ACFD_VIBC3
ID ACFD_VIBC3 Reviewed; 1520 AA.
AC A5F372; A5F371; C3LYL0; Q53270; Q56593; Q56615; Q56616; Q56617; Q57522;
AC Q9KTQ4;
DT 18-MAR-2008, integrated into UniProtKB/Swiss-Prot.
DT 18-MAR-2008, sequence version 2.
DT 25-MAY-2022, entry version 68.
DE RecName: Full=Accessory colonization factor AcfD;
DE Flags: Precursor;
GN Name=acfD; OrderedLocusNames=VC0395_A0369/VC0395_A0370, VC395_0861;
OS Vibrio cholerae serotype O1 (strain ATCC 39541 / Classical Ogawa 395 /
OS O395).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC Vibrio.
OX NCBI_TaxID=345073;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=11179381; DOI=10.1128/iai.69.3.1947-1952.2001;
RA Karaolis D.K.R., Lan R., Kaper J.B., Reeves P.R.;
RT "Comparison of Vibrio cholerae pathogenicity islands in sixth and seventh
RT pandemic strains.";
RL Infect. Immun. 69:1947-1952(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Kovach M.E.;
RL Thesis (1995), Louisana State University, United States.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 39541 / Classical Ogawa 395 / O395;
RA Heidelberg J.;
RL Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 39541 / Classical Ogawa 395 / O395;
RX PubMed=19115014; DOI=10.1371/journal.pone.0004053;
RA Feng L., Reeves P.R., Lan R., Ren Y., Gao C., Zhou Z., Ren Y., Cheng J.,
RA Wang W., Wang J., Qian W., Li D., Wang L.;
RT "A recalibrated molecular clock and independent origins for the cholera
RT pandemic clones.";
RL PLoS ONE 3:E4053-E4053(2008).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-383, AND FUNCTION.
RX PubMed=8063108; DOI=10.1016/0378-1119(94)90836-2;
RA Hughes K.J., Everiss K.D., Kovach M.E., Peterson K.M.;
RT "Sequence analysis of the Vibrio cholerae acfD gene reveals the presence of
RT an overlapping reading frame, orfZ, which encodes a protein that shares
RT sequence similarity to the FliA and FliC products of Salmonella.";
RL Gene 146:79-82(1994).
RN [6]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-52.
RX PubMed=7737517; DOI=10.1016/0378-1119(95)00054-a;
RA Hughes K.J., Everiss K.D., Kovach M.E., Peterson K.M.;
RT "Isolation and characterization of the Vibrio cholerae acfA gene, required
RT for efficient intestinal colonization.";
RL Gene 156:59-61(1995).
CC -!- FUNCTION: Disruption in Ogawa 395 results in decreased cell motility.
CC {ECO:0000269|PubMed:8063108}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000255|PROSITE-
CC ProRule:PRU00303}; Lipid-anchor {ECO:0000255|PROSITE-ProRule:PRU00303}.
CC -!- CAUTION: This protein is 88 amino acids long in strain Classical Ogawa
CC 395. However, there is a putative ribosomal frameshift motif present in
CC this strain which under special conditions might allow the full-length
CC protein to be translated. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAA64916.1; Type=Frameshift; Evidence={ECO:0000305};
CC Sequence=AAA64917.1; Type=Frameshift; Evidence={ECO:0000305};
CC Sequence=AAA82710.1; Type=Miscellaneous discrepancy; Note=In 4 pieces in Classical Ogawa 395. In 2 pieces in submission AAK20772.1 (same group, same strain). If frameshifted around position 82 reconstitutes the protein.; Evidence={ECO:0000305};
CC Sequence=AAA82711.1; Type=Miscellaneous discrepancy; Note=See above.; Evidence={ECO:0000305};
CC Sequence=AAA82712.1; Type=Miscellaneous discrepancy; Note=See above.; Evidence={ECO:0000305};
CC Sequence=AAA82713.1; Type=Miscellaneous discrepancy; Note=See above.; Evidence={ECO:0000305};
CC Sequence=AAK20772.1; Type=Frameshift; Evidence={ECO:0000305};
CC Sequence=ABQ19805.1; Type=Frameshift; Evidence={ECO:0000305};
CC Sequence=ABQ21840.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=ABQ21840.1; Type=Frameshift; Evidence={ECO:0000305};
CC Sequence=ACP08876.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AF325733; AAK20772.1; ALT_FRAME; Genomic_DNA.
DR EMBL; U39068; AAA82710.1; ALT_SEQ; Genomic_DNA.
DR EMBL; U39068; AAA82711.1; ALT_SEQ; Genomic_DNA.
DR EMBL; U39068; AAA82712.1; ALT_SEQ; Genomic_DNA.
DR EMBL; U39068; AAA82713.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP000627; ABQ19805.1; ALT_FRAME; Genomic_DNA.
DR EMBL; CP000627; ABQ21840.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP001235; ACP08876.1; ALT_FRAME; Genomic_DNA.
DR EMBL; U02373; AAA64916.1; ALT_FRAME; Genomic_DNA.
DR EMBL; U02373; AAA64917.1; ALT_FRAME; Genomic_DNA.
DR EMBL; L29060; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR PIR; A42962; A42962.
DR AlphaFoldDB; A5F372; -.
DR STRING; 345073.VC395_0861; -.
DR MEROPS; M98.001; -.
DR EnsemblBacteria; ABQ19805; ABQ19805; VC0395_A0370.
DR EnsemblBacteria; ABQ21840; ABQ21840; VC0395_A0369.
DR KEGG; vco:VC0395_A0369; -.
DR KEGG; vco:VC0395_A0370; -.
DR KEGG; vcr:VC395_0861; -.
DR PATRIC; fig|345073.21.peg.833; -.
DR eggNOG; COG3064; Bacteria.
DR HOGENOM; CLU_006312_0_0_6; -.
DR Proteomes; UP000000249; Chromosome 2.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR Gene3D; 1.10.390.30; -; 1.
DR InterPro; IPR025385; DUF4092.
DR InterPro; IPR035423; M60-like_N.
DR InterPro; IPR042279; Pep_M60_3.
DR InterPro; IPR031161; Peptidase_M60_dom.
DR Pfam; PF13322; DUF4092; 1.
DR Pfam; PF17291; M60-like_N; 1.
DR Pfam; PF13402; Peptidase_M60; 1.
DR SMART; SM01276; M60-like; 1.
DR PROSITE; PS51723; PEPTIDASE_M60; 1.
DR PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE 3: Inferred from homology;
KW Cell membrane; Lipoprotein; Membrane; Palmitate; Signal; Virulence.
FT SIGNAL 1..16
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT CHAIN 17..1520
FT /note="Accessory colonization factor AcfD"
FT /id="PRO_0000323014"
FT DOMAIN 1085..1388
FT /note="Peptidase M60"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01060"
FT LIPID 17
FT /note="N-palmitoyl cysteine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT LIPID 17
FT /note="S-diacylglycerol cysteine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT CONFLICT 138
FT /note="L -> I (in Ref. 2; AAA82711 and 5; AAA64917)"
FT /evidence="ECO:0000305"
FT CONFLICT 729
FT /note="V -> E (in Ref. 2; AAA82712)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1520 AA; 167832 MW; 0C6982ACAE83DB4A CRC64;
MKIRIVSLIV LGFLIGCKHE SIITPTVPAD GSGGNALNPG LVGGYLPDIG VPDPIISLSM
TLDGNLKFDS SLLCNDQDAS HFQISQKDNV FCTINGRSIA TFTAPFDANK NGRNTDSEVL
SLISADEYRD SPVRQENLQI LMKNMATIHG DKISLVFRST LDALTFENYL RHNLDLPKDQ
FLEAITEKIA NDNQVDKQPS THVPNISPSF TPGTSSNLNS PFVSANAEES LSYIPTDVIP
SLGRLLDSQG RVINGVSYFS NNTRGITGVD KTGAILNDGS FEFSWGDIIS FSIDTFELGS
TRANKTDFYI SELGKDNEGK NAEALIHRYA SIDDSKLIIP DKVTQIFSLY PNVINEVISL
SLPNGDIELD IGDGKTQIVP GEFFKQFDSG LAALIDQSIS PISRFKFEDS LPKKKSAIDS
ESSQIQDIIN KLWGATDTVQ ANGWKKVDRF HIFHDSTNFY GSTGSARAQA AVNIANSAFP
VLMARNDNNY WIDFGKPKAW DSNSLAFITE APSTVVPDKV SEDTSTFNLP FISLGEIGKG
KLMVLGNARY NSVLVCPNGF SWGGTVKNGT CSLSSDRDDM ANFFSNVIRY LTGSTSNDVI
VGTNIPEVYF KSSGQTMGSK ANFELDSRFS KQTQQLTSFH DLDVNTIPLI IINAYDYKGK
NINSPYDIPL SADVGSPKLS RSDVTDLIDY INNGGSVLMM ETIINTNNSE ISRLLDSAGI
AFGIGNSVVA DGNGPSGGHP DRPRSQREHG IWVIERYAAV EDESSGQQTL PYVINSDGSI
EWKYIVENRP DDKPKLEVAS WVESEAGDKL ITHYAFIDES QHWKKDISGK IIYNVAGKPE
VDNASLSLAK NKVLDAFKNS SGQRAYSECK NSEFHYEINC LEYRPGNSIP ITGGLYVPRY
TDIKLGESEA NAMVKAANLG TNIHALYQHE RYFRTKGKSG ARLNSVDLNR IYQNMSVWLW
NDLDYRYDDK QSDELGFKVF TQYLNCYTSN NAGGNTTCPE ELKDELTQLG MIYDEKSGSY
AGQMDPSYPL NYMEKPLTRL MLGRSFWDLD IKVDVRKYPG EVTTRSGGGD ITLDMRNNTA
AWFAGNRQPT GQWAEAHQPF SVSVSGETSP VTITIALADD LTGREKHELG LKRPPRMSKS
FVIGGDSPKM QTFTVPYGGL IYAQGGNSQQ VKLTFSGTID APLYIDGKWR NPLLSGAPIG
EVVSDTFIFT APKANLNADG YLGGIEQFAK DLDQFSADLN DFYARDEGAD GDKNRKATDK
SMPNNRHHFV NDVAISVGAA HSGYPVMNDS FITSSRSLNT MPLNSWLLWH EVGHNSAEAP
FNVDGATEVV NNLLALYMQD RHQGKMSRVE QDIRYAFDFV NAEHGHAWGA GGAGERLVMF
AQLKEWAETE FDINDWYNDK LPGFYIEESG IKGWNLFKLM HRLMRNENDD QINMKGENQC
KISGIGKSDL LMLCASYAAQ TDLSEFFKAW NPGSKAFLYP DDPQPYYEGG ITPSGIQRVK
SLKLNLPQKN PLSINSVTQH
