis, encodes the conserved C-terminal domain of polyadenylate
RT binding proteins.";
RL Plant Cell 20:2387-2398(2008).
CC -!- SUBUNIT: Interacts with MPC. {ECO:0000269|PubMed:18796636}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9C8M0-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9C8M0-2; Sequence=VSP_054321;
CC -!- TISSUE SPECIFICITY: Expressed in cauline leaves, stems, rosette leaves,
CC immature siliques and primary inflorescences but at a low level.
CC {ECO:0000269|PubMed:15650869}.
CC -!- DOMAIN: Contains a PAM2-like motif, which seems to be involved in the
CC binding to the PABC/CTC domain of PAB proteins.
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DR EMBL; AC024260; AAG51971.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE32976.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE32977.1; -; Genomic_DNA.
DR EMBL; BT014885; AAT42377.1; -; mRNA.
DR EMBL; AK175783; BAD43546.1; -; mRNA.
DR EMBL; AK175969; BAD43732.1; -; mRNA.
DR PIR; F96576; F96576.
DR RefSeq; NP_001031182.1; NM_001036105.1. [Q9C8M0-2]
DR RefSeq; NP_175769.1; NM_104243.4. [Q9C8M0-1]
DR AlphaFoldDB; Q9C8M0; -.
DR SMR; Q9C8M0; -.
DR BioGRID; 27027; 3.
DR IntAct; Q9C8M0; 2.
DR STRING; 3702.AT1G53650.1; -.
DR PaxDb; Q9C8M0; -.
DR PRIDE; Q9C8M0; -.
DR ProteomicsDB; 246942; -. [Q9C8M0-1]
DR EnsemblPlants; AT1G53650.1; AT1G53650.1; AT1G53650. [Q9C8M0-1]
DR EnsemblPlants; AT1G53650.2; AT1G53650.2; AT1G53650. [Q9C8M0-2]
DR GeneID; 841802; -.
DR Gramene; AT1G53650.1; AT1G53650.1; AT1G53650. [Q9C8M0-1]
DR Gramene; AT1G53650.2; AT1G53650.2; AT1G53650. [Q9C8M0-2]
DR KEGG; ath:AT1G53650; -.
DR Araport; AT1G53650; -.
DR TAIR; locus:2024842; AT1G53650.
DR eggNOG; KOG0118; Eukaryota.
DR InParanoid; Q9C8M0; -.
DR OMA; SCDDFAT; -.
DR OrthoDB; 1342709at2759; -.
DR PhylomeDB; Q9C8M0; -.
DR PRO; PR:Q9C8M0; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9C8M0; baseline and differential.
DR Genevisible; Q9C8M0; AT.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR CDD; cd12459; RRM1_CID8_like; 1.
DR CDD; cd12460; RRM2_CID8_like; 1.
DR Gene3D; 3.30.70.330; -; 2.
DR InterPro; IPR009818; Ataxin-2_C.
DR InterPro; IPR034823; CID8-like_RRM1.
DR InterPro; IPR034825; CID8-like_RRM2.
DR InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf.
DR InterPro; IPR035979; RBD_domain_sf.
DR InterPro; IPR000504; RRM_dom.
DR Pfam; PF07145; PAM2; 1.
DR Pfam; PF00076; RRM_1; 2.
DR SMART; SM00360; RRM; 2.
DR SUPFAM; SSF54928; SSF54928; 2.
DR PROSITE; PS50102; RRM; 2.
PE 1: Evidence at protein level;
KW Alternative splicing; Nucleus; Reference proteome; Repeat; RNA-binding.
FT CHAIN 1..314
FT /note="Polyadenylate-binding protein-interacting protein 8"
FT /id="PRO_0000428898"
FT DOMAIN 128..203
FT /note="RRM 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT DOMAIN 225..301
FT /note="RRM 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT REGION 1..47
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 59..69
FT /note="PAM2-like"
FT MOTIF 99..112
FT /note="Bipartite nuclear localization signal"
FT /evidence="ECO:0000255"
FT COMPBIAS 24..47
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 95..100
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|Ref.4"
FT /id="VSP_054321"
SQ SEQUENCE 314 AA; 34682 MW; 64B3CA9E19796A62 CRC64;
MAAITEMATD SNDVINDGGT GDGIEKSTDS KPEIESDDLK PKSKPEYDQM KKLVAMFKKL
NPEAKEFFPS YKRNTNQSDD FVIAIKPSGE DNKKVAINRR RRNNYNQGRR VRLPGRASKA
QREDSIRRTV YVSDIDQSVT EEGLAGLFSS CGQVVDCRIC GDPNSVLRFA FVEFSDDQGA
RSALSLGGTM IGYYPVRVLP SKTAILPVNP TFLPRSEDER EMCSRTIYCT NVDKNATEDD
VNTFFQSACG EVTRLRLLGD QVHSTRIAFV EFAMAESAVA ALNCSGIVLG SQPIRVSPSK
TPVRSRITRS PSPN
