CISA_BACSU
ID CISA_BACSU Reviewed; 500 AA.
AC P17867;
DT 01-AUG-1990, integrated into UniProtKB/Swiss-Prot.
DT 07-JUL-2009, sequence version 2.
DT 03-AUG-2022, entry version 133.
DE RecName: Full=Putative DNA recombinase;
DE AltName: Full=Stage IV sporulation protein CA;
GN Name=cisA; Synonyms=spoIVCA; OrderedLocusNames=BSU25770;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2105293; DOI=10.1128/jb.172.2.1092-1098.1990;
RA Sato T., Samori Y., Kobayashi Y.;
RT "The cisA cistron of Bacillus subtilis sporulation gene spoIVC encodes a
RT protein homologous to a site-specific recombinase.";
RL J. Bacteriol. 172:1092-1098(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168 / JH642;
RX PubMed=7704261; DOI=10.1099/13500872-141-2-323;
RA Takemaru K., Mizuno M., Sato T., Takeuchi M., Kobayashi Y.;
RT "Complete nucleotide sequence of a skin element excised by DNA
RT rearrangement during sporulation in Bacillus subtilis.";
RL Microbiology 141:323-327(1995).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168 / JH642;
RX PubMed=8969508; DOI=10.1099/13500872-142-11-3103;
RA Mizuno M., Masuda S., Takemaru K., Hosono S., Sato T., Takeuchi M.,
RA Kobayashi Y.;
RT "Systematic sequencing of the 283 kb 210 degrees-232 degrees region of the
RT Bacillus subtilis genome containing the skin element and many sporulation
RT genes.";
RL Microbiology 142:3103-3111(1996).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [5]
RP SEQUENCE REVISION TO 438.
RX PubMed=19383706; DOI=10.1099/mic.0.027839-0;
RA Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A.,
RA Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.;
RT "From a consortium sequence to a unified sequence: the Bacillus subtilis
RT 168 reference genome a decade later.";
RL Microbiology 155:1758-1775(2009).
CC -!- FUNCTION: Putative site-specific recombinase having a very important
CC role in sporulation. It probably plays a role in the recombination of
CC SpoIIIC and SpoIVCB to form sigma K factor.
CC -!- SIMILARITY: In the N-terminal section; belongs to the site-specific
CC recombinase resolvase family. {ECO:0000305}.
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DR EMBL; M29040; AAA22314.1; -; Genomic_DNA.
DR EMBL; D32216; BAA06971.1; -; Genomic_DNA.
DR EMBL; D84432; BAA12435.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB14518.2; -; Genomic_DNA.
DR PIR; A43656; A43656.
DR RefSeq; NP_390454.2; NC_000964.3.
DR RefSeq; WP_010886573.1; NZ_JNCM01000036.1.
DR AlphaFoldDB; P17867; -.
DR SMR; P17867; -.
DR IntAct; P17867; 6.
DR STRING; 224308.BSU25770; -.
DR PaxDb; P17867; -.
DR PRIDE; P17867; -.
DR DNASU; 937799; -.
DR EnsemblBacteria; CAB14518; CAB14518; BSU_25770.
DR GeneID; 937799; -.
DR KEGG; bsu:BSU25770; -.
DR PATRIC; fig|224308.179.peg.2801; -.
DR eggNOG; COG1961; Bacteria.
DR InParanoid; P17867; -.
DR OMA; VVRQILM; -.
DR PhylomeDB; P17867; -.
DR BioCyc; BSUB:BSU25770-MON; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0000150; F:DNA strand exchange activity; IBA:GO_Central.
DR GO; GO:0015074; P:DNA integration; IEA:UniProtKB-KW.
DR GO; GO:0006310; P:DNA recombination; IBA:GO_Central.
DR Gene3D; 3.40.50.1390; -; 1.
DR Gene3D; 3.90.1750.20; -; 1.
DR InterPro; IPR038109; DNA_bind_recomb_sf.
DR InterPro; IPR011109; DNA_bind_recombinase_dom.
DR InterPro; IPR006118; Recombinase_CS.
DR InterPro; IPR006119; Resolv_N.
DR InterPro; IPR036162; Resolvase-like_N_sf.
DR InterPro; IPR025827; Zn_ribbon_recom_dom.
DR Pfam; PF07508; Recombinase; 1.
DR Pfam; PF00239; Resolvase; 1.
DR Pfam; PF13408; Zn_ribbon_recom; 1.
DR SMART; SM00857; Resolvase; 1.
DR SUPFAM; SSF53041; SSF53041; 1.
DR PROSITE; PS51737; RECOMBINASE_DNA_BIND; 1.
DR PROSITE; PS00397; RECOMBINASES_1; 1.
DR PROSITE; PS51736; RECOMBINASES_3; 1.
PE 3: Inferred from homology;
KW Coiled coil; DNA integration; DNA recombination; DNA-binding;
KW Reference proteome.
FT CHAIN 1..500
FT /note="Putative DNA recombinase"
FT /id="PRO_0000196383"
FT DOMAIN 1..144
FT /note="Resolvase/invertase-type recombinase catalytic"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01072"
FT DNA_BIND 152..288
FT /note="Recombinase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01073"
FT COILED 372..448
FT /evidence="ECO:0000255"
FT ACT_SITE 9
FT /note="O-(5'-phospho-DNA)-serine intermediate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01072"
FT CONFLICT 438
FT /note="E -> R (in Ref. 1; AAA22314, 2; BAA06971 and 3;
FT BAA12435)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 500 AA; 57455 MW; E925F89A4BC88F16 CRC64;
MIAIYVRVST EEQAIKGSSI DSQIEACIKK AGTKDVLKYA DEGFSGELLE RPALNRLRED
ASKGLISQVI CYDPDRLSRK LMNQLIIDDE LRKRNIPLIF VNGEYANSPE GQLFFAMRGA
ISEFEKAKIK ERTSSGRLQK MKKGMIIKDS KLYGYKFVKE KRTLEILEEE AKIIRMIFNY
FTDHKSPFFG RVNGIALHLT QMGVKTKKGA KVWHRQVVRQ ILMNSSYKGE HRQYKYDTEG
SYVSKQAGNK SIIKIRPEEE QITVTIPAIV PAEQWDYAQE LLGQSKRKHL SISPHNYLLS
GLVRCGKCGN TMTGKKRKSH GKDYYVYTCR KNYSGAKDRG CGKEMSENKL NRHVWGEIFK
FITNPQKYVS FKEAEQSNHL SDELELIEKE IEKTKKGRKR LLTLISLSDD DDLDIDEIKA
QIIELQKKQN QLTEKCNEIQ SKMKVLDDTS SSENALKRAI DYFQSIGADN LTLEDKKTIV
NFIVKEVTIV DSDTIYIETY
