CINES_PICGL
ID CINES_PICGL Reviewed; 612 AA.
AC F2XFA2;
DT 23-FEB-2022, integrated into UniProtKB/Swiss-Prot.
DT 31-MAY-2011, sequence version 1.
DT 03-AUG-2022, entry version 39.
DE RecName: Full=1,8-cineole synthase, chloroplastic {ECO:0000303|PubMed:21385377, ECO:0000303|PubMed:26017574};
DE EC=4.2.3.108 {ECO:0000269|PubMed:21385377};
DE AltName: Full=Terpene synthase TPS-Cin {ECO:0000303|PubMed:21385377, ECO:0000303|PubMed:26017574};
DE Short=PgTPS-Cin {ECO:0000303|PubMed:21385377, ECO:0000303|PubMed:26017574};
DE Flags: Precursor;
GN Name=TPS-Cin {ECO:0000303|PubMed:21385377, ECO:0000303|PubMed:26017574};
OS Picea glauca (White spruce) (Pinus glauca).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Pinopsida; Pinidae; Conifers I; Pinales; Pinaceae; Picea.
OX NCBI_TaxID=3330;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], CATALYTIC ACTIVITY, FUNCTION, PATHWAY, AND GENE
RP FAMILY.
RC STRAIN=cv. PG29;
RX PubMed=21385377; DOI=10.1186/1471-2229-11-43;
RA Keeling C.I., Weisshaar S., Ralph S.G., Jancsik S., Hamberger B.,
RA Dullat H.K., Bohlmann J.;
RT "Transcriptome mining, functional characterization, and phylogeny of a
RT large terpene synthase gene family in spruce (Picea spp.).";
RL BMC Plant Biol. 11:43-43(2011).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=26017574; DOI=10.1111/tpj.12886;
RA Warren R.L., Keeling C.I., Yuen M.M.S., Raymond A., Taylor G.A.,
RA Vandervalk B.P., Mohamadi H., Paulino D., Chiu R., Jackman S.D.,
RA Robertson G., Yang C., Hoffmann M., Weigel D., Nelson D.R., Ritland C.,
RA Isabel N., Jaquish B., Yanchuk A., Bousquet J., Jones S.J.M., MacKay J.,
RA Birol I., Bohlmann J.;
RT "Improved white spruce (Picea glauca) genome assemblies and annotation of
RT large gene families of conifer terpenoid and phenolic defense metabolism.";
RL Plant J. 83:189-212(2015).
CC -!- FUNCTION: Terpene synthase (TPS) involved in the biosynthesis of
CC monoterpene natural products included in conifer oleoresin secretions
CC and volatile emissions; these compounds contribute to biotic and
CC abiotic stress defense against herbivores and pathogens
CC (PubMed:21385377). Catalyzes the conversion of (2E)-geranyl diphosphate
CC (GPP) to 1,8-cineole (PubMed:21385377). {ECO:0000269|PubMed:21385377}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(2E)-geranyl diphosphate + H2O = 1,8-cineole + diphosphate;
CC Xref=Rhea:RHEA:32543, ChEBI:CHEBI:15377, ChEBI:CHEBI:27961,
CC ChEBI:CHEBI:33019, ChEBI:CHEBI:58057; EC=4.2.3.108;
CC Evidence={ECO:0000269|PubMed:21385377};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000250|UniProtKB:A0A1C9J6A7};
CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC Evidence={ECO:0000250|UniProtKB:A0A1C9J6A7};
CC Note=Binds 3 Mg(2+) or Mn(2+) ions per subunit.
CC {ECO:0000250|UniProtKB:A0A1C9J6A7};
CC -!- PATHWAY: Terpene metabolism; oleoresin biosynthesis.
CC {ECO:0000269|PubMed:21385377}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000255}.
CC -!- DOMAIN: The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important for
CC the catalytic activity, presumably through binding to Mg(2+).
CC {ECO:0000250|UniProtKB:A0A1C9J6A7}.
CC -!- SIMILARITY: Belongs to the terpene synthase family. Tpsd subfamily.
CC {ECO:0000305}.
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DR EMBL; HQ426160; ADZ45498.1; -; mRNA.
DR EMBL; GCZO01133025; JAI17753.1; -; Transcribed_RNA.
DR UniPathway; UPA00924; -.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0102313; F:1,8-cineole synthase activity; IDA:UniProtKB.
DR GO; GO:0016829; F:lyase activity; IDA:UniProtKB.
DR GO; GO:0000287; F:magnesium ion binding; IEA:InterPro.
DR GO; GO:0010333; F:terpene synthase activity; IEA:InterPro.
DR GO; GO:0016102; P:diterpenoid biosynthetic process; IEA:InterPro.
DR GO; GO:0010597; P:green leaf volatile biosynthetic process; IDA:UniProtKB.
DR GO; GO:0016099; P:monoterpenoid biosynthetic process; IDA:UniProtKB.
DR CDD; cd00684; Terpene_cyclase_plant_C1; 1.
DR Gene3D; 1.10.600.10; -; 1.
DR Gene3D; 1.50.10.130; -; 1.
DR InterPro; IPR008949; Isoprenoid_synthase_dom_sf.
DR InterPro; IPR034741; Terpene_cyclase-like_1_C.
DR InterPro; IPR044814; Terpene_cyclase_plant_C1.
DR InterPro; IPR001906; Terpene_synth_N.
DR InterPro; IPR036965; Terpene_synth_N_sf.
DR InterPro; IPR005630; Terpene_synthase_metal-bd.
DR InterPro; IPR008930; Terpenoid_cyclase/PrenylTrfase.
DR Pfam; PF01397; Terpene_synth; 1.
DR Pfam; PF03936; Terpene_synth_C; 1.
DR SFLD; SFLDG01019; Terpene_Cyclase_Like_1_C_Termi; 1.
DR SUPFAM; SSF48239; SSF48239; 1.
DR SUPFAM; SSF48576; SSF48576; 1.
PE 1: Evidence at protein level;
KW Chloroplast; Lyase; Magnesium; Metal-binding; Plastid; Transit peptide.
FT TRANSIT 1..52
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 53..612
FT /note="1,8-cineole synthase, chloroplastic"
FT /id="PRO_0000454402"
FT MOTIF 363..367
FT /note="DDXXD motif"
FT /evidence="ECO:0000250|UniProtKB:A0A1C9J6A7"
FT BINDING 363
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="1"
FT /evidence="ECO:0000250|UniProtKB:Q40577"
FT BINDING 363
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="2"
FT /evidence="ECO:0000250|UniProtKB:Q40577"
FT BINDING 367
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="1"
FT /evidence="ECO:0000250|UniProtKB:Q40577"
FT BINDING 367
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="2"
FT /evidence="ECO:0000250|UniProtKB:Q40577"
FT BINDING 515
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="3"
FT /evidence="ECO:0000250|UniProtKB:Q40577"
SQ SEQUENCE 612 AA; 70237 MW; 3C1C3FA649D740BA CRC64;
MALVCGAPLA SRSCLNKSLI SSTHELKPLR RTILPTLRWK SATPSINMCL TTSNSVDAVQ
RRIANHHSNL WDDDFIQSLS TPYEAPSYRE RAERLIGEVK EMFESMGPNN DLLQRLSMVE
SVERLGIDRH FKNEIKSALD YVYSHWNEKG IGCGRDSVVS DLNSTALALR TLRLHGYPVS
SDVLEHFKDQ KGRFACSSIK TEGEIRSLLN LFRASLVAFP NEKVMEEAEI FSTTYLKEAV
QKIPVSSLSR QIEYNMEYGW HTNLPRLEAR NYMGDMIHVM PYMNAEKLLE LAKLEFNIFH
SLQERELKHL SRWWKDSGFS QLTFVRHRHV EYYTLASCID IDPKHSAFRL GFAKMCHLIT
VLDDIYDTFG TMDELKLFTA AIKRWDPSAT EWLPEYMKGV YMVVYETVNE MAGEAKKSQG
RDTINYARQA WEAYIDSYMK EAEWISSGCL PTFEEYYENG KVSFGYQISV LQPILTLDVP
LPHHILQEII FPSRFNGLAS GILRLKGDTR CYQADRARGE EASCISCYMN DNPGATEEDA
LNHIHAMVNE LMKEFNWELL KPDNNVPVSS KKHAFDITRA VHHGYKYRDG YSVANNEIKN
LVITTVLEPV PL
