ID   CINV1_ORYSJ             Reviewed;         562 AA.
AC   Q69T31; A0A0P0VKC8;
DT   07-JAN-2015, integrated into UniProtKB/Swiss-Prot.
DT   25-OCT-2004, sequence version 1.
DT   03-AUG-2022, entry version 113.
DE   RecName: Full=Cytosolic invertase 1 {ECO:0000303|PubMed:18317796};
DE            Short=OsCYT-INV1 {ECO:0000303|PubMed:18317796};
DE            EC=3.2.1.26 {ECO:0000269|PubMed:18317796};
GN   Name=CINV1 {ECO:0000305};
GN   OrderedLocusNames=Os02g0550600 {ECO:0000312|EMBL:BAF09009.1},
GN   LOC_Os02g34560 {ECO:0000305};
GN   ORFNames=OsJ_07095 {ECO:0000312|EMBL:EAZ23402.1},
GN   P0451A10.27 {ECO:0000312|EMBL:BAD33266.1};
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=18089549; DOI=10.1093/nar/gkm978;
RG   The rice annotation project (RAP);
RT   "The rice annotation project database (RAP-DB): 2008 update.";
RL   Nucleic Acids Res. 36:D1028-D1033(2008).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA   Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA   Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA   Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using next
RT   generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA   Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA   Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA   Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA   Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA   Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA   Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA   Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA   Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA   Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA   Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA   Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA   Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA   Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA   McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT   "The genomes of Oryza sativa: a history of duplications.";
RL   PLoS Biol. 3:266-281(2005).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=12869764; DOI=10.1126/science.1081288;
RG   The rice full-length cDNA consortium;
RT   "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT   japonica rice.";
RL   Science 301:376-379(2003).
RN   [6]
RP   FUNCTION, CATALYTIC ACTIVITY, TISSUE SPECIFICITY, AND MUTAGENESIS OF
RP   GLY-205.
RX   PubMed=18317796; DOI=10.1007/s00425-008-0718-0;
RA   Jia L., Zhang B., Mao C., Li J., Wu Y., Wu P., Wu Z.;
RT   "OsCYT-INV1 for alkaline/neutral invertase is involved in root cell
RT   development and reproductivity in rice (Oryza sativa L.).";
RL   Planta 228:51-59(2008).
CC   -!- FUNCTION: Cytosolic invertase that cleaves sucrose into glucose and
CC       fructose and is involved in the regulation of primary root elongation,
CC       lateral root formation, floral transition and pollen development.
CC       {ECO:0000269|PubMed:18317796}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Hydrolysis of terminal non-reducing beta-D-fructofuranoside
CC         residues in beta-D-fructofuranosides.; EC=3.2.1.26;
CC         Evidence={ECO:0000269|PubMed:18317796};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytosol
CC       {ECO:0000250|UniProtKB:Q9LQF2}.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 100 family.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AP004775; BAD33266.1; -; Genomic_DNA.
DR   EMBL; AP008208; BAF09009.1; -; Genomic_DNA.
DR   EMBL; AP014958; BAS79160.1; -; Genomic_DNA.
DR   EMBL; CM000139; EAZ23402.1; -; Genomic_DNA.
DR   EMBL; AK102741; BAG95698.1; -; mRNA.
DR   RefSeq; XP_015625957.1; XM_015770471.1.
DR   AlphaFoldDB; Q69T31; -.
DR   SMR; Q69T31; -.
DR   STRING; 4530.OS02T0550600-01; -.
DR   CAZy; GH100; Glycoside Hydrolase Family 100.
DR   iPTMnet; Q69T31; -.
DR   PaxDb; Q69T31; -.
DR   PRIDE; Q69T31; -.
DR   EnsemblPlants; Os02t0550600-01; Os02t0550600-01; Os02g0550600.
DR   GeneID; 4329626; -.
DR   Gramene; Os02t0550600-01; Os02t0550600-01; Os02g0550600.
DR   KEGG; osa:4329626; -.
DR   eggNOG; ENOG502QPS0; Eukaryota.
DR   HOGENOM; CLU_020846_1_1_1; -.
DR   InParanoid; Q69T31; -.
DR   OMA; GRPDFRF; -.
DR   OrthoDB; 344496at2759; -.
DR   PlantReactome; R-OSA-9030654; Primary root development.
DR   Proteomes; UP000000763; Chromosome 2.
DR   Proteomes; UP000007752; Chromosome 2.
DR   Proteomes; UP000059680; Chromosome 2.
DR   Genevisible; Q69T31; OS.
DR   GO; GO:0005829; C:cytosol; IEA:UniProtKB-SubCell.
DR   GO; GO:0033926; F:glycopeptide alpha-N-acetylgalactosaminidase activity; IEA:InterPro.
DR   GO; GO:0004575; F:sucrose alpha-glucosidase activity; IMP:UniProtKB.
DR   GO; GO:0010311; P:lateral root formation; IMP:UniProtKB.
DR   GO; GO:0009555; P:pollen development; IMP:UniProtKB.
DR   GO; GO:0080022; P:primary root development; IMP:UniProtKB.
DR   GO; GO:0048510; P:regulation of timing of transition from vegetative to reproductive phase; IMP:UniProtKB.
DR   GO; GO:0005987; P:sucrose catabolic process; IMP:UniProtKB.
DR   Gene3D; 1.50.10.10; -; 1.
DR   InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR   InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR   InterPro; IPR024746; Glyco_hydro_100.
DR   PANTHER; PTHR31916; PTHR31916; 1.
DR   Pfam; PF12899; Glyco_hydro_100; 1.
DR   SUPFAM; SSF48208; SSF48208; 1.
PE   1: Evidence at protein level;
KW   Carbohydrate metabolism; Cytoplasm; Glycosidase; Hydrolase;
KW   Reference proteome.
FT   CHAIN           1..562
FT                   /note="Cytosolic invertase 1"
FT                   /id="PRO_0000431504"
FT   MUTAGEN         205
FT                   /note="G->R: Short root, delayed flowering, and partial
FT                   sterility.; increased sucrose accumulation and reduction of
FT                   hexose levels."
FT                   /evidence="ECO:0000269|PubMed:18317796"
SQ   SEQUENCE   562 AA;  63451 MW;  6FA33866E0FAC8B6 CRC64;
     MELAVGAGGM RRSASHTSLS ESDDFDLSRL LNKPRINVER QRSFDDRSLS DVSYSGGGHG
     GTRGGFDGMY SPGGGLRSLV GTPASSALHS FEPHPIVGDA WEALRRSLVF FRGQPLGTIA
     AFDHASEEVL NYDQVFVRDF VPSALAFLMN GEPEIVRHFL LKTLLLQGWE KKVDRFKLGE
     GAMPASFKVL HDSKKGVDTL HADFGESAIG RVAPVDSGFW WIILLRAYTK STGDLTLAET
     PECQKGMRLI LSLCLSEGFD TFPTLLCADG CCMIDRRMGV YGYPIEIQAL FFMALRCALQ
     LLKHDNEGKE FVERIATRLH ALSYHMRSYY WLDFQQLNDI YRYKTEEYSH TAVNKFNVIP
     DSIPDWLFDF MPCQGGFFIG NVSPARMDFR WFALGNMIAI LSSLATPEQS TAIMDLIEER
     WEELIGEMPL KICYPAIENH EWRIVTGCDP KNTRWSYHNG GSWPVLLWLL TAACIKTGRP
     QIARRAIDLA ERRLLKDGWP EYYDGKLGRY VGKQARKFQT WSIAGYLVAK MMLEDPSHLG
     MISLEEDKAM KPVLKRSASW TN