CIP2A_MOUSE
ID CIP2A_MOUSE Reviewed; 907 AA.
AC Q8BWY9; B2RQ25; Q69ZH6; Q7TPZ7;
DT 15-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 27-JUL-2011, sequence version 3.
DT 03-AUG-2022, entry version 123.
DE RecName: Full=Protein CIP2A;
DE AltName: Full=Cancerous inhibitor of PP2A;
DE AltName: Full=p90 autoantigen homolog;
GN Name=Cip2a; Synonyms=Kiaa1524;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Egg, and Thymus;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 59-907.
RC TISSUE=Embryonic tail;
RX PubMed=15368895; DOI=10.1093/dnares/11.3.205;
RA Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S.,
RA Saga Y., Seino S., Nishimura M., Kaisho T., Hoshino K., Kitamura H.,
RA Nagase T., Ohara O., Koga H.;
RT "Prediction of the coding sequences of mouse homologues of KIAA gene: IV.
RT The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs
RT identified by screening of terminal sequences of cDNA clones randomly
RT sampled from size-fractionated libraries.";
RL DNA Res. 11:205-218(2004).
RN [4]
RP TISSUE SPECIFICITY.
RX PubMed=12118381; DOI=10.1038/sj.onc.1205625;
RA Soo Hoo L., Zhang J.Y., Chan E.K.L.;
RT "Cloning and characterization of a novel 90 kDa 'companion' auto-antigen of
RT p62 overexpressed in cancer.";
RL Oncogene 21:5006-5015(2002).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Promotes anchorage-independent cell growth and tumor
CC formation. {ECO:0000250|UniProtKB:Q8TCG1}.
CC -!- SUBUNIT: Homodimer. Interacts with MYC. Interacts with PPP2R5C; this
CC interaction stabilizes CIP2A. Interacts with PPP2R1A; this interaction
CC stabilizes CIP2A. {ECO:0000250|UniProtKB:Q8TCG1}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass membrane
CC protein {ECO:0000305}. Cytoplasm {ECO:0000250|UniProtKB:Q8TCG1}.
CC Note=Slightly concentrates in the perinuclear region.
CC {ECO:0000250|UniProtKB:Q8TCG1}.
CC -!- TISSUE SPECIFICITY: Highly expressed in 17.5 and 18.5 dpc liver but not
CC expressed in adult liver. Expressed at moderate level in embryonic
CC brain, muscle and epidermal layers. {ECO:0000269|PubMed:12118381}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAC33705.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AK049356; BAC33705.1; ALT_INIT; mRNA.
DR EMBL; BC137719; AAI37720.1; -; mRNA.
DR EMBL; BC052662; AAH52662.1; -; mRNA.
DR EMBL; AK173190; BAD32468.1; -; mRNA.
DR CCDS; CCDS49867.1; -.
DR RefSeq; NP_766204.2; NM_172616.2.
DR AlphaFoldDB; Q8BWY9; -.
DR SMR; Q8BWY9; -.
DR BioGRID; 230256; 1.
DR IntAct; Q8BWY9; 1.
DR STRING; 10090.ENSMUSP00000113075; -.
DR iPTMnet; Q8BWY9; -.
DR PhosphoSitePlus; Q8BWY9; -.
DR EPD; Q8BWY9; -.
DR MaxQB; Q8BWY9; -.
DR PaxDb; Q8BWY9; -.
DR PRIDE; Q8BWY9; -.
DR ProteomicsDB; 281471; -.
DR Antibodypedia; 32380; 270 antibodies from 22 providers.
DR DNASU; 224171; -.
DR Ensembl; ENSMUST00000048374; ENSMUSP00000044714; ENSMUSG00000033031.
DR GeneID; 224171; -.
DR KEGG; mmu:224171; -.
DR UCSC; uc007zkb.1; mouse.
DR CTD; 57650; -.
DR MGI; MGI:2146335; Cip2a.
DR VEuPathDB; HostDB:ENSMUSG00000033031; -.
DR eggNOG; ENOG502QTAP; Eukaryota.
DR GeneTree; ENSGT00940000153251; -.
DR HOGENOM; CLU_014754_0_0_1; -.
DR InParanoid; Q8BWY9; -.
DR OMA; AYKMLII; -.
DR OrthoDB; 188665at2759; -.
DR BioGRID-ORCS; 224171; 16 hits in 77 CRISPR screens.
DR ChiTaRS; Cip2a; mouse.
DR PRO; PR:Q8BWY9; -.
DR Proteomes; UP000000589; Chromosome 16.
DR RNAct; Q8BWY9; protein.
DR Bgee; ENSMUSG00000033031; Expressed in pharyngeal arch 2 and 184 other tissues.
DR ExpressionAtlas; Q8BWY9; baseline and differential.
DR Genevisible; Q8BWY9; MM.
DR GO; GO:0005737; C:cytoplasm; ISO:MGI.
DR GO; GO:0005829; C:cytosol; ISO:MGI.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; ISO:MGI.
DR GO; GO:0042803; F:protein homodimerization activity; ISS:UniProtKB.
DR GO; GO:0061351; P:neural precursor cell proliferation; IMP:MGI.
DR GO; GO:0007405; P:neuroblast proliferation; IMP:MGI.
DR GO; GO:0002052; P:positive regulation of neuroblast proliferation; IMP:MGI.
DR GO; GO:0007283; P:spermatogenesis; IMP:MGI.
DR Gene3D; 1.25.10.10; -; 1.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR042510; CIP2A.
DR PANTHER; PTHR23161; PTHR23161; 1.
DR SUPFAM; SSF48371; SSF48371; 1.
PE 1: Evidence at protein level;
KW Acetylation; Coiled coil; Cytoplasm; Membrane; Phosphoprotein;
KW Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..907
FT /note="Protein CIP2A"
FT /id="PRO_0000287142"
FT TRANSMEM 194..214
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 161..247
FT /note="Required for interaction with PPP2R5C"
FT /evidence="ECO:0000250|UniProtKB:Q8TCG1"
FT REGION 389..559
FT /note="Required for homodimerization"
FT /evidence="ECO:0000250|UniProtKB:Q8TCG1"
FT COILED 639..887
FT /evidence="ECO:0000255"
FT MOD_RES 1
FT /note="N-acetylmethionine"
FT /evidence="ECO:0000250|UniProtKB:Q8TCG1"
FT MOD_RES 906
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q8TCG1"
FT CONFLICT 216
FT /note="S -> R (in Ref. 1; BAC33705)"
FT /evidence="ECO:0000305"
FT CONFLICT 413..415
FT /note="KCE -> EVI (in Ref. 2; AAH52662)"
FT /evidence="ECO:0000305"
FT CONFLICT 699
FT /note="Q -> K (in Ref. 1; BAC33705)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 907 AA; 102043 MW; C4DA225F0492693B CRC64;
MDSTACLKSL LLSISQYKAV KSEANATQLL RHLEVVSGQK LTRLFTSHQI LPSECLSCLV
ELLEDPNISA SLILSIISLL SQLAIDNETR DCLQNIYNLN SVLSGVVCRS SACHNDSVFL
QCIQLLQRLT YNAKFFHSGA HIDDLITFLI GHVQSSEDEL TMPCLGLLAN LCRHNLSVQT
QIKTLSNVKS FYRTLISFLA HSSLTVVVFA LSILSSLTLN EEVGEKLFHA RNIHQTFQLI
FNILINGDGT LTRKYSVDLL MDLLKNPKIA DYLTRYEHFS SCLSQVLGLL NAKDPDSSSK
VLELLLAFCA VTQLRHVLSQ MMFEQSPSGN ILGSRPKSLE PTAALLRWLS QPLDGAENCS
VLALELFKEI FEDVIDTGNC SSTDHFVTLL LPTILDQLQF KEQNLDETLV RNKCERMVKA
IEVLLTLCGD DSLKMHVVKI LTTLKCTTLI EQQFTYGKID LGFGTKVADS ELCKLAADVI
LKTLTLMNKL KQLVPGMEVS FYKILQDPRL ITPLAFALTS DNREQVQSGL GILLEASPLP
DFPAFVLGES IAANNVYRQQ ETEHLPRKMT FQPLNHGFST SAKCLTPPPS KDNAPALNIE
DLIEKLQAGV MVKDQISDIR ISDIMDVYEM KLSTLASKES RLQDLLEAKA LALAQADRLI
AQYRCQRTQA ETEARTLAGM LREVERKNEE LSVLLKSQQL ESERAQNDIE HLFQHSKKLE
SVAAEHEILT KSYMELVQRN EATEKKNTDL QTTCESLNKH IETMKKLNEA LKQQNEKTIA
QLIEKEEQRK EVQSQLADRE CKLSNLHKIA KSQEEKLNVL QKEKEDKQET IDILRKELSR
TEQIRKELSI KASSLEMHKA QLEGRLEEKE SLLKLQQEEL NKHSHMIAMI HSLSGGKISP
ETVNLSI
