ID   CIMA_AQUAE              Reviewed;         528 AA.
AC   O66682;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1998, sequence version 1.
DT   03-AUG-2022, entry version 102.
DE   RecName: Full=(R)-citramalate synthase {ECO:0000305};
DE            EC=2.3.1.182 {ECO:0000250|UniProtKB:Q74C76};
GN   Name=cimA {ECO:0000305}; OrderedLocusNames=aq_356;
OS   Aquifex aeolicus (strain VF5).
OC   Bacteria; Aquificae; Aquificales; Aquificaceae; Aquifex.
OX   NCBI_TaxID=224324;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=VF5;
RX   PubMed=9537320; DOI=10.1038/32831;
RA   Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L.,
RA   Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R.,
RA   Feldman R.A., Short J.M., Olsen G.J., Swanson R.V.;
RT   "The complete genome of the hyperthermophilic bacterium Aquifex aeolicus.";
RL   Nature 392:353-358(1998).
CC   -!- FUNCTION: Catalyzes the condensation of pyruvate and acetyl-coenzyme A
CC       to form (R)-citramalate. {ECO:0000250|UniProtKB:Q74C76}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=acetyl-CoA + H2O + pyruvate = (3R)-citramalate + CoA + H(+);
CC         Xref=Rhea:RHEA:19045, ChEBI:CHEBI:15361, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:30934, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57288; EC=2.3.1.182;
CC         Evidence={ECO:0000250|UniProtKB:Q74C76};
CC   -!- PATHWAY: Amino-acid biosynthesis; L-isoleucine biosynthesis; 2-
CC       oxobutanoate from pyruvate: step 1/3. {ECO:0000250|UniProtKB:Q74C76}.
CC   -!- SIMILARITY: Belongs to the alpha-IPM synthase/homocitrate synthase
CC       family. {ECO:0000305}.
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DR   EMBL; AE000657; AAC06637.1; -; Genomic_DNA.
DR   PIR; F70331; F70331.
DR   RefSeq; NP_213242.1; NC_000918.1.
DR   RefSeq; WP_010880180.1; NC_000918.1.
DR   AlphaFoldDB; O66682; -.
DR   SMR; O66682; -.
DR   STRING; 224324.aq_356; -.
DR   EnsemblBacteria; AAC06637; AAC06637; aq_356.
DR   KEGG; aae:aq_356; -.
DR   PATRIC; fig|224324.8.peg.286; -.
DR   eggNOG; COG0119; Bacteria.
DR   HOGENOM; CLU_022158_7_0_0; -.
DR   InParanoid; O66682; -.
DR   OMA; KSWDFHV; -.
DR   OrthoDB; 840579at2; -.
DR   UniPathway; UPA00047; UER00066.
DR   Proteomes; UP000000798; Chromosome.
DR   GO; GO:0043714; F:(R)-citramalate synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0003852; F:2-isopropylmalate synthase activity; IEA:InterPro.
DR   GO; GO:0009097; P:isoleucine biosynthetic process; IEA:UniProtKB-UniPathway.
DR   GO; GO:0009098; P:leucine biosynthetic process; IEA:InterPro.
DR   Gene3D; 3.20.20.70; -; 1.
DR   Gene3D; 3.30.160.270; -; 1.
DR   InterPro; IPR013709; 2-isopropylmalate_synth_dimer.
DR   InterPro; IPR002034; AIPM/Hcit_synth_CS.
DR   InterPro; IPR013785; Aldolase_TIM.
DR   InterPro; IPR005675; Citramal_synthase.
DR   InterPro; IPR036230; LeuA_allosteric_dom_sf.
DR   InterPro; IPR000891; PYR_CT.
DR   PANTHER; PTHR43538; PTHR43538; 1.
DR   Pfam; PF00682; HMGL-like; 1.
DR   Pfam; PF08502; LeuA_dimer; 1.
DR   SMART; SM00917; LeuA_dimer; 1.
DR   SUPFAM; SSF110921; SSF110921; 1.
DR   TIGRFAMs; TIGR00977; citramal_synth; 1.
DR   PROSITE; PS00815; AIPM_HOMOCIT_SYNTH_1; 1.
DR   PROSITE; PS50991; PYR_CT; 1.
PE   3: Inferred from homology;
KW   Amino-acid biosynthesis; Branched-chain amino acid biosynthesis;
KW   Isoleucine biosynthesis; Reference proteome; Transferase.
FT   CHAIN           1..528
FT                   /note="(R)-citramalate synthase"
FT                   /id="PRO_0000140469"
FT   DOMAIN          5..271
FT                   /note="Pyruvate carboxyltransferase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01151"
SQ   SEQUENCE   528 AA;  59215 MW;  1BC6ABAEC35D3F34 CRC64;
     MAEKVYIYDT TLRDGSQMEG VSFSLEDKIR IAEKLDDFGI HYIEGGWPYA NPKDNLFFQK
     AKKMNFKNAK LTAFGSTRRP NKKVSEDPQV ESLIKAETPV VTIFGKSWDL HVTDALKTTL
     EENLNMIYET VEYLKRYVDE VIFDAEHFFD GYKSNPEYAL QVLEAALKGG ADWVVLCDTN
     GGTLPHEIYE ITKKVKERFK DANVGIHAHN DSETAVANSL MAVLAGARQV HGTINGIGER
     TGNANLCSII PNLQLKLGFD VIPQENLKKL TELANFVAEI INMPLPRNMP YVGESAFAHK
     GGVHASAVLK NAKTYEHINP ELVGNKRKIT VSDLAGRSNL VHKLKEFGIE IDPKSPELKK
     LIDKIKELEK EGYHFEAAEA SLELLIKRHF GLVKDYFDFD AYRVLIAKRR DDSLPTSEAT
     VRLSVEGVEE HTASLGNGPI SALDRALRKA LEEFYPNLKE LQLIDYKVRI INESAGTSAK
     VRVLIESTDG KRKWGTVGVS ENVIEASWIA LRDSIVYKLM KDEEEGIL