ID   CIMA_SYNY3              Reviewed;         547 AA.
AC   P74269;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1997, sequence version 1.
DT   25-MAY-2022, entry version 117.
DE   RecName: Full=(R)-citramalate synthase {ECO:0000305};
DE            EC=2.3.1.182 {ECO:0000250|UniProtKB:Q74C76};
GN   Name=cimA {ECO:0000305}; OrderedLocusNames=sll1564;
OS   Synechocystis sp. (strain PCC 6803 / Kazusa).
OC   Bacteria; Cyanobacteria; Synechococcales; Merismopediaceae; Synechocystis;
OC   unclassified Synechocystis.
OX   NCBI_TaxID=1111708;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=PCC 6803 / Kazusa;
RX   PubMed=8905231; DOI=10.1093/dnares/3.3.109;
RA   Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y.,
RA   Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T.,
RA   Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S.,
RA   Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT   "Sequence analysis of the genome of the unicellular cyanobacterium
RT   Synechocystis sp. strain PCC6803. II. Sequence determination of the entire
RT   genome and assignment of potential protein-coding regions.";
RL   DNA Res. 3:109-136(1996).
CC   -!- FUNCTION: Catalyzes the condensation of pyruvate and acetyl-coenzyme A
CC       to form (R)-citramalate. {ECO:0000250|UniProtKB:Q74C76}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=acetyl-CoA + H2O + pyruvate = (3R)-citramalate + CoA + H(+);
CC         Xref=Rhea:RHEA:19045, ChEBI:CHEBI:15361, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:30934, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57288; EC=2.3.1.182;
CC         Evidence={ECO:0000250|UniProtKB:Q74C76};
CC   -!- PATHWAY: Amino-acid biosynthesis; L-isoleucine biosynthesis; 2-
CC       oxobutanoate from pyruvate: step 1/3. {ECO:0000250|UniProtKB:Q74C76}.
CC   -!- SIMILARITY: Belongs to the alpha-IPM synthase/homocitrate synthase
CC       family. {ECO:0000305}.
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DR   EMBL; BA000022; BAA18363.1; -; Genomic_DNA.
DR   PIR; S75904; S75904.
DR   AlphaFoldDB; P74269; -.
DR   SMR; P74269; -.
DR   IntAct; P74269; 3.
DR   STRING; 1148.1653449; -.
DR   PaxDb; P74269; -.
DR   EnsemblBacteria; BAA18363; BAA18363; BAA18363.
DR   KEGG; syn:sll1564; -.
DR   eggNOG; COG0119; Bacteria.
DR   InParanoid; P74269; -.
DR   OMA; KSWDFHV; -.
DR   PhylomeDB; P74269; -.
DR   UniPathway; UPA00047; UER00066.
DR   Proteomes; UP000001425; Chromosome.
DR   GO; GO:0043714; F:(R)-citramalate synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0003852; F:2-isopropylmalate synthase activity; IEA:InterPro.
DR   GO; GO:0009097; P:isoleucine biosynthetic process; IEA:UniProtKB-UniPathway.
DR   GO; GO:0009098; P:leucine biosynthetic process; IEA:InterPro.
DR   Gene3D; 3.20.20.70; -; 1.
DR   Gene3D; 3.30.160.270; -; 1.
DR   InterPro; IPR013709; 2-isopropylmalate_synth_dimer.
DR   InterPro; IPR002034; AIPM/Hcit_synth_CS.
DR   InterPro; IPR013785; Aldolase_TIM.
DR   InterPro; IPR005675; Citramal_synthase.
DR   InterPro; IPR036230; LeuA_allosteric_dom_sf.
DR   InterPro; IPR000891; PYR_CT.
DR   PANTHER; PTHR43538; PTHR43538; 1.
DR   Pfam; PF00682; HMGL-like; 1.
DR   Pfam; PF08502; LeuA_dimer; 1.
DR   SMART; SM00917; LeuA_dimer; 1.
DR   SUPFAM; SSF110921; SSF110921; 1.
DR   TIGRFAMs; TIGR00977; citramal_synth; 1.
DR   PROSITE; PS00815; AIPM_HOMOCIT_SYNTH_1; 1.
DR   PROSITE; PS00816; AIPM_HOMOCIT_SYNTH_2; 1.
DR   PROSITE; PS50991; PYR_CT; 1.
PE   3: Inferred from homology;
KW   Amino-acid biosynthesis; Branched-chain amino acid biosynthesis;
KW   Isoleucine biosynthesis; Reference proteome; Transferase.
FT   CHAIN           1..547
FT                   /note="(R)-citramalate synthase"
FT                   /id="PRO_0000140471"
FT   DOMAIN          8..278
FT                   /note="Pyruvate carboxyltransferase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01151"
SQ   SEQUENCE   547 AA;  59820 MW;  9344A317735CAB47 CRC64;
     MATKKTSLWL YDTTLRDGAQ REGISLSLTD KLTIARRLDQ LGIPFIEGGW PGANPKDVQF
     FWQLQEEPLE QAEIVAFCST RRPHKAVETD KMLQAILSAG TRWVTIFGKS WDLHVLEGLQ
     TSLAENLAMI SDTIAYLRSQ GRRVIYDAEH WFDGYRANPD YALATLATAQ QAGAEWLVMC
     DTNGGTLPGQ ISEITTKVRR SLGLDGQSDR QPQLGIHAHN DSGTAVANSL LAVEAGATMV
     QGTINGYGER CGNANLCTLI PNLQLKLDYD CIEPEKLAHL TSTSRLISEI VNLAPDDHAP
     FVGRSAFAHK GGIHVSAVQR NPFTYEHIAP NLVGNERRIV VSEQAGLSNV LSKAELFGIA
     LDRQNPACRT ILATLKDLEQ QGYQFEAAEA SFELLMRQAM GDRQPLFLVQ GFQVHCDLLT
     PAENPAYRNA LATVKVTVNG QNILEVAEGN GPVSALDQAL RKALTRFYPQ IADFHLTDYK
     VRILDGGAGT SAKTRVLVES SNGDRRWTTV GVSGNILEAS YQAVVEGIEY GLRLLTCGLT
     NQEAISS