CIK1_YEAST
ID CIK1_YEAST Reviewed; 594 AA.
AC Q01649; D6W023;
DT 01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-1993, sequence version 1.
DT 03-AUG-2022, entry version 159.
DE RecName: Full=Spindle pole body-associated protein CIK1;
DE AltName: Full=Chromosome instability and karyogamy protein 1;
GN Name=CIK1; OrderedLocusNames=YMR198W; ORFNames=YM9646.11;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=1644287; DOI=10.1101/gad.6.8.1414;
RA Page B.D., Snyder M.;
RT "CIK1: a developmentally regulated spindle pole body-associated protein
RT important for microtubule functions in Saccharomyces cerevisiae.";
RL Genes Dev. 6:1414-1429(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169872;
RA Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T.,
RA Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K.,
RA Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P.,
RA Skelton J., Walsh S.V., Whitehead S., Barrell B.G.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII.";
RL Nature 387:90-93(1997).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP FUNCTION, INTERACTION WITH KAR3, SUBCELLULAR LOCATION, AND INDUCTION.
RX PubMed=10087265; DOI=10.1083/jcb.144.6.1219;
RA Manning B.D., Barrett J.G., Wallace J.A., Granok H., Snyder M.;
RT "Differential regulation of the Kar3p kinesin-related protein by two
RT associated proteins, Cik1p and Vik1p.";
RL J. Cell Biol. 144:1219-1233(1999).
RN [5]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=11729143; DOI=10.1093/genetics/159.3.939;
RA Shanks R.M.Q., Kamieniecki R.J., Dawson D.S.;
RT "The Kar3-interacting protein Cik1p plays a critical role in passage
RT through meiosis I in Saccharomyces cerevisiae.";
RL Genetics 159:939-951(2001).
RN [6]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
CC -!- FUNCTION: A developmentally regulated protein important for microtubule
CC functions. Targets KAR3 to the cytoplasmic microtubules during mating.
CC Essential for KAR3 function during prophase of meiosis I. Required for
CC interhomolog recombination, synapsis of homologous chromosomes and
CC establishment of a meiosis I spindle. {ECO:0000269|PubMed:10087265,
CC ECO:0000269|PubMed:11729143}.
CC -!- SUBUNIT: Interacts with KAR3. {ECO:0000269|PubMed:10087265}.
CC -!- SUBCELLULAR LOCATION: Nucleus. Cytoplasm, cytoskeleton, microtubule
CC organizing center, spindle pole body.
CC -!- INDUCTION: By alpha factor. {ECO:0000269|PubMed:10087265}.
CC -!- MISCELLANEOUS: Present with 98 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
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DR EMBL; M96439; AAA34494.1; -; Genomic_DNA.
DR EMBL; Z47815; CAA87820.1; -; Genomic_DNA.
DR EMBL; BK006946; DAA10097.1; -; Genomic_DNA.
DR PIR; A44073; A44073.
DR RefSeq; NP_013925.1; NM_001182705.1.
DR AlphaFoldDB; Q01649; -.
DR SMR; Q01649; -.
DR BioGRID; 35376; 498.
DR DIP; DIP-192N; -.
DR IntAct; Q01649; 8.
DR MINT; Q01649; -.
DR STRING; 4932.YMR198W; -.
DR iPTMnet; Q01649; -.
DR MaxQB; Q01649; -.
DR PaxDb; Q01649; -.
DR PRIDE; Q01649; -.
DR EnsemblFungi; YMR198W_mRNA; YMR198W; YMR198W.
DR GeneID; 855238; -.
DR KEGG; sce:YMR198W; -.
DR SGD; S000004811; CIK1.
DR VEuPathDB; FungiDB:YMR198W; -.
DR eggNOG; ENOG502RIY9; Eukaryota.
DR GeneTree; ENSGT00940000176415; -.
DR HOGENOM; CLU_025801_0_0_1; -.
DR InParanoid; Q01649; -.
DR OMA; CFAYANE; -.
DR BioCyc; YEAST:G3O-32885-MON; -.
DR PRO; PR:Q01649; -.
DR Proteomes; UP000002311; Chromosome XIII.
DR RNAct; Q01649; protein.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR GO; GO:0005871; C:kinesin complex; IDA:SGD.
DR GO; GO:0005874; C:microtubule; IEA:UniProtKB-KW.
DR GO; GO:0005634; C:nucleus; HDA:SGD.
DR GO; GO:0005819; C:spindle; IDA:SGD.
DR GO; GO:0005816; C:spindle pole body; IDA:SGD.
DR GO; GO:0008017; F:microtubule binding; IEA:InterPro.
DR GO; GO:0003777; F:microtubule motor activity; IMP:SGD.
DR GO; GO:0000742; P:karyogamy involved in conjugation with cellular fusion; IMP:SGD.
DR GO; GO:0051321; P:meiotic cell cycle; IDA:SGD.
DR GO; GO:0000743; P:nuclear migration involved in conjugation with cellular fusion; IMP:SGD.
DR GO; GO:0060236; P:regulation of mitotic spindle organization; IMP:SGD.
DR InterPro; IPR031852; Vik1/Cik1_MT-bd.
DR Pfam; PF16796; Microtub_bd; 1.
PE 1: Evidence at protein level;
KW Coiled coil; Cytoplasm; Cytoskeleton; Microtubule; Nucleus;
KW Reference proteome.
FT CHAIN 1..594
FT /note="Spindle pole body-associated protein CIK1"
FT /id="PRO_0000089750"
FT REGION 1..29
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 81..360
FT /evidence="ECO:0000255"
FT COMPBIAS 1..18
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 594 AA; 69070 MW; A2A09DEB8C2EC838 CRC64;
MNNSKIPKLS FHSDPNNVTR DFPKTKRQKV QKREMDMILT PNNNKLNILH SSGSGIRRCY
TDDTSATYTK KLTFGGDPKI IERVKNNERK VRKDIDSLLN AISEIEKESV RIHARELPAI
TLELDAKVKA CRELQNEIDG LSTEMDLKDN QCDLQRKNVE LSSKNIVSMH AVKVQEFEND
LEEELSNAKR EWTYKLMEVE NLKPDERLTD EMRQLKTEFE EVNRKLFILQ NENENECKNY
KKELDKKFEI FKKVKNDARI ELDGEQERLS KVLKDLQDTH GELKENIKTC RDEFNDFEKR
IGEAEVNFHS MELAVVPLKK KLASTSQALT QVQEEKKQVE GEANNWKKKY VNELEKVQQE
LYTRQNLATS IEEIKGYTRC FAYANERQMP DEFHINYVDR CICENSGEKR VQVFDRVVLE
EIHKDHKRLY NECIPFLEKY ISKLINCSII VVSQQPTAPM KKTLLKQLIE QYGENYKMTL
NILHLDGSIK HSDVGLDNPT EIRDLSQDEE CMNILTLDTK LGKDEESHSM NIYIGSMSTV
QLNRELDDAP SVLSHILTKT KQCFVFKINA GENIEKALAL AGKLKRTITL PQLD
