ID   CION_CIOIN              Reviewed;         128 AA.
AC   P16240;
DT   01-APR-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1994, sequence version 2.
DT   25-MAY-2022, entry version 86.
DE   RecName: Full=Cionin;
DE   Flags: Precursor;
OS   Ciona intestinalis (Transparent sea squirt) (Ascidia intestinalis).
OC   Eukaryota; Metazoa; Chordata; Tunicata; Ascidiacea; Phlebobranchia;
OC   Cionidae; Ciona.
OX   NCBI_TaxID=7719;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=8405412; DOI=10.1016/0014-5793(93)80297-8;
RA   Monstein H.J., Thorup J.U., Folkesson R., Johnsen A.H., Rehfeld J.F.;
RT   "cDNA deduced procionin. Structure and expression in protochordates
RT   resemble that of procholecystokinin in mammals.";
RL   FEBS Lett. 331:60-64(1993).
RN   [2]
RP   PROTEIN SEQUENCE OF 109-116, SULFATION AT TYR-110 AND TYR-111, AND
RP   AMIDATION AT PHE-116.
RC   TISSUE=Neural ganglion;
RX   PubMed=2303439; DOI=10.1016/s0021-9258(19)39732-7;
RA   Johnsen A.H., Rehfeld J.F.;
RT   "Cionin: a disulfotyrosyl hybrid of cholecystokinin and gastrin from the
RT   neural ganglion of the protochordate Ciona intestinalis.";
RL   J. Biol. Chem. 265:3054-3058(1990).
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- TISSUE SPECIFICITY: Expressed in both the gut and the neural ganglion.
CC   -!- SIMILARITY: Belongs to the gastrin/cholecystokinin family.
CC       {ECO:0000305}.
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DR   EMBL; X69130; CAA48884.1; -; mRNA.
DR   PIR; S36901; S36901.
DR   RefSeq; NP_001027711.1; NM_001032539.1.
DR   AlphaFoldDB; P16240; -.
DR   GeneID; 445737; -.
DR   Proteomes; UP000008144; Unassembled WGS sequence.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0005179; F:hormone activity; IEA:UniProtKB-KW.
DR   GO; GO:0007218; P:neuropeptide signaling pathway; IEA:UniProtKB-KW.
DR   InterPro; IPR039236; GAST.
DR   InterPro; IPR001651; Gastrin/CCK.
DR   InterPro; IPR013152; Gastrin/cholecystokinin_CS.
DR   PANTHER; PTHR19309; PTHR19309; 1.
DR   Pfam; PF00918; Gastrin; 1.
DR   PROSITE; PS00259; GASTRIN; 1.
PE   1: Evidence at protein level;
KW   Amidation; Cleavage on pair of basic residues; Direct protein sequencing;
KW   Hormone; Neuropeptide; Reference proteome; Secreted; Signal; Sulfation.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000255"
FT   PROPEP          23..108
FT                   /evidence="ECO:0000269|PubMed:2303439"
FT                   /id="PRO_0000010671"
FT   PEPTIDE         109..116
FT                   /note="Cionin"
FT                   /id="PRO_0000010672"
FT   PROPEP          120..128
FT                   /id="PRO_0000010673"
FT   MOD_RES         110
FT                   /note="Sulfotyrosine"
FT                   /evidence="ECO:0000269|PubMed:2303439"
FT   MOD_RES         111
FT                   /note="Sulfotyrosine"
FT                   /evidence="ECO:0000269|PubMed:2303439"
FT   MOD_RES         116
FT                   /note="Phenylalanine amide"
FT                   /evidence="ECO:0000269|PubMed:2303439"
SQ   SEQUENCE   128 AA;  14820 MW;  BF1DE73438E66609 CRC64;
     MGSNIVIYFS IIVIVTLNVN GVPASDLFKS VSQYHIPRSK VINKETVTKP LQFQRAICRL
     LQKLGEETFA RLSQSELEAK QLDLIKTCYQ ANSFGDNENQ GHMQRMDRNY YGWMDFGKRA
     IEDVDYEY