CIF2_ARATH
ID CIF2_ARATH Reviewed; 83 AA.
AC O65684;
DT 12-APR-2017, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1998, sequence version 1.
DT 03-AUG-2022, entry version 134.
DE RecName: Full=Protein CASPARIAN STRIP INTEGRITY FACTOR 2 {ECO:0000303|PubMed:28104889};
DE Flags: Precursor;
GN Name=CIF2 {ECO:0000303|PubMed:28104889};
GN OrderedLocusNames=At4g34600 {ECO:0000312|Araport:AT4G34600};
GN ORFNames=T4L20.180 {ECO:0000312|EMBL:CAA18839.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Cheuk R.F., Chen H., Kim C.J., Shinn P., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE,
RP INTERACTION WITH GSO1 AND GSO2, AND INDUCTION BY IRON.
RX PubMed=28104889; DOI=10.1126/science.aai9057;
RA Nakayama T., Shinohara H., Tanaka M., Baba K., Ogawa-Ohnishi M.,
RA Matsubayashi Y.;
RT "A peptide hormone required for Casparian strip diffusion barrier formation
RT in Arabidopsis roots.";
RL Science 355:284-286(2017).
CC -!- FUNCTION: Peptide hormone required for contiguous Casparian strip
CC diffusion barrier formation in roots via the regulation of CASPs
CC protein expression and distribution in a GSO1-GSO2 signaling pathway.
CC The Casparian strip is required for ion homeostasis (e.g. iron and
CC potassium ions). {ECO:0000269|PubMed:28104889}.
CC -!- SUBUNIT: Interacts with the specific receptor kinases GSO1 and GSO2.
CC {ECO:0000269|PubMed:28104889}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=Additional isoforms seem to exist.
CC {ECO:0000312|Araport:AT4G34600};
CC Name=1;
CC IsoId=O65684-1; Sequence=Displayed;
CC -!- TISSUE SPECIFICITY: Expressed exclusively in the root stele.
CC {ECO:0000269|PubMed:28104889}.
CC -!- DEVELOPMENTAL STAGE: Confined to the root stele in the elongation and
CC differentiation zones of both primary and lateral roots. Disappears at
CC the sites of lateral root initiation. In the root tip, present at and
CC above approximately 10 cells after the onset of elongation.
CC {ECO:0000269|PubMed:28104889}.
CC -!- INDUCTION: Induced by excess iron and further synergistically regulated
CC by lowering the medium pH. {ECO:0000269|PubMed:28104889}.
CC -!- DISRUPTION PHENOTYPE: Abnormal endodermal barrier formation in cif2-1.
CC The double mutant cif1-1 cif2-1 is defective in ion homeostasis in the
CC xylem due to defect in endodermal barrier formation in the roots.
CC Highly sensitive to excess iron. Retarded growth under low-potassium
CC conditions. Repeatedly interrupted, discontinuous Casparian strip due
CC to patch-like localization of the CASPs proteins. Reduced rosette leaf
CC size. {ECO:0000269|PubMed:28104889}.
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DR EMBL; AL023094; CAA18839.1; -; Genomic_DNA.
DR EMBL; AL161585; CAB80177.1; -; Genomic_DNA.
DR EMBL; CP002687; AEE86398.1; -; Genomic_DNA.
DR EMBL; BT010809; AAR24176.1; -; mRNA.
DR EMBL; BT014676; AAT35234.1; -; mRNA.
DR PIR; T05280; T05280.
DR RefSeq; NP_195186.1; NM_119626.4. [O65684-1]
DR PDB; 6S6Q; X-ray; 2.95 A; C/D=63-83.
DR PDBsum; 6S6Q; -.
DR AlphaFoldDB; O65684; -.
DR SMR; O65684; -.
DR STRING; 3702.AT4G34600.1; -.
DR PaxDb; O65684; -.
DR PRIDE; O65684; -.
DR ProteomicsDB; 246910; -. [O65684-1]
DR EnsemblPlants; AT4G34600.1; AT4G34600.1; AT4G34600. [O65684-1]
DR GeneID; 829612; -.
DR Gramene; AT4G34600.1; AT4G34600.1; AT4G34600. [O65684-1]
DR KEGG; ath:AT4G34600; -.
DR Araport; AT4G34600; -.
DR TAIR; locus:2139604; AT4G34600.
DR HOGENOM; CLU_173636_2_0_1; -.
DR InParanoid; O65684; -.
DR OMA; VHEHERI; -.
DR OrthoDB; 1646713at2759; -.
DR PhylomeDB; O65684; -.
DR PRO; PR:O65684; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; O65684; baseline and differential.
DR GO; GO:0048226; C:Casparian strip; IMP:UniProtKB.
DR GO; GO:0005179; F:hormone activity; IEA:UniProtKB-KW.
DR GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR GO; GO:0050801; P:ion homeostasis; IMP:UniProtKB.
DR GO; GO:0055072; P:iron ion homeostasis; IMP:UniProtKB.
DR GO; GO:0009860; P:pollen tube growth; IEP:TAIR.
DR GO; GO:2000067; P:regulation of root morphogenesis; IMP:UniProtKB.
DR GO; GO:0010447; P:response to acidic pH; IEP:UniProtKB.
DR GO; GO:0010039; P:response to iron ion; IEP:UniProtKB.
DR InterPro; IPR038974; CIF1/2.
DR PANTHER; PTHR35290; PTHR35290; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Alternative splicing; Cell wall biogenesis/degradation;
KW Developmental protein; Hormone; Hydroxylation; Reference proteome; Signal;
KW Sulfation.
FT SIGNAL 1..28
FT /evidence="ECO:0000255"
FT CHAIN 29..83
FT /note="Protein CASPARIAN STRIP INTEGRITY FACTOR 2"
FT /id="PRO_5009340946"
FT REGION 61..83
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 64
FT /note="Sulfotyrosine"
FT /evidence="ECO:0000250|UniProtKB:Q84MD2"
FT MOD_RES 69
FT /note="Hydroxyproline"
FT /evidence="ECO:0000250|UniProtKB:Q84MD2"
FT MOD_RES 71
FT /note="Hydroxyproline"
FT /evidence="ECO:0000250|UniProtKB:Q84MD2"
SQ SEQUENCE 83 AA; 9437 MW; 6AA23972D835DA13 CRC64;
MGLLPLVKKL GFIIFLLVSA SAFALCSAGR SSILIYSQED DHPEVVERRI HEHERILRMN
SRDYGHSSPK PKLVRPPFKL IPN
