CIRA_ECOLI
ID CIRA_ECOLI Reviewed; 663 AA.
AC P17315; Q2MAS4;
DT 01-AUG-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 2.
DT 03-AUG-2022, entry version 175.
DE RecName: Full=Colicin I receptor;
DE Flags: Precursor;
GN Name=cirA; Synonyms=cir, feuA; OrderedLocusNames=b2155, JW2142;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2644220; DOI=10.1128/jb.171.2.1041-1047.1989;
RA Nau C.D., Konisky J.;
RT "Evolutionary relationship between the TonB-dependent outer membrane
RT transport proteins: nucleotide and amino acid sequences of the Escherichia
RT coli colicin I receptor gene.";
RL J. Bacteriol. 171:1041-1047(1989).
RN [2]
RP ERRATUM OF PUBMED:2644220, AND SEQUENCE REVISION.
RA Nau C.D., Konisky J.;
RL J. Bacteriol. 171:4530-4530(1989).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / BHB2600;
RA Richterich P., Lakey N., Gryan G., Jaehn L., Mintz L., Robison K.,
RA Church G.M.;
RT "Automated multiplex sequencing of the E.coli genome.";
RL Submitted (OCT-1993) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [6]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-59, AND PROTEIN SEQUENCE OF 26-46.
RX PubMed=3316180; DOI=10.1128/jb.169.12.5343-5352.1987;
RA Griggs D.W., Tharp B.B., Konisky J.;
RT "Cloning and promoter identification of the iron-regulated cir gene of
RT Escherichia coli.";
RL J. Bacteriol. 169:5343-5352(1987).
RN [7]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-125.
RC STRAIN=BPR2;
RX PubMed=1315732; DOI=10.1128/jb.174.10.3242-3249.1992;
RA Steffes C., Ellis J., Wu J., Rosen B.P.;
RT "The lysP gene encodes the lysine-specific permease.";
RL J. Bacteriol. 174:3242-3249(1992).
RN [8]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-21.
RX PubMed=2160948; DOI=10.1128/jb.172.6.3529-3533.1990;
RA Griggs D.W., Kafka K., Nau C.D., Konisky J.;
RT "Activation of expression of the Escherichia coli cir gene by an iron-
RT independent regulatory mechanism involving cyclic AMP-cyclic AMP receptor
RT protein complex.";
RL J. Bacteriol. 172:3529-3533(1990).
RN [9]
RP IDENTIFICATION BY 2D-GEL.
RX PubMed=9298644; DOI=10.1002/elps.1150180805;
RA VanBogelen R.A., Abshire K.Z., Moldover B., Olson E.R., Neidhardt F.C.;
RT "Escherichia coli proteome analysis using the gene-protein database.";
RL Electrophoresis 18:1243-1251(1997).
CC -!- FUNCTION: Not yet known. Postulated to participate in iron transport.
CC Outer membrane receptor for colicins IA and IB.
CC -!- SUBCELLULAR LOCATION: Cell outer membrane.
CC -!- INDUCTION: By iron and by a cAMP/cAMP receptor protein complex.
CC -!- SIMILARITY: Belongs to the TonB-dependent receptor family.
CC {ECO:0000305}.
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DR EMBL; J04229; AAA75183.1; -; Genomic_DNA.
DR EMBL; U00007; AAA60531.1; -; Genomic_DNA.
DR EMBL; U00096; AAC75216.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE76632.1; -; Genomic_DNA.
DR EMBL; M19295; AAA23581.1; -; Genomic_DNA.
DR EMBL; M89774; AAA17054.1; -; Genomic_DNA.
DR PIR; B64984; QRECIC.
DR RefSeq; NP_416660.1; NC_000913.3.
DR RefSeq; WP_000489247.1; NZ_SSZK01000011.1.
DR PDB; 2HDF; X-ray; 2.65 A; A=26-663.
DR PDB; 2HDI; X-ray; 2.50 A; A=26-663.
DR PDBsum; 2HDF; -.
DR PDBsum; 2HDI; -.
DR AlphaFoldDB; P17315; -.
DR SMR; P17315; -.
DR BioGRID; 4260473; 99.
DR BioGRID; 853286; 1.
DR ComplexPortal; CPX-3577; Ferric-catecholate outer membrane transporter complex.
DR DIP; DIP-9282N; -.
DR IntAct; P17315; 7.
DR MINT; P17315; -.
DR STRING; 511145.b2155; -.
DR DrugBank; DB14879; Cefiderocol.
DR DrugBank; DB04147; Dodecyldimethylamine N-oxide.
DR DrugBank; DB02415; N-Octyl-2-Hydroxyethyl Sulfoxide.
DR TCDB; 1.B.14.1.4; the outer membrane receptor (omr) family.
DR SWISS-2DPAGE; P17315; -.
DR jPOST; P17315; -.
DR PaxDb; P17315; -.
DR PRIDE; P17315; -.
DR EnsemblBacteria; AAC75216; AAC75216; b2155.
DR EnsemblBacteria; BAE76632; BAE76632; BAE76632.
DR GeneID; 949042; -.
DR KEGG; ecj:JW2142; -.
DR KEGG; eco:b2155; -.
DR PATRIC; fig|1411691.4.peg.85; -.
DR EchoBASE; EB0153; -.
DR eggNOG; COG4771; Bacteria.
DR HOGENOM; CLU_008287_18_2_6; -.
DR InParanoid; P17315; -.
DR OMA; KAPDFRQ; -.
DR PhylomeDB; P17315; -.
DR BioCyc; EcoCyc:EG10155-MON; -.
DR EvolutionaryTrace; P17315; -.
DR PRO; PR:P17315; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0009279; C:cell outer membrane; IDA:EcoCyc.
DR GO; GO:0031230; C:intrinsic component of cell outer membrane; IBA:GO_Central.
DR GO; GO:0016020; C:membrane; IC:ComplexPortal.
DR GO; GO:1902495; C:transmembrane transporter complex; IC:ComplexPortal.
DR GO; GO:0042912; F:colicin transmembrane transporter activity; IDA:EcoCyc.
DR GO; GO:0015343; F:siderophore transmembrane transporter activity; IMP:EcoCyc.
DR GO; GO:0015344; F:siderophore uptake transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0055072; P:iron ion homeostasis; IC:ComplexPortal.
DR GO; GO:0044718; P:siderophore transmembrane transport; IMP:EcoCyc.
DR Gene3D; 2.170.130.10; -; 1.
DR Gene3D; 2.40.170.20; -; 1.
DR InterPro; IPR039426; BtuB-like.
DR InterPro; IPR012910; Plug_dom.
DR InterPro; IPR037066; Plug_dom_sf.
DR InterPro; IPR000531; TonB-dep_rcpt_b-brl.
DR InterPro; IPR010916; TonB_box_CS.
DR InterPro; IPR036942; TonB_rcpt_b-brl_sf.
DR InterPro; IPR010917; TonB_rcpt_CS.
DR PANTHER; PTHR30069; PTHR30069; 1.
DR Pfam; PF07715; Plug; 1.
DR Pfam; PF00593; TonB_dep_Rec; 1.
DR PROSITE; PS00430; TONB_DEPENDENT_REC_1; 1.
DR PROSITE; PS01156; TONB_DEPENDENT_REC_2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cell outer membrane; Direct protein sequencing;
KW Ion transport; Iron; Iron transport; Membrane; Receptor;
KW Reference proteome; Signal; TonB box; Transmembrane;
KW Transmembrane beta strand; Transport.
FT SIGNAL 1..25
FT /evidence="ECO:0000269|PubMed:3316180"
FT CHAIN 26..663
FT /note="Colicin I receptor"
FT /id="PRO_0000034744"
FT REGION 207..234
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 252..275
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 31..38
FT /note="TonB box"
FT MOTIF 646..663
FT /note="TonB C-terminal box"
FT COMPBIAS 255..275
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 97
FT /note="S -> D (in Ref. 7; AAA17054)"
FT /evidence="ECO:0000305"
FT CONFLICT 528
FT /note="I -> N (in Ref. 1; AAA75183)"
FT /evidence="ECO:0000305"
FT STRAND 33..40
FT /evidence="ECO:0007829|PDB:2HDI"
FT HELIX 44..46
FT /evidence="ECO:0007829|PDB:2HDI"
FT STRAND 48..54
FT /evidence="ECO:0007829|PDB:2HDI"
FT HELIX 56..60
FT /evidence="ECO:0007829|PDB:2HDI"
FT HELIX 68..71
FT /evidence="ECO:0007829|PDB:2HDI"
FT STRAND 77..81
FT /evidence="ECO:0007829|PDB:2HDI"
FT STRAND 83..85
FT /evidence="ECO:0007829|PDB:2HDI"
FT STRAND 87..91
FT /evidence="ECO:0007829|PDB:2HDI"
FT HELIX 96..98
FT /evidence="ECO:0007829|PDB:2HDI"
FT STRAND 99..103
FT /evidence="ECO:0007829|PDB:2HDI"
FT TURN 111..113
FT /evidence="ECO:0007829|PDB:2HDI"
FT HELIX 120..122
FT /evidence="ECO:0007829|PDB:2HDI"
FT STRAND 123..125
FT /evidence="ECO:0007829|PDB:2HDI"
FT HELIX 127..129
FT /evidence="ECO:0007829|PDB:2HDI"
FT STRAND 130..138
FT /evidence="ECO:0007829|PDB:2HDI"
FT HELIX 141..144
FT /evidence="ECO:0007829|PDB:2HDI"
FT TURN 146..149
FT /evidence="ECO:0007829|PDB:2HDI"
FT STRAND 150..157
FT /evidence="ECO:0007829|PDB:2HDI"
FT STRAND 165..177
FT /evidence="ECO:0007829|PDB:2HDI"
FT STRAND 183..195
FT /evidence="ECO:0007829|PDB:2HDI"
FT TURN 196..198
FT /evidence="ECO:0007829|PDB:2HDI"
FT STRAND 199..210
FT /evidence="ECO:0007829|PDB:2HDI"
FT STRAND 231..243
FT /evidence="ECO:0007829|PDB:2HDI"
FT STRAND 249..263
FT /evidence="ECO:0007829|PDB:2HDI"
FT STRAND 271..284
FT /evidence="ECO:0007829|PDB:2HDI"
FT STRAND 289..302
FT /evidence="ECO:0007829|PDB:2HDI"
FT STRAND 306..308
FT /evidence="ECO:0007829|PDB:2HDI"
FT STRAND 310..325
FT /evidence="ECO:0007829|PDB:2HDI"
FT HELIX 326..328
FT /evidence="ECO:0007829|PDB:2HDI"
FT STRAND 330..344
FT /evidence="ECO:0007829|PDB:2HDI"
FT HELIX 346..348
FT /evidence="ECO:0007829|PDB:2HDI"
FT STRAND 349..351
FT /evidence="ECO:0007829|PDB:2HDI"
FT TURN 353..355
FT /evidence="ECO:0007829|PDB:2HDI"
FT STRAND 357..373
FT /evidence="ECO:0007829|PDB:2HDI"
FT STRAND 376..387
FT /evidence="ECO:0007829|PDB:2HDI"
FT TURN 388..390
FT /evidence="ECO:0007829|PDB:2HDI"
FT STRAND 391..419
FT /evidence="ECO:0007829|PDB:2HDI"
FT HELIX 423..426
FT /evidence="ECO:0007829|PDB:2HDI"
FT STRAND 430..434
FT /evidence="ECO:0007829|PDB:2HDI"
FT HELIX 435..437
FT /evidence="ECO:0007829|PDB:2HDI"
FT STRAND 439..442
FT /evidence="ECO:0007829|PDB:2HDI"
FT STRAND 450..462
FT /evidence="ECO:0007829|PDB:2HDI"
FT HELIX 466..468
FT /evidence="ECO:0007829|PDB:2HDI"
FT STRAND 472..487
FT /evidence="ECO:0007829|PDB:2HDI"
FT STRAND 490..492
FT /evidence="ECO:0007829|PDB:2HDI"
FT TURN 494..496
FT /evidence="ECO:0007829|PDB:2HDI"
FT HELIX 497..499
FT /evidence="ECO:0007829|PDB:2HDI"
FT STRAND 503..508
FT /evidence="ECO:0007829|PDB:2HDI"
FT STRAND 512..519
FT /evidence="ECO:0007829|PDB:2HDI"
FT STRAND 521..538
FT /evidence="ECO:0007829|PDB:2HDI"
FT STRAND 540..556
FT /evidence="ECO:0007829|PDB:2HDI"
FT HELIX 558..560
FT /evidence="ECO:0007829|PDB:2HDF"
FT STRAND 563..579
FT /evidence="ECO:0007829|PDB:2HDI"
FT STRAND 582..585
FT /evidence="ECO:0007829|PDB:2HDF"
FT STRAND 587..596
FT /evidence="ECO:0007829|PDB:2HDI"
FT STRAND 612..624
FT /evidence="ECO:0007829|PDB:2HDI"
FT STRAND 627..635
FT /evidence="ECO:0007829|PDB:2HDI"
FT STRAND 654..663
FT /evidence="ECO:0007829|PDB:2HDI"
SQ SEQUENCE 663 AA; 73896 MW; 2C68A45D4B5EE414 CRC64;
MFRLNPFVRV GLCLSAISCA WPVLAVDDDG ETMVVTASSV EQNLKDAPAS ISVITQEDLQ
RKPVQNLKDV LKEVPGVQLT NEGDNRKGVS IRGLDSSYTL ILVDGKRVNS RNAVFRHNDF
DLNWIPVDSI ERIEVVRGPM SSLYGSDALG GVVNIITKKI GQKWSGTVTV DTTIQEHRDR
GDTYNGQFFT SGPLIDGVLG MKAYGSLAKR EKDDPQNSTT TDTGETPRIE GFSSRDGNVE
FAWTPNQNHD FTAGYGFDRQ DRDSDSLDKN RLERQNYSVS HNGRWDYGTS ELKYYGEKVE
NKNPGNSSPI TSESNTVDGK YTLPLTAINQ FLTVGGEWRH DKLSDAVNLT GGTSSKTSAS
QYALFVEDEW RIFEPLALTT GVRMDDHETY GEHWSPRAYL VYNATDTVTV KGGWATAFKA
PSLLQLSPDW TSNSCRGACK IVGSPDLKPE TSESWELGLY YMGEEGWLEG VESSVTVFRN
DVKDRISISR TSDVNAAPGY QNFVGFETGA NGRRIPVFSY YNVNKARIQG VETELKIPFN
DEWKLSINYT YNDGRDVSNG ENKPLSDLPF HTANGTLDWK PLALEDWSFY VSGHYTGQKR
ADSATAKTPG GYTIWNTGAA WQVTKDVKLR AGVLNLGDKD LSRDDYSYNE DGRRYFMAVD
YRF
