CIN_NICSU
ID CIN_NICSU Reviewed; 610 AA.
AC A5Y5L5;
DT 23-FEB-2022, integrated into UniProtKB/Swiss-Prot.
DT 10-JUL-2007, sequence version 1.
DT 03-AUG-2022, entry version 56.
DE RecName: Full=1,8-cineol synthase, chloroplastic {ECO:0000303|PubMed:17611797};
DE EC=4.2.3.108 {ECO:0000269|PubMed:17611797};
DE AltName: Full=(E)-beta-ocimene synthase {ECO:0000303|PubMed:17611797};
DE EC=4.2.3.106 {ECO:0000269|PubMed:17611797};
DE AltName: Full=Alpha-pinene synthase {ECO:0000303|PubMed:17611797};
DE EC=4.2.3.- {ECO:0000269|PubMed:17611797};
DE AltName: Full=Alpha-terpineol synthase {ECO:0000303|PubMed:17611797};
DE EC=4.2.3.111 {ECO:0000269|PubMed:17611797};
DE AltName: Full=Beta-myrcene synthase {ECO:0000303|PubMed:17611797};
DE EC=4.2.3.15 {ECO:0000269|PubMed:17611797};
DE AltName: Full=Limonene synthase {ECO:0000303|PubMed:17611797};
DE EC=4.2.3.- {ECO:0000269|PubMed:17611797};
DE AltName: Full=Sabinene synthase {ECO:0000303|PubMed:17611797};
DE EC=4.2.3.- {ECO:0000269|PubMed:17611797};
DE Flags: Precursor;
GN Name=CIN {ECO:0000303|PubMed:17611797};
OS Nicotiana suaveolens (Australian tobacco).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae;
OC Nicotiana.
OX NCBI_TaxID=200320;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, PATHWAY, TISSUE
RP SPECIFICITY, DEVELOPMENTAL STAGE, INDUCTION, BIOPHYSICOCHEMICAL PROPERTIES,
RP AND COFACTOR.
RC TISSUE=Flower;
RX PubMed=17611797; DOI=10.1007/s11103-007-9202-7;
RA Roeder S., Hartmann A.-M., Effmert U., Piechulla B.;
RT "Regulation of simultaneous synthesis of floral scent terpenoids by the
RT 1,8-cineole synthase of Nicotiana suaveolens.";
RL Plant Mol. Biol. 65:107-124(2007).
CC -!- FUNCTION: Monoterpene synthase involved in the biosynthesis of
CC monoterpene natural products of the 'cineole cassette', volatile
CC compounds present in floral scent (PubMed:17611797). Catalyzes the
CC conversion of (2E)-geranyl diphosphate (GPP) into 1,8-cineole and, as
CC minor products, limonene, sabinene, (E)-beta-ocimene, beta-myrcene,
CC alpha-pinene and alpha-terpineol (PubMed:17611797).
CC {ECO:0000269|PubMed:17611797}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(2E)-geranyl diphosphate + H2O = 1,8-cineole + diphosphate;
CC Xref=Rhea:RHEA:32543, ChEBI:CHEBI:15377, ChEBI:CHEBI:27961,
CC ChEBI:CHEBI:33019, ChEBI:CHEBI:58057; EC=4.2.3.108;
CC Evidence={ECO:0000269|PubMed:17611797};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:32544;
CC Evidence={ECO:0000269|PubMed:17611797};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(2E)-geranyl diphosphate = diphosphate + limonene;
CC Xref=Rhea:RHEA:68640, ChEBI:CHEBI:15384, ChEBI:CHEBI:33019,
CC ChEBI:CHEBI:58057; Evidence={ECO:0000269|PubMed:17611797};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:68641;
CC Evidence={ECO:0000269|PubMed:17611797};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(2E)-geranyl diphosphate = diphosphate + sabinene;
CC Xref=Rhea:RHEA:68636, ChEBI:CHEBI:33019, ChEBI:CHEBI:50027,
CC ChEBI:CHEBI:58057; Evidence={ECO:0000269|PubMed:17611797};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:68637;
CC Evidence={ECO:0000269|PubMed:17611797};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(2E)-geranyl diphosphate = (E)-beta-ocimene + diphosphate;
CC Xref=Rhea:RHEA:32691, ChEBI:CHEBI:33019, ChEBI:CHEBI:58057,
CC ChEBI:CHEBI:64280; EC=4.2.3.106;
CC Evidence={ECO:0000269|PubMed:17611797};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:32692;
CC Evidence={ECO:0000269|PubMed:17611797};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(2E)-geranyl diphosphate = beta-myrcene + diphosphate;
CC Xref=Rhea:RHEA:16965, ChEBI:CHEBI:17221, ChEBI:CHEBI:33019,
CC ChEBI:CHEBI:58057; EC=4.2.3.15;
CC Evidence={ECO:0000269|PubMed:17611797};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:16966;
CC Evidence={ECO:0000269|PubMed:17611797};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(2E)-geranyl diphosphate = alpha-pinene + diphosphate;
CC Xref=Rhea:RHEA:25662, ChEBI:CHEBI:33019, ChEBI:CHEBI:36740,
CC ChEBI:CHEBI:58057; Evidence={ECO:0000269|PubMed:17611797};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:25663;
CC Evidence={ECO:0000269|PubMed:17611797};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(2E)-geranyl diphosphate + H2O = (S)-alpha-terpineol +
CC diphosphate; Xref=Rhea:RHEA:32551, ChEBI:CHEBI:128,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:33019, ChEBI:CHEBI:58057;
CC EC=4.2.3.111; Evidence={ECO:0000269|PubMed:17611797};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:32552;
CC Evidence={ECO:0000269|PubMed:17611797};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000269|PubMed:17611797};
CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC Evidence={ECO:0000269|PubMed:17611797};
CC Note=Binds 3 Mg(2+) or Mn(2+) ions per subunit.
CC {ECO:0000269|PubMed:17611797};
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=1.1 uM for (2E)-geranyl diphosphate {ECO:0000269|PubMed:17611797};
CC Note=kcat is 0.33 sec(-1) with (2E)-geranyl diphosphate as substrate.
CC {ECO:0000269|PubMed:17611797};
CC pH dependence:
CC Optimum pH is 8. {ECO:0000269|PubMed:17611797};
CC Temperature dependence:
CC Optimum temperature is 35 degrees Celsius.
CC {ECO:0000269|PubMed:17611797};
CC -!- PATHWAY: Secondary metabolite biosynthesis; terpenoid biosynthesis.
CC {ECO:0000269|PubMed:17611797}.
CC -!- SUBUNIT: Monomer. {ECO:0000250|UniProtKB:Q6JD73}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000255}.
CC -!- TISSUE SPECIFICITY: Confined to buds and flowers.
CC {ECO:0000269|PubMed:17611797}.
CC -!- DEVELOPMENTAL STAGE: In flowers, restricted to stigma and style tissues
CC as well as in petals, primarily in the adaxial and abaxial epidermis
CC (PubMed:17611797). Barely detected in leaves, shoots, roots and sepals
CC (PubMed:17611797). {ECO:0000269|PubMed:17611797}.
CC -!- INDUCTION: In petals, transcripts, protein and enzyme activity levels
CC follow a circadian oscillation, with an increase during afternoon to
CC reach a maxima in darkness and exhibit lower levels in the morning,
CC thus leading to a nocturnal emission of floral monoterpenes.
CC {ECO:0000269|PubMed:17611797}.
CC -!- DOMAIN: The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important for
CC the catalytic activity, presumably through binding to Mg(2+).
CC {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the terpene synthase family. Tpsb subfamily.
CC {ECO:0000305}.
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DR EMBL; EF175166; ABP88782.1; -; mRNA.
DR BRENDA; 4.2.3.108; 10874.
DR UniPathway; UPA00213; -.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0034768; F:(E)-beta-ocimene synthase activity; IDA:UniProtKB.
DR GO; GO:0102313; F:1,8-cineole synthase activity; IDA:UniProtKB.
DR GO; GO:0000287; F:magnesium ion binding; IEA:InterPro.
DR GO; GO:0050551; F:myrcene synthase activity; IDA:UniProtKB.
DR GO; GO:0080015; F:sabinene synthase activity; IDA:UniProtKB.
DR GO; GO:0046248; P:alpha-pinene biosynthetic process; IDA:UniProtKB.
DR GO; GO:0007623; P:circadian rhythm; IEP:UniProtKB.
DR GO; GO:0016102; P:diterpenoid biosynthetic process; IEA:InterPro.
DR GO; GO:0010597; P:green leaf volatile biosynthetic process; IDA:UniProtKB.
DR GO; GO:0046250; P:limonene biosynthetic process; IDA:UniProtKB.
DR GO; GO:0043693; P:monoterpene biosynthetic process; IDA:UniProtKB.
DR CDD; cd00684; Terpene_cyclase_plant_C1; 1.
DR Gene3D; 1.10.600.10; -; 1.
DR Gene3D; 1.50.10.130; -; 1.
DR InterPro; IPR008949; Isoprenoid_synthase_dom_sf.
DR InterPro; IPR034741; Terpene_cyclase-like_1_C.
DR InterPro; IPR044814; Terpene_cyclase_plant_C1.
DR InterPro; IPR001906; Terpene_synth_N.
DR InterPro; IPR036965; Terpene_synth_N_sf.
DR InterPro; IPR005630; Terpene_synthase_metal-bd.
DR InterPro; IPR008930; Terpenoid_cyclase/PrenylTrfase.
DR Pfam; PF01397; Terpene_synth; 1.
DR Pfam; PF03936; Terpene_synth_C; 1.
DR SFLD; SFLDG01019; Terpene_Cyclase_Like_1_C_Termi; 1.
DR SUPFAM; SSF48239; SSF48239; 1.
DR SUPFAM; SSF48576; SSF48576; 1.
PE 1: Evidence at protein level;
KW Chloroplast; Lyase; Magnesium; Metal-binding; Plastid; Transit peptide.
FT TRANSIT 1..51
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 52..610
FT /note="1,8-cineol synthase, chloroplastic"
FT /id="PRO_5002688202"
FT MOTIF 359..363
FT /note="DDXXD motif"
FT /evidence="ECO:0000305"
FT BINDING 359
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="1"
FT /evidence="ECO:0000250|UniProtKB:Q40577"
FT BINDING 359
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="2"
FT /evidence="ECO:0000250|UniProtKB:Q40577"
FT BINDING 363
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="1"
FT /evidence="ECO:0000250|UniProtKB:Q40577"
FT BINDING 363
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="2"
FT /evidence="ECO:0000250|UniProtKB:Q40577"
FT BINDING 503
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="3"
FT /evidence="ECO:0000250|UniProtKB:Q40577"
FT BINDING 507
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="3"
FT /evidence="ECO:0000250|UniProtKB:Q40577"
FT BINDING 511
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="3"
FT /evidence="ECO:0000250|UniProtKB:Q40577"
SQ SEQUENCE 610 AA; 71146 MW; 60A66C301B894462 CRC64;
MNHHLIITPI FHLQIMLPVA TLKRPPPPAA TCSIYSFSRG TPSLVSKARL STAAVGGMKN
EPSPNHYSDI SSSDLNLTRR SGNYGPTMWD FEYIQSIHND YTEKKYMNRL NKLKEEMKKM
IMAEGSQELE KLELIDNLQR LGVSYHFKHE IMQILSSIKQ HSTPADSLYA TALKFRLFRE
YGFHISQEIF GGLSETHTED TKGMLYLYEA SFLATEGESE LEKARNWTEK HLREYLENKN
DDQNVAELVH HALELPLHWR MLRIEARWFI NFYKKKQDMI PLLLELAILD FNIVQAAHIE
DLKYVARWWK ETCLAENLPF ARDRLVENFF WTIGVNFLPQ YGYFRRIETK VNALVTTIDD
VYDVFGTMDE LQCFTHAFQR WNIDELDNLP DNMKMCYFAL DNFINEVAGD AFEEHRVPIL
SYLRNAWTDL CKAYLREAKW YFSKYIPTME EYMDNAWISI SAPVILVHAY FLVANPVNKE
VLHYLENYHD IIRWSALILR LANDLGTSSE ELKRGDVPKS IQCYMNEKKV SEEEARQHIR
LLISETWKKL NEAHNVAAHP FPKMFVKCAM NLARMAQCMY QHGDGHGHGD LNSETTNHIM
ALLFESIPPA
