ID   ACG_MYCTU               Reviewed;         331 AA.
AC   P9WIZ9; L0TB56; O53476; Q7D7L2;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   03-AUG-2022, entry version 37.
DE   RecName: Full=Putative NAD(P)H nitroreductase acg;
DE            EC=1.-.-.-;
GN   Name=acg; OrderedLocusNames=Rv2032;
OS   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=9634230; DOI=10.1038/31159;
RA   Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA   Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA   Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA   Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA   Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA   Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA   Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA   Barrell B.G.;
RT   "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT   genome sequence.";
RL   Nature 393:537-544(1998).
RN   [2]
RP   INDUCTION BY HYPOXIA.
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=11416222; DOI=10.1073/pnas.121172498;
RA   Sherman D.R., Voskuil M., Schnappinger D., Liao R., Harrell M.I.,
RA   Schoolnik G.K.;
RT   "Regulation of the Mycobacterium tuberculosis hypoxic response gene
RT   encoding alpha -crystallin.";
RL   Proc. Natl. Acad. Sci. U.S.A. 98:7534-7539(2001).
RN   [3]
RP   DISCUSSION OF SEQUENCE.
RX   PubMed=11854240; DOI=10.1128/iai.70.3.1518-1529.2002;
RA   Purkayastha A., McCue L.A., McDonough K.A.;
RT   "Identification of a Mycobacterium tuberculosis putative classical
RT   nitroreductase gene whose expression is coregulated with that of the acr
RT   gene within macrophages, in standing versus shaking cultures, and under low
RT   oxygen conditions.";
RL   Infect. Immun. 70:1518-1529(2002).
RN   [4]
RP   INDUCTION BY NITRIC OXIDE (NO); BY HYPOXIA; IN MOUSE MODEL AND DORMANCY
RP   REGULON.
RX   PubMed=12953092; DOI=10.1084/jem.20030205;
RA   Voskuil M.I., Schnappinger D., Visconti K.C., Harrell M.I., Dolganov G.M.,
RA   Sherman D.R., Schoolnik G.K.;
RT   "Inhibition of respiration by nitric oxide induces a Mycobacterium
RT   tuberculosis dormancy program.";
RL   J. Exp. Med. 198:705-713(2003).
RN   [5]
RP   INDUCTION BY CARBON MONOXIDE (CO).
RC   STRAIN=ATCC 35801 / TMC 107 / Erdman;
RX   PubMed=18474359; DOI=10.1016/j.chom.2008.03.007;
RA   Shiloh M.U., Manzanillo P., Cox J.S.;
RT   "Mycobacterium tuberculosis senses host-derived carbon monoxide during
RT   macrophage infection.";
RL   Cell Host Microbe 3:323-330(2008).
RN   [6]
RP   INDUCTION BY CARBON MONOXIDE (CO), AND DORMANCY REGULON.
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=18400743; DOI=10.1074/jbc.m802274200;
RA   Kumar A., Deshane J.S., Crossman D.K., Bolisetty S., Yan B.S., Kramnik I.,
RA   Agarwal A., Steyn A.J.;
RT   "Heme oxygenase-1-derived carbon monoxide induces the Mycobacterium
RT   tuberculosis dormancy regulon.";
RL   J. Biol. Chem. 283:18032-18039(2008).
RN   [7]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=21969609; DOI=10.1074/mcp.m111.011627;
RA   Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K.,
RA   Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R.,
RA   Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M.,
RA   Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.;
RT   "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution
RT   mass spectrometry.";
RL   Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011).
CC   -!- COFACTOR:
CC       Name=FMN; Xref=ChEBI:CHEBI:58210; Evidence={ECO:0000305};
CC   -!- INDUCTION: A member of the dormancy regulon. Induced in response to
CC       reduced oxygen tension (hypoxia), low levels of nitric oxide (NO) and
CC       carbon monoxide (CO). It is hoped that this regulon will give insight
CC       into the latent, or dormant phase of infection. Induced in C57BL/6
CC       mouse lungs 3 weeks after low dose aerosol infection with H37Rv
CC       bacteria. {ECO:0000269|PubMed:11416222, ECO:0000269|PubMed:12953092,
CC       ECO:0000269|PubMed:18400743, ECO:0000269|PubMed:18474359}.
CC   -!- SIMILARITY: Belongs to the nitroreductase family. {ECO:0000305}.
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DR   EMBL; AL123456; CCP44805.1; -; Genomic_DNA.
DR   PIR; G70942; G70942.
DR   RefSeq; NP_216548.1; NC_000962.3.
DR   RefSeq; WP_003410193.1; NZ_NVQJ01000046.1.
DR   AlphaFoldDB; P9WIZ9; -.
DR   SMR; P9WIZ9; -.
DR   STRING; 83332.Rv2032; -.
DR   PaxDb; P9WIZ9; -.
DR   DNASU; 887582; -.
DR   GeneID; 887582; -.
DR   KEGG; mtu:Rv2032; -.
DR   TubercuList; Rv2032; -.
DR   eggNOG; COG0778; Bacteria.
DR   OMA; TITHMLE; -.
DR   PhylomeDB; P9WIZ9; -.
DR   Proteomes; UP000001584; Chromosome.
DR   GO; GO:0005829; C:cytosol; HDA:MTBBASE.
DR   GO; GO:0009274; C:peptidoglycan-based cell wall; HDA:MTBBASE.
DR   GO; GO:0005886; C:plasma membrane; HDA:MTBBASE.
DR   GO; GO:0016491; F:oxidoreductase activity; IBA:GO_Central.
DR   GO; GO:0052572; P:response to host immune response; IGI:MTBBASE.
DR   GO; GO:0001666; P:response to hypoxia; IGI:MTBBASE.
DR   Gene3D; 3.40.109.10; -; 2.
DR   InterPro; IPR000415; Nitroreductase-like.
DR   SUPFAM; SSF55469; SSF55469; 2.
PE   1: Evidence at protein level;
KW   Flavoprotein; FMN; NAD; NADP; Oxidoreductase; Reference proteome.
FT   CHAIN           1..331
FT                   /note="Putative NAD(P)H nitroreductase acg"
FT                   /id="PRO_0000392626"
FT   BINDING         28..32
FT                   /ligand="FMN"
FT                   /ligand_id="ChEBI:CHEBI:58210"
FT                   /evidence="ECO:0000255"
FT   BINDING         316
FT                   /ligand="FMN"
FT                   /ligand_id="ChEBI:CHEBI:58210"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   331 AA;  36559 MW;  8FF2A825CE90C6EB CRC64;
     MPDTMVTTDV IKSAVQLACR APSLHNSQPW RWIAEDHTVA LFLDKDRVLY ATDHSGREAL
     LGCGAVLDHF RVAMAAAGTT ANVERFPNPN DPLHLASIDF SPADFVTEGH RLRADAILLR
     RTDRLPFAEP PDWDLVESQL RTTVTADTVR IDVIADDMRP ELAAASKLTE SLRLYDSSYH
     AELFWWTGAF ETSEGIPHSS LVSAAESDRV TFGRDFPVVA NTDRRPEFGH DRSKVLVLST
     YDNERASLLR CGEMLSAVLL DATMAGLATC TLTHITELHA SRDLVAALIG QPATPQALVR
     VGLAPEMEEP PPATPRRPID EVFHVRAKDH R