ID   CIMA_METPE              Reviewed;         500 AA.
AC   B8GEI1;
DT   03-MAY-2011, integrated into UniProtKB/Swiss-Prot.
DT   03-MAR-2009, sequence version 1.
DT   03-AUG-2022, entry version 64.
DE   RecName: Full=Putative (R)-citramalate synthase CimA {ECO:0000255|HAMAP-Rule:MF_01028};
DE            EC=2.3.1.182 {ECO:0000255|HAMAP-Rule:MF_01028};
GN   Name=cimA {ECO:0000255|HAMAP-Rule:MF_01028}; OrderedLocusNames=Mpal_2402;
OS   Methanosphaerula palustris (strain ATCC BAA-1556 / DSM 19958 / E1-9c).
OC   Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
OC   Methanomicrobiales; Methanoregulaceae; Methanosphaerula.
OX   NCBI_TaxID=521011;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-1556 / DSM 19958 / E1-9c;
RX   PubMed=26543115; DOI=10.1128/genomea.01280-15;
RA   Cadillo-Quiroz H., Browne P., Kyrpides N., Woyke T., Goodwin L., Detter C.,
RA   Yavitt J.B., Zinder S.H.;
RT   "Complete Genome Sequence of Methanosphaerula palustris E1-9CT, a
RT   Hydrogenotrophic Methanogen Isolated from a Minerotrophic Fen Peatland.";
RL   Genome Announc. 3:0-0(2015).
CC   -!- FUNCTION: Catalyzes the condensation of pyruvate and acetyl-coenzyme A
CC       to form (R)-citramalate. {ECO:0000255|HAMAP-Rule:MF_01028}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=acetyl-CoA + H2O + pyruvate = (3R)-citramalate + CoA + H(+);
CC         Xref=Rhea:RHEA:19045, ChEBI:CHEBI:15361, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:30934, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57288; EC=2.3.1.182; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_01028};
CC   -!- PATHWAY: Amino-acid biosynthesis; L-isoleucine biosynthesis; 2-
CC       oxobutanoate from pyruvate: step 1/3. {ECO:0000255|HAMAP-
CC       Rule:MF_01028}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000255|HAMAP-Rule:MF_01028}.
CC   -!- SIMILARITY: Belongs to the alpha-IPM synthase/homocitrate synthase
CC       family. {ECO:0000255|HAMAP-Rule:MF_01028}.
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DR   EMBL; CP001338; ACL17682.1; -; Genomic_DNA.
DR   RefSeq; WP_012619001.1; NC_011832.1.
DR   AlphaFoldDB; B8GEI1; -.
DR   SMR; B8GEI1; -.
DR   STRING; 521011.Mpal_2402; -.
DR   PRIDE; B8GEI1; -.
DR   EnsemblBacteria; ACL17682; ACL17682; Mpal_2402.
DR   GeneID; 7272125; -.
DR   KEGG; mpl:Mpal_2402; -.
DR   eggNOG; arCOG02092; Archaea.
DR   HOGENOM; CLU_022158_0_1_2; -.
DR   OMA; SNMFAHE; -.
DR   OrthoDB; 10632at2157; -.
DR   UniPathway; UPA00047; UER00066.
DR   Proteomes; UP000002457; Chromosome.
DR   GO; GO:0043714; F:(R)-citramalate synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0003852; F:2-isopropylmalate synthase activity; IEA:InterPro.
DR   GO; GO:0009097; P:isoleucine biosynthetic process; IEA:UniProtKB-UniRule.
DR   GO; GO:0009098; P:leucine biosynthetic process; IEA:InterPro.
DR   Gene3D; 3.20.20.70; -; 1.
DR   Gene3D; 3.30.160.270; -; 1.
DR   HAMAP; MF_01028; CimA; 1.
DR   InterPro; IPR013709; 2-isopropylmalate_synth_dimer.
DR   InterPro; IPR002034; AIPM/Hcit_synth_CS.
DR   InterPro; IPR013785; Aldolase_TIM.
DR   InterPro; IPR024890; Citramalate_synthase_CimA.
DR   InterPro; IPR011830; LEU1_arch.
DR   InterPro; IPR036230; LeuA_allosteric_dom_sf.
DR   InterPro; IPR000891; PYR_CT.
DR   Pfam; PF00682; HMGL-like; 1.
DR   Pfam; PF08502; LeuA_dimer; 1.
DR   SMART; SM00917; LeuA_dimer; 1.
DR   SUPFAM; SSF110921; SSF110921; 1.
DR   TIGRFAMs; TIGR02090; LEU1_arch; 1.
DR   PROSITE; PS00815; AIPM_HOMOCIT_SYNTH_1; 1.
DR   PROSITE; PS00816; AIPM_HOMOCIT_SYNTH_2; 1.
DR   PROSITE; PS50991; PYR_CT; 1.
PE   3: Inferred from homology;
KW   Amino-acid biosynthesis; Branched-chain amino acid biosynthesis;
KW   Isoleucine biosynthesis; Reference proteome; Transferase.
FT   CHAIN           1..500
FT                   /note="Putative (R)-citramalate synthase CimA"
FT                   /id="PRO_0000407914"
FT   DOMAIN          9..258
FT                   /note="Pyruvate carboxyltransferase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01151"
SQ   SEQUENCE   500 AA;  52466 MW;  EC816162A3D79657 CRC64;
     MIALFGEKLR FFDTTLRDGE QTPGVSLTPD QKCIIATALA AVGVDVIEAG SAAASPGDRA
     AIRQIANAGL GVEVCTFVRA MTGDIDAAVE CGVDSVHLVV PVSDLHIQKK LRKDRATVAA
     MANKAVDYAK ERGLIVELSG EDASRADQSF LAEVFASGVE HGADRLCFCD TVGLMTPERT
     ALMIPPLAAI APLSIHCHDD LGFGLATTIA ALKAGASCAH VTVNGLGERA GNTPLEEVVM
     ALERLYGYDT RIRTERLYPL SVLVSRLTGV PLPAQKPIVG ELAFTHESGI HADGILKDPS
     TYEPLAPETV GRTRRIILGK HSGSASVEAA LQESGYRPTP AQLQVIVARV KHLGDQGHRV
     TDGDLAAIAE AVLEVSTEPV LVLKQFTVVS GSGVLPTASV TLLVNGAEVT NAATGTGPVD
     AAIEALRRSV ADVAAISLLE YHVDAITGGT DALVEVTATL SNGEMTRTSR GAETDIIAAS
     VEAVVQGMNR LLRRRDEDRR