CIF1_ARATH
ID CIF1_ARATH Reviewed; 83 AA.
AC Q84MD2;
DT 12-APR-2017, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 03-AUG-2022, entry version 109.
DE RecName: Full=Protein CASPARIAN STRIP INTEGRITY FACTOR 1 {ECO:0000303|PubMed:28104889};
DE Flags: Precursor;
GN Name=CIF1 {ECO:0000303|PubMed:28104889};
GN OrderedLocusNames=At2g16385 {ECO:0000312|Araport:AT2G16385};
GN ORFNames=F16F14 {ECO:0000305};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE,
RP SULFATION AT TYR-64, HYDROXYLATION AT PRO-69 AND PRO-71, INTERACTION WITH
RP GSO1 AND GSO2, AND INDUCTION BY IRON.
RX PubMed=28104889; DOI=10.1126/science.aai9057;
RA Nakayama T., Shinohara H., Tanaka M., Baba K., Ogawa-Ohnishi M.,
RA Matsubayashi Y.;
RT "A peptide hormone required for Casparian strip diffusion barrier formation
RT in Arabidopsis roots.";
RL Science 355:284-286(2017).
CC -!- FUNCTION: Peptide hormone required for contiguous Casparian strip
CC diffusion barrier formation in roots via the regulation of CASPs
CC protein expression and distribution in a GSO1-GSO2 signaling pathway.
CC The Casparian strip is required for ion homeostasis (e.g. iron and
CC potassium ions). {ECO:0000269|PubMed:28104889}.
CC -!- SUBUNIT: Interacts with the specific receptor kinases GSO1 and GSO2.
CC {ECO:0000269|PubMed:28104889}.
CC -!- TISSUE SPECIFICITY: Expressed exclusively in the root stele.
CC {ECO:0000269|PubMed:28104889}.
CC -!- DEVELOPMENTAL STAGE: Accumulates in the stele, especially at the phloem
CC pole, of the mature region of the primary roots.
CC {ECO:0000269|PubMed:28104889}.
CC -!- INDUCTION: Induced by excess iron and further synergistically regulated
CC by lowering the medium pH. {ECO:0000269|PubMed:28104889}.
CC -!- DISRUPTION PHENOTYPE: No detectable phenotype in cif1-1. The double
CC mutant cif1-1 cif2-1 is defective in ion homeostasis in the xylem due
CC to defect in endodermal barrier formation in the roots. Highly
CC sensitive to excess iron. Retarded growth under low-potassium
CC conditions. Repeatedly interrupted, discontinuous Casparian strip due
CC to patch-like localization of the CASPs proteins. Reduced rosette leaf
CC size. {ECO:0000269|PubMed:28104889}.
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DR EMBL; AC007047; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CP002685; AEC06491.1; -; Genomic_DNA.
DR EMBL; BT006387; AAP21195.1; -; mRNA.
DR EMBL; AK221226; BAD93812.1; -; mRNA.
DR EMBL; AK227669; BAE99656.1; -; mRNA.
DR RefSeq; NP_973472.1; NM_201743.3.
DR AlphaFoldDB; Q84MD2; -.
DR SMR; Q84MD2; -.
DR STRING; 3702.AT2G16385.1; -.
DR PaxDb; Q84MD2; -.
DR PRIDE; Q84MD2; -.
DR EnsemblPlants; AT2G16385.1; AT2G16385.1; AT2G16385.
DR GeneID; 2745542; -.
DR Gramene; AT2G16385.1; AT2G16385.1; AT2G16385.
DR KEGG; ath:AT2G16385; -.
DR Araport; AT2G16385; -.
DR TAIR; locus:1006230017; AT2G16385.
DR HOGENOM; CLU_173636_2_0_1; -.
DR InParanoid; Q84MD2; -.
DR OMA; MVERSMH; -.
DR OrthoDB; 1646713at2759; -.
DR PhylomeDB; Q84MD2; -.
DR PRO; PR:Q84MD2; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q84MD2; baseline and differential.
DR GO; GO:0048226; C:Casparian strip; IMP:UniProtKB.
DR GO; GO:0005179; F:hormone activity; IEA:UniProtKB-KW.
DR GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR GO; GO:0050801; P:ion homeostasis; IMP:UniProtKB.
DR GO; GO:0055072; P:iron ion homeostasis; IMP:UniProtKB.
DR GO; GO:2000067; P:regulation of root morphogenesis; IMP:UniProtKB.
DR GO; GO:0010447; P:response to acidic pH; IEP:UniProtKB.
DR GO; GO:0010039; P:response to iron ion; IEP:UniProtKB.
DR InterPro; IPR038974; CIF1/2.
DR PANTHER; PTHR35290; PTHR35290; 1.
PE 1: Evidence at protein level;
KW Cell wall biogenesis/degradation; Developmental protein; Hormone;
KW Hydroxylation; Reference proteome; Signal; Sulfation.
FT SIGNAL 1..22
FT /evidence="ECO:0000255"
FT CHAIN 23..83
FT /note="Protein CASPARIAN STRIP INTEGRITY FACTOR 1"
FT /id="PRO_5009346703"
FT REGION 59..83
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 64
FT /note="Sulfotyrosine"
FT /evidence="ECO:0000269|PubMed:28104889"
FT MOD_RES 69
FT /note="Hydroxyproline"
FT /evidence="ECO:0000269|PubMed:28104889"
FT MOD_RES 71
FT /note="Hydroxyproline"
FT /evidence="ECO:0000269|PubMed:28104889"
SQ SEQUENCE 83 AA; 9512 MW; F55C6F4AFD78B9AE CRC64;
MGMSPLTVKK LGFIFMIVSA SALSVSFAGR PSIFVHKKIN LREEMVERSM HEHERLLRMN
TKDYGNNSPS PRLERPPFKL IPN
