ID   ACFD_VIBCH              Reviewed;        1520 AA.
AC   P0C6F0; Q53270; Q56593; Q56615; Q56616; Q56617; Q57522; Q9KTQ4;
DT   18-MAR-2008, integrated into UniProtKB/Swiss-Prot.
DT   18-MAR-2008, sequence version 1.
DT   03-AUG-2022, entry version 68.
DE   RecName: Full=Accessory colonization factor AcfD;
DE   Flags: Precursor;
GN   Name=acfD; OrderedLocusNames=VC_0845;
OS   Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC   Vibrio.
OX   NCBI_TaxID=243277;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 39315 / El Tor Inaba N16961;
RX   PubMed=11179381; DOI=10.1128/iai.69.3.1947-1952.2001;
RA   Karaolis D.K.R., Lan R., Kaper J.B., Reeves P.R.;
RT   "Comparison of Vibrio cholerae pathogenicity islands in sixth and seventh
RT   pandemic strains.";
RL   Infect. Immun. 69:1947-1952(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 39315 / El Tor Inaba N16961;
RX   PubMed=10952301; DOI=10.1038/35020000;
RA   Heidelberg J.F., Eisen J.A., Nelson W.C., Clayton R.A., Gwinn M.L.,
RA   Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Umayam L.A., Gill S.R.,
RA   Nelson K.E., Read T.D., Tettelin H., Richardson D.L., Ermolaeva M.D.,
RA   Vamathevan J.J., Bass S., Qin H., Dragoi I., Sellers P., McDonald L.A.,
RA   Utterback T.R., Fleischmann R.D., Nierman W.C., White O., Salzberg S.L.,
RA   Smith H.O., Colwell R.R., Mekalanos J.J., Venter J.C., Fraser C.M.;
RT   "DNA sequence of both chromosomes of the cholera pathogen Vibrio
RT   cholerae.";
RL   Nature 406:477-483(2000).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-53.
RX   PubMed=1644747; DOI=10.1128/jb.174.16.5211-5218.1992;
RA   Parsot C., Mekalanos J.J.;
RT   "Structural analysis of the acfA and acfD genes of Vibrio cholerae: effects
RT   of DNA topology and transcriptional activators on expression.";
RL   J. Bacteriol. 174:5211-5218(1992).
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000255|PROSITE-
CC       ProRule:PRU00303}; Lipid-anchor {ECO:0000255|PROSITE-ProRule:PRU00303}.
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DR   EMBL; AF325734; AAK20802.1; -; Genomic_DNA.
DR   EMBL; AE003852; AAF94008.1; -; Genomic_DNA.
DR   EMBL; S41756; AAB22796.1; -; Genomic_DNA.
DR   PIR; A42962; A42962.
DR   PIR; B82274; B82274.
DR   RefSeq; NP_230493.1; NC_002505.1.
DR   RefSeq; WP_000702220.1; NZ_LT906614.1.
DR   AlphaFoldDB; P0C6F0; -.
DR   STRING; 243277.VC_0845; -.
DR   MEROPS; M98.001; -.
DR   EnsemblBacteria; AAF94008; AAF94008; VC_0845.
DR   GeneID; 57739547; -.
DR   KEGG; vch:VC_0845; -.
DR   PATRIC; fig|243277.26.peg.805; -.
DR   eggNOG; COG3064; Bacteria.
DR   HOGENOM; CLU_006312_0_0_6; -.
DR   OMA; VMGNARY; -.
DR   BioCyc; VCHO:VC0845-MON; -.
DR   Proteomes; UP000000584; Chromosome 1.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   Gene3D; 1.10.390.30; -; 1.
DR   InterPro; IPR025385; DUF4092.
DR   InterPro; IPR035423; M60-like_N.
DR   InterPro; IPR042279; Pep_M60_3.
DR   InterPro; IPR031161; Peptidase_M60_dom.
DR   Pfam; PF13322; DUF4092; 1.
DR   Pfam; PF17291; M60-like_N; 1.
DR   Pfam; PF13402; Peptidase_M60; 1.
DR   SMART; SM01276; M60-like; 1.
DR   PROSITE; PS51723; PEPTIDASE_M60; 1.
DR   PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE   3: Inferred from homology;
KW   Cell membrane; Lipoprotein; Membrane; Palmitate; Reference proteome;
KW   Signal; Virulence.
FT   SIGNAL          1..16
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT   CHAIN           17..1520
FT                   /note="Accessory colonization factor AcfD"
FT                   /id="PRO_0000020620"
FT   DOMAIN          1085..1388
FT                   /note="Peptidase M60"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01060"
FT   LIPID           17
FT                   /note="N-palmitoyl cysteine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT   LIPID           17
FT                   /note="S-diacylglycerol cysteine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT   CONFLICT        52..53
FT                   /note="PD -> RI (in Ref. 3; AAB22796)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   1520 AA;  167832 MW;  0C6982ACAE83DB4A CRC64;
     MKIRIVSLIV LGFLIGCKHE SIITPTVPAD GSGGNALNPG LVGGYLPDIG VPDPIISLSM
     TLDGNLKFDS SLLCNDQDAS HFQISQKDNV FCTINGRSIA TFTAPFDANK NGRNTDSEVL
     SLISADEYRD SPVRQENLQI LMKNMATIHG DKISLVFRST LDALTFENYL RHNLDLPKDQ
     FLEAITEKIA NDNQVDKQPS THVPNISPSF TPGTSSNLNS PFVSANAEES LSYIPTDVIP
     SLGRLLDSQG RVINGVSYFS NNTRGITGVD KTGAILNDGS FEFSWGDIIS FSIDTFELGS
     TRANKTDFYI SELGKDNEGK NAEALIHRYA SIDDSKLIIP DKVTQIFSLY PNVINEVISL
     SLPNGDIELD IGDGKTQIVP GEFFKQFDSG LAALIDQSIS PISRFKFEDS LPKKKSAIDS
     ESSQIQDIIN KLWGATDTVQ ANGWKKVDRF HIFHDSTNFY GSTGSARAQA AVNIANSAFP
     VLMARNDNNY WIDFGKPKAW DSNSLAFITE APSTVVPDKV SEDTSTFNLP FISLGEIGKG
     KLMVLGNARY NSVLVCPNGF SWGGTVKNGT CSLSSDRDDM ANFFSNVIRY LTGSTSNDVI
     VGTNIPEVYF KSSGQTMGSK ANFELDSRFS KQTQQLTSFH DLDVNTIPLI IINAYDYKGK
     NINSPYDIPL SADVGSPKLS RSDVTDLIDY INNGGSVLMM ETIINTNNSE ISRLLDSAGI
     AFGIGNSVVA DGNGPSGGHP DRPRSQREHG IWVIERYAAV EDESSGQQTL PYVINSDGSI
     EWKYIVENRP DDKPKLEVAS WVESEAGDKL ITHYAFIDES QHWKKDISGK IIYNVAGKPE
     VDNASLSLAK NKVLDAFKNS SGQRAYSECK NSEFHYEINC LEYRPGNSIP ITGGLYVPRY
     TDIKLGESEA NAMVKAANLG TNIHALYQHE RYFRTKGKSG ARLNSVDLNR IYQNMSVWLW
     NDLDYRYDDK QSDELGFKVF TQYLNCYTSN NAGGNTTCPE ELKDELTQLG MIYDEKSGSY
     AGQMDPSYPL NYMEKPLTRL MLGRSFWDLD IKVDVRKYPG EVTTRSGGGD ITLDMRNNTA
     AWFAGNRQPT GQWAEAHQPF SVSVSGETSP VTITIALADD LTGREKHELG LKRPPRMSKS
     FVIGGDSPKM QTFTVPYGGL IYAQGGNSQQ VKLTFSGTID APLYIDGKWR NPLLSGAPIG
     EVVSDTFIFT APKANLNADG YLGGIEQFAK DLDQFSADLN DFYARDEGAD GDKNRKATDK
     SMPNNRHHFV NDVAISVGAA HSGYPVMNDS FITSSRSLNT MPLNSWLLWH EVGHNSAEAP
     FNVDGATEVV NNLLALYMQD RHQGKMSRVE QDIRYAFDFV NAEHGHAWGA GGAGERLVMF
     AQLKEWAETE FDINDWYNDK LPGFYIEESG IKGWNLFKLM HRLMRNENDD QINMKGENQC
     KISGIGKSDL LMLCASYAAQ TDLSEFFKAW NPGSKAFLYP DDPQPYYEGG ITPSGIQRVK
     SLKLNLPQKN PLSINSVTQH