CIPC_HUMAN
ID CIPC_HUMAN Reviewed; 399 AA.
AC Q9C0C6; B2RCI1; Q8N389; Q8NDZ1;
DT 31-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT 31-OCT-2006, sequence version 2.
DT 03-AUG-2022, entry version 120.
DE RecName: Full=CLOCK-interacting pacemaker;
DE AltName: Full=CLOCK-interacting circadian protein;
GN Name=CIPC; Synonyms=KIAA1737;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RX PubMed=11214970; DOI=10.1093/dnares/7.6.347;
RA Nagase T., Kikuno R., Hattori A., Kondo Y., Okumura K., Ohara O.;
RT "Prediction of the coding sequences of unidentified human genes. XIX. The
RT complete sequences of 100 new cDNA clones from brain which code for large
RT proteins in vitro.";
RL DNA Res. 7:347-355(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Trachea;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Melanoma;
RX PubMed=17974005; DOI=10.1186/1471-2164-8-399;
RA Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U.,
RA Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D.,
RA Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A.,
RA Wiemann S., Schupp I.;
RT "The full-ORF clone resource of the German cDNA consortium.";
RL BMC Genomics 8:399-399(2007).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=12508121; DOI=10.1038/nature01348;
RA Heilig R., Eckenberg R., Petit J.-L., Fonknechten N., Da Silva C.,
RA Cattolico L., Levy M., Barbe V., De Berardinis V., Ureta-Vidal A.,
RA Pelletier E., Vico V., Anthouard V., Rowen L., Madan A., Qin S., Sun H.,
RA Du H., Pepin K., Artiguenave F., Robert C., Cruaud C., Bruels T.,
RA Jaillon O., Friedlander L., Samson G., Brottier P., Cure S., Segurens B.,
RA Aniere F., Samain S., Crespeau H., Abbasi N., Aiach N., Boscus D.,
RA Dickhoff R., Dors M., Dubois I., Friedman C., Gouyvenoux M., James R.,
RA Madan A., Mairey-Estrada B., Mangenot S., Martins N., Menard M., Oztas S.,
RA Ratcliffe A., Shaffer T., Trask B., Vacherie B., Bellemere C., Belser C.,
RA Besnard-Gonnet M., Bartol-Mavel D., Boutard M., Briez-Silla S.,
RA Combette S., Dufosse-Laurent V., Ferron C., Lechaplais C., Louesse C.,
RA Muselet D., Magdelenat G., Pateau E., Petit E., Sirvain-Trukniewicz P.,
RA Trybou A., Vega-Czarny N., Bataille E., Bluet E., Bordelais I., Dubois M.,
RA Dumont C., Guerin T., Haffray S., Hammadi R., Muanga J., Pellouin V.,
RA Robert D., Wunderle E., Gauguet G., Roy A., Sainte-Marthe L., Verdier J.,
RA Verdier-Discala C., Hillier L.W., Fulton L., McPherson J., Matsuda F.,
RA Wilson R., Scarpelli C., Gyapay G., Wincker P., Saurin W., Quetier F.,
RA Waterston R., Hood L., Weissenbach J.;
RT "The DNA sequence and analysis of human chromosome 14.";
RL Nature 421:601-607(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND VARIANT PHE-31.
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [7]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-213, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma, and Erythroleukemia;
RX PubMed=23186163; DOI=10.1021/pr300630k;
RA Zhou H., Di Palma S., Preisinger C., Peng M., Polat A.N., Heck A.J.,
RA Mohammed S.;
RT "Toward a comprehensive characterization of a human cancer cell
RT phosphoproteome.";
RL J. Proteome Res. 12:260-271(2013).
RN [8]
RP SUBCELLULAR LOCATION, INTERACTION WITH CAD AND HSPA5, AND MUTAGENESIS OF
RP LYS-186 AND LYS-187.
RX PubMed=26657846; DOI=10.1016/j.bbrc.2015.11.117;
RA Matsunaga R., Nishino T., Yokoyama A., Nakashima A., Kikkawa U.,
RA Konishi H.;
RT "Versatile function of the circadian protein CIPC as a regulator of Erk
RT activation.";
RL Biochem. Biophys. Res. Commun. 469:377-383(2016).
CC -!- FUNCTION: Transcriptional repressor which may act as a negative-
CC feedback regulator of CLOCK-ARNTL/BMAL1 transcriptional activity in the
CC circadian-clock mechanism. May stimulate ARNTL/BMAL1-dependent
CC phosphorylation of CLOCK. However, the physiogical relevance of these
CC observations is unsure, since experiments in an animal model showed
CC that CIPC is not critially required for basic circadian clock.
CC {ECO:0000250|UniProtKB:Q8R0W1}.
CC -!- SUBUNIT: Interacts with CLOCK. Forms a ternary complex with the CLOCK-
CC ARNTL/BMAL1 heterodimer. Interacts with CAD AND HSPA5
CC (PubMed:26657846). {ECO:0000250|UniProtKB:Q8R0W1,
CC ECO:0000269|PubMed:26657846}.
CC -!- INTERACTION:
CC Q9C0C6; O43639: NCK2; NbExp=3; IntAct=EBI-5654244, EBI-713635;
CC Q9C0C6; Q9UJ41: RABGEF1; NbExp=3; IntAct=EBI-5654244, EBI-913954;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:26657846}. Cytoplasm,
CC cytosol {ECO:0000269|PubMed:26657846}. Note=Predominantly localizes to
CC the nucleus, where it co-localizes with CLOCK. At the G1/S boundary,
CC partially translocated to the cytosol. {ECO:0000250|UniProtKB:Q8R0W1,
CC ECO:0000269|PubMed:26657846}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB21828.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AB051524; BAB21828.1; ALT_INIT; mRNA.
DR EMBL; AK315124; BAG37578.1; -; mRNA.
DR EMBL; AL834514; CAD39170.1; -; mRNA.
DR EMBL; AC007686; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471061; EAW81270.1; -; Genomic_DNA.
DR EMBL; BC037300; AAH37300.1; -; mRNA.
DR CCDS; CCDS9855.1; -.
DR RefSeq; NP_219494.2; NM_033426.2.
DR AlphaFoldDB; Q9C0C6; -.
DR SMR; Q9C0C6; -.
DR BioGRID; 124541; 16.
DR IntAct; Q9C0C6; 8.
DR STRING; 9606.ENSP00000355319; -.
DR iPTMnet; Q9C0C6; -.
DR PhosphoSitePlus; Q9C0C6; -.
DR BioMuta; CIPC; -.
DR DMDM; 117949601; -.
DR jPOST; Q9C0C6; -.
DR MassIVE; Q9C0C6; -.
DR MaxQB; Q9C0C6; -.
DR PaxDb; Q9C0C6; -.
DR PeptideAtlas; Q9C0C6; -.
DR PRIDE; Q9C0C6; -.
DR ProteomicsDB; 79998; -.
DR Antibodypedia; 99; 40 antibodies from 9 providers.
DR DNASU; 85457; -.
DR Ensembl; ENST00000361786.7; ENSP00000355319.2; ENSG00000198894.8.
DR GeneID; 85457; -.
DR KEGG; hsa:85457; -.
DR MANE-Select; ENST00000361786.7; ENSP00000355319.2; NM_033426.3; NP_219494.2.
DR UCSC; uc001xtd.4; human.
DR CTD; 85457; -.
DR GeneCards; CIPC; -.
DR HGNC; HGNC:20365; CIPC.
DR HPA; ENSG00000198894; Tissue enhanced (skeletal).
DR MIM; 616995; gene.
DR neXtProt; NX_Q9C0C6; -.
DR OpenTargets; ENSG00000198894; -.
DR PharmGKB; PA134877961; -.
DR VEuPathDB; HostDB:ENSG00000198894; -.
DR eggNOG; ENOG502RJ2N; Eukaryota.
DR GeneTree; ENSGT00510000048522; -.
DR HOGENOM; CLU_056483_0_0_1; -.
DR InParanoid; Q9C0C6; -.
DR OMA; PHPVGKK; -.
DR OrthoDB; 1183035at2759; -.
DR PhylomeDB; Q9C0C6; -.
DR TreeFam; TF332579; -.
DR PathwayCommons; Q9C0C6; -.
DR SignaLink; Q9C0C6; -.
DR BioGRID-ORCS; 85457; 12 hits in 1081 CRISPR screens.
DR ChiTaRS; CIPC; human.
DR GenomeRNAi; 85457; -.
DR Pharos; Q9C0C6; Tdark.
DR PRO; PR:Q9C0C6; -.
DR Proteomes; UP000005640; Chromosome 14.
DR RNAct; Q9C0C6; protein.
DR Bgee; ENSG00000198894; Expressed in skeletal muscle tissue of rectus abdominis and 190 other tissues.
DR ExpressionAtlas; Q9C0C6; baseline and differential.
DR Genevisible; Q9C0C6; HS.
DR GO; GO:0005829; C:cytosol; IDA:HPA.
DR GO; GO:0005730; C:nucleolus; IDA:HPA.
DR GO; GO:0005654; C:nucleoplasm; IDA:HPA.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0042754; P:negative regulation of circadian rhythm; ISS:UniProtKB.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; ISS:UniProtKB.
DR GO; GO:0048511; P:rhythmic process; IEA:UniProtKB-KW.
DR InterPro; IPR031602; CIPC.
DR PANTHER; PTHR34648; PTHR34648; 1.
DR Pfam; PF15800; CiPC; 1.
PE 1: Evidence at protein level;
KW Biological rhythms; Coiled coil; Cytoplasm; Nucleus; Phosphoprotein;
KW Reference proteome; Repressor; Transcription; Transcription regulation.
FT CHAIN 1..399
FT /note="CLOCK-interacting pacemaker"
FT /id="PRO_0000256133"
FT REGION 1..85
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 162..224
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 378..399
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 333..359
FT /evidence="ECO:0000255"
FT MOTIF 186..187
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000305|PubMed:26657846"
FT COMPBIAS 1..24
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 45..60
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 192..210
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 213
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:23186163"
FT VARIANT 31
FT /note="L -> F (in dbSNP:rs11552006)"
FT /evidence="ECO:0000269|PubMed:15489334"
FT /id="VAR_028881"
FT VARIANT 203
FT /note="T -> A (in dbSNP:rs759593)"
FT /id="VAR_049526"
FT MUTAGEN 186
FT /note="K->A: Loss of predominant localization in the
FT nucleus; when associated with A-187. No effect on CAD- AND
FT HSPA5-binding; when associated with A-187."
FT /evidence="ECO:0000269|PubMed:26657846"
FT MUTAGEN 187
FT /note="K->A: Loss of predominant localization in the
FT nucleus; when associated with A-186. No effect on CAD- AND
FT HSPA5-binding; when associated with A-186."
FT /evidence="ECO:0000269|PubMed:26657846"
SQ SEQUENCE 399 AA; 42692 MW; A02AE8F6543781D9 CRC64;
MERKNPSRES PRRLSAKVGK GTEMKKVARQ LGMAAAESDK DSGFSDGSSE CLSSAEQMES
EDMLSALGWS REDRPRQNSK TAKNAFPTLS PMVVMKNVLV KQGSSSSQLQ SWTVQPSFEV
ISAQPQLLFL HPPVPSPVSP CHTGEKKSDS RNYLPILNSY TKIAPHPGKR GLSLGPEEKG
TSGVQKKICT ERLGPSLSSS EPTKAGAVPS SPSTPAPPSA KLAEDSALQG VPSLVAGGSP
QTLQPVSSSH VAKAPSLTFA SPASPVCASD STLHGLESNS PLSPLSANYS SPLWAAEHLC
RSPDIFSEQR QSKHRRFQNT LVVLHKSGLL EITLKTKELI RQNQATQVEL DQLKEQTQLF
IEATKSRAPQ AWAKLQASLT PGSSNTGSDL EAFSDHPAI
