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CIMH_BACSU
ID   CIMH_BACSU              Reviewed;         450 AA.
AC   P94363;
DT   22-FEB-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1997, sequence version 1.
DT   03-AUG-2022, entry version 116.
DE   RecName: Full=Citrate/malate transporter;
DE   AltName: Full=Citrate/malate-proton symporter;
GN   Name=cimH; Synonyms=yxkJ; OrderedLocusNames=BSU38770;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168 / BGSC1A1;
RX   PubMed=8969509; DOI=10.1099/13500872-142-11-3113;
RA   Yoshida K., Shindo K., Sano H., Seki S., Fujimura M., Yanai N., Miwa Y.,
RA   Fujita Y.;
RT   "Sequencing of a 65 kb region of the Bacillus subtilis genome containing
RT   the lic and cel loci, and creation of a 177 kb contig covering the gnt-
RT   sacXY region.";
RL   Microbiology 142:3113-3123(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [3]
RP   CHARACTERIZATION.
RX   PubMed=11566984; DOI=10.1128/jb.183.20.5862-5869.2001;
RA   Krom B.P., Aardema R., Lolkema J.S.;
RT   "Bacillus subtilis YxkJ is a secondary transporter of the 2-
RT   hydroxycarboxylate transporter family that transports L-malate and
RT   citrate.";
RL   J. Bacteriol. 183:5862-5869(2001).
CC   -!- FUNCTION: Proton motive force-driven secondary transporter that
CC       catalyzes the uptake of both citrate and malate. Appears to be an
CC       electroneutral proton-solute symporter; the number of protons
CC       transported is equal to the valence of the transported anions. Is
CC       strictly stereoselective, translocating only the (S)-enantiomer of
CC       malate.
CC   -!- ACTIVITY REGULATION: The uptake activity is inhibited by divalent metal
CC       ions such as Ca(2+), Mg(2+) and Ni(2+).
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Multi-pass membrane
CC       protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the sodium:citrate (SCF) symporter family.
CC       {ECO:0000305}.
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DR   EMBL; D83026; BAA11726.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB15903.1; -; Genomic_DNA.
DR   PIR; C70081; C70081.
DR   RefSeq; NP_391756.1; NC_000964.3.
DR   RefSeq; WP_003227271.1; NZ_JNCM01000034.1.
DR   AlphaFoldDB; P94363; -.
DR   SMR; P94363; -.
DR   STRING; 224308.BSU38770; -.
DR   TCDB; 2.A.24.2.4; the 2-hydroxycarboxylate transporter (2-hct) family.
DR   PaxDb; P94363; -.
DR   PRIDE; P94363; -.
DR   EnsemblBacteria; CAB15903; CAB15903; BSU_38770.
DR   GeneID; 937407; -.
DR   KEGG; bsu:BSU38770; -.
DR   PATRIC; fig|224308.179.peg.4196; -.
DR   eggNOG; COG3493; Bacteria.
DR   InParanoid; P94363; -.
DR   OMA; IINACHS; -.
DR   PhylomeDB; P94363; -.
DR   BioCyc; BSUB:BSU38770-MON; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0015531; F:citrate:proton symporter activity; IDA:CACAO.
DR   GO; GO:0006101; P:citrate metabolic process; IEA:UniProtKB-KW.
DR   InterPro; IPR018025; 2-OHcarbox_trans_Prot/Firm.
DR   InterPro; IPR004679; 2-OHcarboxylate_transport.
DR   PANTHER; PTHR40033; PTHR40033; 1.
DR   Pfam; PF03390; 2HCT; 1.
DR   PIRSF; PIRSF005348; YxkH; 1.
DR   TIGRFAMs; TIGR00783; ccs; 1.
PE   1: Evidence at protein level;
KW   Cell membrane; Citrate utilization; Membrane; Reference proteome; Symport;
KW   Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..450
FT                   /note="Citrate/malate transporter"
FT                   /id="PRO_0000088761"
FT   TRANSMEM        32..52
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        62..82
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        88..108
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        120..140
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        153..173
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        219..239
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        275..295
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        302..322
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        333..353
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        368..388
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   REGION          249..268
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        249..264
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   450 AA;  48339 MW;  FC852E2155115607 CRC64;
     MGELQTHMQL QTDTIHEGVR KENWFAKAMN IKVGIIPLPV YALLFILITV FVMHHDVKSD
     ILTSIAVMAF FGFTFAQIGK SIPIVRSIGG PAILATFIPS AVVYYHLLPN DIVKSTTEFT
     ENSNFLYLFI AGIVVGSILG MKRETLVKAF MKIFIPLIVG SVTAAIVGLA VGTLLGLGFQ
     HTLLYIVIPI MAGGVGEGAI PLSIGYSDIM PISQGEAFAL VLPSIMLGSL CAIILAGLLN
     RIGKKKPEWT GNGKVDRSEE ESPALEESQS GQQMFNLSLF ASGGILAVSL YLVGMLAHDF
     FGFPAPVAML LLAVLIKLFR LVPASIENGA FGVSRFFSTA VTYPLLFAIG VSMTPWDKLV
     AAFNLSNIIT ILSVVVTMMA VGFFTGKWLN MYPIETAIIN ACHSGQGGTG DVAILSAAER
     LELMPFAQVS TRIGGAITVS LTLLLLHQFY
 
 
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