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CINES_PICXS
ID   CINES_PICXS             Reviewed;         612 AA.
AC   F2XF98;
DT   23-FEB-2022, integrated into UniProtKB/Swiss-Prot.
DT   31-MAY-2011, sequence version 1.
DT   03-AUG-2022, entry version 38.
DE   RecName: Full=1,8-cineole synthase, chloroplastic {ECO:0000303|PubMed:21385377};
DE            EC=4.2.3.108 {ECO:0000269|PubMed:21385377};
DE   AltName: Full=Terpene synthase TPS-Cin {ECO:0000303|PubMed:21385377};
DE            Short=PgxeTPS-Cin {ECO:0000303|PubMed:21385377};
DE   Flags: Precursor;
GN   Name=TPS-Cin {ECO:0000303|PubMed:21385377};
OS   Picea engelmannii x Picea glauca (Hybrid white spruce).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Pinopsida; Pinidae; Conifers I; Pinales; Pinaceae; Picea.
OX   NCBI_TaxID=373101;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], CATALYTIC ACTIVITY, FUNCTION, PATHWAY, AND GENE
RP   FAMILY.
RC   STRAIN=cv. Fa1-1028;
RX   PubMed=21385377; DOI=10.1186/1471-2229-11-43;
RA   Keeling C.I., Weisshaar S., Ralph S.G., Jancsik S., Hamberger B.,
RA   Dullat H.K., Bohlmann J.;
RT   "Transcriptome mining, functional characterization, and phylogeny of a
RT   large terpene synthase gene family in spruce (Picea spp.).";
RL   BMC Plant Biol. 11:43-43(2011).
CC   -!- FUNCTION: Terpene synthase (TPS) involved in the biosynthesis of
CC       monoterpene natural products included in conifer oleoresin secretions
CC       and volatile emissions; these compounds contribute to biotic and
CC       abiotic stress defense against herbivores and pathogens
CC       (PubMed:21385377). Catalyzes the conversion of (2E)-geranyl diphosphate
CC       (GPP) to 1,8-cineole (PubMed:21385377). {ECO:0000269|PubMed:21385377}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(2E)-geranyl diphosphate + H2O = 1,8-cineole + diphosphate;
CC         Xref=Rhea:RHEA:32543, ChEBI:CHEBI:15377, ChEBI:CHEBI:27961,
CC         ChEBI:CHEBI:33019, ChEBI:CHEBI:58057; EC=4.2.3.108;
CC         Evidence={ECO:0000269|PubMed:21385377};
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000250|UniProtKB:A0A1C9J6A7};
CC       Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC         Evidence={ECO:0000250|UniProtKB:A0A1C9J6A7};
CC       Note=Binds 3 Mg(2+) or Mn(2+) ions per subunit.
CC       {ECO:0000250|UniProtKB:A0A1C9J6A7};
CC   -!- PATHWAY: Terpene metabolism; oleoresin biosynthesis.
CC       {ECO:0000269|PubMed:21385377}.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000255}.
CC   -!- DOMAIN: The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important for
CC       the catalytic activity, presumably through binding to Mg(2+).
CC       {ECO:0000250|UniProtKB:A0A1C9J6A7}.
CC   -!- SIMILARITY: Belongs to the terpene synthase family. Tpsd subfamily.
CC       {ECO:0000305}.
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DR   EMBL; HQ426156; ADZ45497.1; -; mRNA.
DR   UniPathway; UPA00924; -.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0102313; F:1,8-cineole synthase activity; IDA:UniProtKB.
DR   GO; GO:0016829; F:lyase activity; IDA:UniProtKB.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:InterPro.
DR   GO; GO:0010333; F:terpene synthase activity; IEA:InterPro.
DR   GO; GO:0016102; P:diterpenoid biosynthetic process; IEA:InterPro.
DR   GO; GO:0010597; P:green leaf volatile biosynthetic process; IDA:UniProtKB.
DR   GO; GO:0016099; P:monoterpenoid biosynthetic process; IDA:UniProtKB.
DR   CDD; cd00684; Terpene_cyclase_plant_C1; 1.
DR   Gene3D; 1.10.600.10; -; 1.
DR   Gene3D; 1.50.10.130; -; 1.
DR   InterPro; IPR008949; Isoprenoid_synthase_dom_sf.
DR   InterPro; IPR034741; Terpene_cyclase-like_1_C.
DR   InterPro; IPR044814; Terpene_cyclase_plant_C1.
DR   InterPro; IPR001906; Terpene_synth_N.
DR   InterPro; IPR036965; Terpene_synth_N_sf.
DR   InterPro; IPR005630; Terpene_synthase_metal-bd.
DR   InterPro; IPR008930; Terpenoid_cyclase/PrenylTrfase.
DR   Pfam; PF01397; Terpene_synth; 1.
DR   Pfam; PF03936; Terpene_synth_C; 1.
DR   SFLD; SFLDG01019; Terpene_Cyclase_Like_1_C_Termi; 1.
DR   SUPFAM; SSF48239; SSF48239; 1.
DR   SUPFAM; SSF48576; SSF48576; 1.
PE   1: Evidence at protein level;
KW   Chloroplast; Lyase; Magnesium; Metal-binding; Plastid; Transit peptide.
FT   TRANSIT         1..52
FT                   /note="Chloroplast"
FT                   /evidence="ECO:0000255"
FT   CHAIN           53..612
FT                   /note="1,8-cineole synthase, chloroplastic"
FT                   /id="PRO_0000454403"
FT   MOTIF           363..367
FT                   /note="DDXXD motif"
FT                   /evidence="ECO:0000250|UniProtKB:A0A1C9J6A7"
FT   BINDING         363
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250|UniProtKB:Q40577"
FT   BINDING         363
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250|UniProtKB:Q40577"
FT   BINDING         367
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250|UniProtKB:Q40577"
FT   BINDING         367
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250|UniProtKB:Q40577"
FT   BINDING         515
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="3"
FT                   /evidence="ECO:0000250|UniProtKB:Q40577"
SQ   SEQUENCE   612 AA;  70254 MW;  C8F0E50CE59353CE CRC64;
     MALVSGAPLA SRSCLNKSLI SSTHELKPLR RTILPTLRWK SATPSINMCL TTSNSVDAVQ
     RRIANHHSNL WDDDFIQSLS TPYEAPSYRE RAERLIGEVK EMFESMGPNN DLLQRLSMVE
     SVERLGIDRH FKNEIKSALD YVYSHWNEKG IGCGRDSVVS DLNSTALALR TLRLHGYPVS
     SDVLEHFKDQ KGRFACSSIK TEGEIRSLLN LFRASLVAFP NEKVMEEAEI FSTTYLKEAV
     QKIPVSSLSR QIEYNMEYGW HTNLPRLEAR NYMGDMIHEM SYMNAEKLLE LAKLEFNIFH
     SLQERELKHL SRWWKDSGFS QLNFVRHRHV EYYTLASCID IDPKHSAFRL GFAKMCHLIT
     VLDDIYDTFG TMDELKLFTA AIKRWDPSAT EWLPEYMKGV YMVVYETVNE MAGEAKKSQG
     RDTINYARQA WEAYIDSYMK EAEWISSGCL PTFEEYYENG KVSFGYQISV LQPILTLDVP
     LPHHILQEII FPSRFNGLAS GILRLKGDTR CYQADRARGE EASCISCYMN DNPGATEEDA
     LNHIHAMVNE LMKEFNWELL KPDNNVPVSS KKHAFDITRA VHHGYKYRDG YSVANNEIKN
     LVITTVLEPV PL
 
 
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