ACH1_DROME
ID ACH1_DROME Reviewed; 567 AA.
AC P09478; Q9VC74;
DT 01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT 09-MAY-2003, sequence version 2.
DT 03-AUG-2022, entry version 191.
DE RecName: Full=Acetylcholine receptor subunit alpha-like 1;
DE AltName: Full=Nicotinic acetylcholine receptor alpha 1;
DE Flags: Precursor;
GN Name=nAChRalpha1; Synonyms=Acr96Aa, AcrB, als, nAcRalpha-96Aa;
GN ORFNames=CG5610;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RC STRAIN=Oregon-R;
RX PubMed=2840281; DOI=10.1002/j.1460-2075.1988.tb02854.x;
RA Bossy B., Ballivet M., Spierer P.;
RT "Conservation of neural nicotinic acetylcholine receptors from Drosophila
RT to vertebrate central nervous systems.";
RL EMBO J. 7:611-618(1988).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
CC -!- FUNCTION: After binding acetylcholine, the AChR responds by an
CC extensive change in conformation that affects all subunits and leads to
CC opening of an ion-conducting channel across the plasma membrane.
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Postsynaptic cell membrane; Multi-pass membrane
CC protein. Cell membrane {ECO:0000250}; Multi-pass membrane protein
CC {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: CNS in embryos. {ECO:0000269|PubMed:2840281}.
CC -!- DEVELOPMENTAL STAGE: Late embryonic, late pupal and second instar
CC larvae stages. {ECO:0000269|PubMed:2840281}.
CC -!- SIMILARITY: Belongs to the ligand-gated ion channel (TC 1.A.9) family.
CC Acetylcholine receptor (TC 1.A.9.1) subfamily. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; X07194; CAA30172.1; -; mRNA.
DR EMBL; AE014297; AAF56301.2; -; Genomic_DNA.
DR PIR; S00381; ACFFA1.
DR RefSeq; NP_001262917.1; NM_001275988.2.
DR RefSeq; NP_524481.2; NM_079757.4.
DR AlphaFoldDB; P09478; -.
DR SMR; P09478; -.
DR BioGRID; 67844; 1.
DR STRING; 7227.FBpp0084003; -.
DR ChEMBL; CHEMBL3350223; -.
DR GlyGen; P09478; 2 sites.
DR PaxDb; P09478; -.
DR PRIDE; P09478; -.
DR EnsemblMetazoa; FBtr0084619; FBpp0084003; FBgn0000036.
DR EnsemblMetazoa; FBtr0334916; FBpp0306936; FBgn0000036.
DR GeneID; 42918; -.
DR KEGG; dme:Dmel_CG5610; -.
DR CTD; 42918; -.
DR FlyBase; FBgn0000036; nAChRalpha1.
DR VEuPathDB; VectorBase:FBgn0000036; -.
DR eggNOG; KOG3645; Eukaryota.
DR GeneTree; ENSGT00940000171907; -.
DR HOGENOM; CLU_018074_1_0_1; -.
DR InParanoid; P09478; -.
DR OMA; QHAKNKD; -.
DR OrthoDB; 381858at2759; -.
DR PhylomeDB; P09478; -.
DR Reactome; R-DME-629594; Highly calcium permeable postsynaptic nicotinic acetylcholine receptors.
DR BioGRID-ORCS; 42918; 0 hits in 3 CRISPR screens.
DR GenomeRNAi; 42918; -.
DR PRO; PR:P09478; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0000036; Expressed in central nervous system and 6 other tissues.
DR ExpressionAtlas; P09478; baseline and differential.
DR Genevisible; P09478; DM.
DR GO; GO:0005892; C:acetylcholine-gated channel complex; IDA:FlyBase.
DR GO; GO:0070161; C:anchoring junction; IEA:UniProtKB-KW.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0043005; C:neuron projection; IBA:GO_Central.
DR GO; GO:0045211; C:postsynaptic membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0045202; C:synapse; IBA:GO_Central.
DR GO; GO:0022848; F:acetylcholine-gated cation-selective channel activity; IEA:InterPro.
DR GO; GO:0005231; F:excitatory extracellular ligand-gated ion channel activity; IBA:GO_Central.
DR GO; GO:0030594; F:neurotransmitter receptor activity; IBA:GO_Central.
DR GO; GO:0004888; F:transmembrane signaling receptor activity; IEA:InterPro.
DR GO; GO:1904315; F:transmitter-gated ion channel activity involved in regulation of postsynaptic membrane potential; IBA:GO_Central.
DR GO; GO:0098655; P:cation transmembrane transport; IDA:FlyBase.
DR GO; GO:0007268; P:chemical synaptic transmission; IBA:GO_Central.
DR GO; GO:0034220; P:ion transmembrane transport; IBA:GO_Central.
DR GO; GO:0050877; P:nervous system process; IBA:GO_Central.
DR GO; GO:0042391; P:regulation of membrane potential; IBA:GO_Central.
DR GO; GO:0017085; P:response to insecticide; IMP:FlyBase.
DR GO; GO:0007165; P:signal transduction; IBA:GO_Central.
DR GO; GO:0007271; P:synaptic transmission, cholinergic; IEP:FlyBase.
DR Gene3D; 1.20.58.390; -; 2.
DR Gene3D; 2.70.170.10; -; 1.
DR InterPro; IPR006202; Neur_chan_lig-bd.
DR InterPro; IPR036734; Neur_chan_lig-bd_sf.
DR InterPro; IPR006201; Neur_channel.
DR InterPro; IPR036719; Neuro-gated_channel_TM_sf.
DR InterPro; IPR038050; Neuro_actylchol_rec.
DR InterPro; IPR006029; Neurotrans-gated_channel_TM.
DR InterPro; IPR018000; Neurotransmitter_ion_chnl_CS.
DR InterPro; IPR002394; Nicotinic_acetylcholine_rcpt.
DR PANTHER; PTHR18945; PTHR18945; 2.
DR Pfam; PF02931; Neur_chan_LBD; 1.
DR Pfam; PF02932; Neur_chan_memb; 1.
DR PRINTS; PR00254; NICOTINICR.
DR PRINTS; PR00252; NRIONCHANNEL.
DR SUPFAM; SSF63712; SSF63712; 1.
DR SUPFAM; SSF90112; SSF90112; 1.
DR TIGRFAMs; TIGR00860; LIC; 1.
DR PROSITE; PS00236; NEUROTR_ION_CHANNEL; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Disulfide bond; Glycoprotein; Ion channel; Ion transport;
KW Ligand-gated ion channel; Membrane; Postsynaptic cell membrane; Receptor;
KW Reference proteome; Signal; Synapse; Transmembrane; Transmembrane helix;
KW Transport.
FT SIGNAL 1..21
FT CHAIN 22..567
FT /note="Acetylcholine receptor subunit alpha-like 1"
FT /id="PRO_0000000298"
FT TOPO_DOM 22..240
FT /note="Extracellular"
FT TRANSMEM 241..264
FT /note="Helical"
FT TRANSMEM 272..290
FT /note="Helical"
FT TRANSMEM 306..325
FT /note="Helical"
FT TOPO_DOM 326..513
FT /note="Cytoplasmic"
FT TRANSMEM 514..532
FT /note="Helical"
FT CARBOHYD 45
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 233
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 149..163
FT /evidence="ECO:0000250"
FT DISULFID 222..223
FT /note="Associated with receptor activation"
FT /evidence="ECO:0000250"
FT VARIANT 538
FT /note="Y -> H"
FT CONFLICT 108
FT /note="L -> H (in Ref. 1; CAA30172)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 567 AA; 64019 MW; 08E1F721FB2A92AC CRC64;
MGSVLFAAVF IALHFATGGL ANPDAKRLYD DLLSNYNRLI RPVGNNSDRL TVKMGLRLSQ
LIDVNLKNQI MTTNVWVEQE WNDYKLKWNP DDYGGVDTLH VPSEHIWLPD IVLYNNADGN
YEVTIMTKAI LHHTGKVVWK PPAIYKSFCE IDVEYFPFDE QTCFMKFGSW TYDGYMVDLR
HLKQTADSDN IEVGIDLQDY YISVEWDIMR VPAVRNEKFY SCCEEPYLDI VFNLTLRRKT
LFYTVNLIIP CVGISFLSVL VFYLPSDSGE KISLCISILL SLTVFFLLLA EIIPPTSLTV
PLLGKYLLFT MMLVTLSVVV TIAVLNVNFR SPVTHRMAPW VQRLFIQILP KLLCIERPKK
EEPEEDQPPE VLTDVYHLPP DVDKFVNYDS KRFSGDYGIP ALPASHRFDL AAAGGISAHC
FAEPPLPSSL PLPGADDDLF SPSGLNGDIS PGCCPAAAAA AAADLSPTFE KPYAREMEKT
IEGSRFIAQH VKNKDKFESV EEDWKYVAMV LDRMFLWIFA IACVVGTALI ILQAPSLYDQ
SQPIDILYSK IAKKKFELLK MGSENTL
