ID   CILA_LEUMC              Reviewed;         512 AA.
AC   O53079;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1998, sequence version 1.
DT   25-MAY-2022, entry version 64.
DE   RecName: Full=Citrate lyase alpha chain;
DE            Short=Citrase alpha chain;
DE            EC=4.1.3.6;
DE   AltName: Full=Citrate (pro-3S)-lyase alpha chain;
DE   AltName: Full=Citrate CoA-transferase subunit;
DE            EC=2.8.3.10;
GN   Name=citF;
OS   Leuconostoc mesenteroides subsp. cremoris.
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae;
OC   Leuconostoc.
OX   NCBI_TaxID=33965;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=195;
RX   PubMed=9457870; DOI=10.1128/jb.180.3.647-654.1998;
RA   Bekal S., van Beeumen J., Samyn B., Garmyn D., Henini S., Divies C.,
RA   Prevost H.;
RT   "Purification of Leuconostoc mesenteroides citrate lyase and cloning and
RT   characterization of the citCDEFG gene cluster.";
RL   J. Bacteriol. 180:647-654(1998).
CC   -!- FUNCTION: Represents a citrate:acetyl-ACP transferase. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=citrate = acetate + oxaloacetate; Xref=Rhea:RHEA:10760,
CC         ChEBI:CHEBI:16452, ChEBI:CHEBI:16947, ChEBI:CHEBI:30089; EC=4.1.3.6;
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=acetyl-CoA + citrate = (3S)-citryl-CoA + acetate;
CC         Xref=Rhea:RHEA:19405, ChEBI:CHEBI:16947, ChEBI:CHEBI:30089,
CC         ChEBI:CHEBI:57288, ChEBI:CHEBI:57321; EC=2.8.3.10;
CC   -!- SUBUNIT: Oligomer with a subunit composition of (alpha,beta,gamma)6.
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
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DR   EMBL; Y10621; CAA71633.1; -; Genomic_DNA.
DR   RefSeq; WP_050892380.1; NZ_LAYV01000030.1.
DR   AlphaFoldDB; O53079; -.
DR   SMR; O53079; -.
DR   PATRIC; fig|33965.3.peg.1360; -.
DR   GO; GO:0009346; C:ATP-independent citrate lyase complex; IEA:InterPro.
DR   GO; GO:0008815; F:citrate (pro-3S)-lyase activity; IEA:UniProtKB-EC.
DR   GO; GO:0008814; F:citrate CoA-transferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006084; P:acetyl-CoA metabolic process; IEA:InterPro.
DR   InterPro; IPR006472; Citrate_lyase_asu.
DR   InterPro; IPR037171; NagB/RpiA_transferase-like.
DR   PANTHER; PTHR40596; PTHR40596; 1.
DR   Pfam; PF04223; CitF; 1.
DR   PIRSF; PIRSF009451; Citrt_lyas_alpha; 1.
DR   SUPFAM; SSF100950; SSF100950; 2.
DR   TIGRFAMs; TIGR01584; citF; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Lyase; Transferase.
FT   CHAIN           1..512
FT                   /note="Citrate lyase alpha chain"
FT                   /id="PRO_0000089755"
SQ   SEQUENCE   512 AA;  55068 MW;  6617CBB96B5A73C0 CRC64;
     MKNKVNIDVP DAILEQLDDS VFESTNYGNP EIQRVGPKVR ATTGESKVQS SIDDVLSNTL
     KDGMTISFHH HFREGDFVFN KVMRKIIDMG YQNLTLAPSS LTNVMNDIVI EAIKKGVVTN
     ITSSGMRGTL GDAVSHGILK NPVIFRSHGA RARAIESGEI KIDVAFLGVP NSDEMGNANG
     MNGDAAFGSL GYALIDAQYA DKLVLITDTI MPYPNTPASI KQTQVDYVVK VDKVGDPDKI
     GSGATRFTKD PKELKIAKTV NDVIVNSKYF KNDFSFQTGS GGAALAVTRF LREAMMAQNI
     MASFALGGIT KPTVDLLNEG LVNRVMDVQD FDKGAASSMK LSPNQQEIDA SWYADPANKG
     AMVDKLDVAI LSALEVDTNF NVNVMSGSDG VIRGAIGGHQ DAATAKLTII SVPLVRGRIA
     TIVPKVNTVI TPGDSIDVVV TEVGIAINPK RTDLVEQLKQ VPGLPIYSIE ELQQKAEKIV
     GQPAPLKFTD RVVAVAEYRD GSVIDIIKEV AD