ACH12_ARATH
ID ACH12_ARATH Reviewed; 366 AA.
AC Q9LTH7; Q2V2X0;
DT 03-MAY-2011, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 145.
DE RecName: Full=1-aminocyclopropane-1-carboxylate oxidase homolog 12;
DE EC=1.14.-.-;
GN OrderedLocusNames=At5g59540; ORFNames=F2O15.6;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RA Kaneko T., Katoh T., Asamizu E., Sato S., Nakamura Y., Kotani H.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. XI.";
RL Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RA Shinn P., Chen H., Kim C.J., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=Fe(2+); Xref=ChEBI:CHEBI:29033;
CC Evidence={ECO:0000255|PROSITE-ProRule:PRU00805};
CC Note=Binds 1 Fe(2+) ion per subunit. {ECO:0000255|PROSITE-
CC ProRule:PRU00805};
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9LTH7-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9LTH7-2; Sequence=VSP_041042, VSP_041043;
CC -!- SIMILARITY: Belongs to the iron/ascorbate-dependent oxidoreductase
CC family. {ECO:0000305}.
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DR EMBL; AB025604; BAA97488.1; -; Genomic_DNA.
DR EMBL; CP002688; AED97202.1; -; Genomic_DNA.
DR EMBL; CP002688; AED97203.1; -; Genomic_DNA.
DR EMBL; AF424616; AAL11609.1; -; mRNA.
DR EMBL; BT024895; ABD85166.1; -; mRNA.
DR EMBL; AK226221; BAE98386.1; -; mRNA.
DR RefSeq; NP_001032104.1; NM_001037027.1. [Q9LTH7-2]
DR RefSeq; NP_200762.1; NM_125346.3. [Q9LTH7-1]
DR AlphaFoldDB; Q9LTH7; -.
DR SMR; Q9LTH7; -.
DR iPTMnet; Q9LTH7; -.
DR PaxDb; Q9LTH7; -.
DR PRIDE; Q9LTH7; -.
DR ProteomicsDB; 243281; -. [Q9LTH7-1]
DR EnsemblPlants; AT5G59540.1; AT5G59540.1; AT5G59540. [Q9LTH7-1]
DR EnsemblPlants; AT5G59540.2; AT5G59540.2; AT5G59540. [Q9LTH7-2]
DR GeneID; 836073; -.
DR Gramene; AT5G59540.1; AT5G59540.1; AT5G59540. [Q9LTH7-1]
DR Gramene; AT5G59540.2; AT5G59540.2; AT5G59540. [Q9LTH7-2]
DR KEGG; ath:AT5G59540; -.
DR Araport; AT5G59540; -.
DR TAIR; locus:2148303; AT5G59540.
DR eggNOG; KOG0143; Eukaryota.
DR HOGENOM; CLU_010119_0_0_1; -.
DR InParanoid; Q9LTH7; -.
DR OMA; TAISHYQ; -.
DR OrthoDB; 755305at2759; -.
DR PhylomeDB; Q9LTH7; -.
DR BioCyc; ARA:AT5G59540-MON; -.
DR PRO; PR:Q9LTH7; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9LTH7; baseline and differential.
DR Genevisible; Q9LTH7; AT.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR Gene3D; 2.60.120.330; -; 1.
DR InterPro; IPR026992; DIOX_N.
DR InterPro; IPR044861; IPNS-like_FE2OG_OXY.
DR InterPro; IPR027443; IPNS-like_sf.
DR InterPro; IPR005123; Oxoglu/Fe-dep_dioxygenase.
DR Pfam; PF03171; 2OG-FeII_Oxy; 1.
DR Pfam; PF14226; DIOX_N; 1.
DR PROSITE; PS51471; FE2OG_OXY; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Iron; Metal-binding; Oxidoreductase;
KW Reference proteome.
FT CHAIN 1..366
FT /note="1-aminocyclopropane-1-carboxylate oxidase homolog
FT 12"
FT /id="PRO_0000408287"
FT DOMAIN 215..314
FT /note="Fe2OG dioxygenase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00805"
FT BINDING 239
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00805"
FT BINDING 241
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00805"
FT BINDING 295
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00805"
FT BINDING 305
FT /ligand="2-oxoglutarate"
FT /ligand_id="ChEBI:CHEBI:16810"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00805"
FT VAR_SEQ 284..285
FT /note="LI -> VK (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_041042"
FT VAR_SEQ 286..366
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_041043"
SQ SEQUENCE 366 AA; 41539 MW; 8C80B52FE06DD4A5 CRC64;
MMTKNSIEFD PYIERKAFDE TKQGVKGLVD AKITEVPRIF HHRQDILTNK KPSASVSDLE
IPIIDFASVH ADTASREAIV EKVKYAVENW GFFQVINHSI PLNVLEEIKD GVRRFHEEDP
EVKKSFFSRD AGNKKFVYNS NFDLYSSSPS VNWRDSFSCY IAPDPPAPEE IPETCRDAMF
EYSKHVLSFG GLLFELLSEA LGLKSQTLES MDCVKTLLMI CHYYPPCPQP DLTLGITKHS
DNSFLTLLLQ DNIGGLQILH QDSWVDVSPI HGALVVNIGD FLQLITNDKF VSVEHRVLAN
RQGPRISVAS FFSSSMRPNS RVYGPMKELV SEENPPKYRD ITIKEYSKIF FEKGLDGTSH
LSNIRI
