CISY1_ARATH
ID CISY1_ARATH Reviewed; 480 AA.
AC Q9LXS7;
DT 30-AUG-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 135.
DE RecName: Full=Citrate synthase 1, peroxisomal;
DE EC=2.3.3.16;
DE Flags: Precursor;
GN Name=CSY1; OrderedLocusNames=At3g58740; ORFNames=T20N10.90;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RX PubMed=15923350; DOI=10.1105/tpc.105.031856;
RA Pracharoenwattana I., Cornah J.E., Smith S.M.;
RT "Arabidopsis peroxisomal citrate synthase is required for fatty acid
RT respiration and seed germination.";
RL Plant Cell 17:2037-2048(2005).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=acetyl-CoA + H2O + oxaloacetate = citrate + CoA + H(+);
CC Xref=Rhea:RHEA:16845, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:16452, ChEBI:CHEBI:16947, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:57288; EC=2.3.3.16; Evidence={ECO:0000255|PROSITE-
CC ProRule:PRU10117};
CC -!- PATHWAY: Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate
CC from oxaloacetate: step 1/2.
CC -!- SUBCELLULAR LOCATION: Peroxisome {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed only in siliques. Not expressed in
CC flower, stem, cauline leaf, young leaf, mature leaf and senescent leaf.
CC {ECO:0000269|PubMed:15923350}.
CC -!- DEVELOPMENTAL STAGE: Weakly or not expressed during seedling growth.
CC {ECO:0000269|PubMed:15923350}.
CC -!- MISCELLANEOUS: Citrate synthase is found in nearly all cells capable of
CC oxidative metabolism.
CC -!- SIMILARITY: Belongs to the citrate synthase family. {ECO:0000305}.
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DR EMBL; AL353032; CAB88291.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE79825.1; -; Genomic_DNA.
DR EMBL; AY099611; AAM20462.1; -; mRNA.
DR EMBL; BT002169; AAN72180.1; -; mRNA.
DR PIR; T49157; T49157.
DR RefSeq; NP_191433.1; NM_115736.2.
DR AlphaFoldDB; Q9LXS7; -.
DR SMR; Q9LXS7; -.
DR BioGRID; 10358; 21.
DR IntAct; Q9LXS7; 1.
DR STRING; 3702.AT3G58740.1; -.
DR iPTMnet; Q9LXS7; -.
DR PaxDb; Q9LXS7; -.
DR PRIDE; Q9LXS7; -.
DR ProteomicsDB; 246801; -.
DR EnsemblPlants; AT3G58740.1; AT3G58740.1; AT3G58740.
DR GeneID; 825043; -.
DR Gramene; AT3G58740.1; AT3G58740.1; AT3G58740.
DR KEGG; ath:AT3G58740; -.
DR Araport; AT3G58740; -.
DR TAIR; locus:2099089; AT3G58740.
DR eggNOG; KOG2617; Eukaryota.
DR HOGENOM; CLU_025068_0_1_1; -.
DR InParanoid; Q9LXS7; -.
DR OMA; DANPAHM; -.
DR OrthoDB; 691172at2759; -.
DR PhylomeDB; Q9LXS7; -.
DR BioCyc; ARA:AT3G58740-MON; -.
DR UniPathway; UPA00223; UER00717.
DR PRO; PR:Q9LXS7; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9LXS7; baseline and differential.
DR Genevisible; Q9LXS7; AT.
DR GO; GO:0005759; C:mitochondrial matrix; IBA:GO_Central.
DR GO; GO:0005777; C:peroxisome; ISS:TAIR.
DR GO; GO:0004108; F:citrate (Si)-synthase activity; IBA:GO_Central.
DR GO; GO:0005975; P:carbohydrate metabolic process; IBA:GO_Central.
DR GO; GO:0006099; P:tricarboxylic acid cycle; IBA:GO_Central.
DR Gene3D; 1.10.230.10; -; 1.
DR Gene3D; 1.10.580.10; -; 1.
DR InterPro; IPR016142; Citrate_synth-like_lrg_a-sub.
DR InterPro; IPR016143; Citrate_synth-like_sm_a-sub.
DR InterPro; IPR002020; Citrate_synthase.
DR InterPro; IPR019810; Citrate_synthase_AS.
DR InterPro; IPR024176; Citrate_synthase_bac-typ.
DR InterPro; IPR036969; Citrate_synthase_sf.
DR PANTHER; PTHR11739; PTHR11739; 1.
DR Pfam; PF00285; Citrate_synt; 1.
DR PIRSF; PIRSF001369; Citrate_synth; 1.
DR PRINTS; PR00143; CITRTSNTHASE.
DR SUPFAM; SSF48256; SSF48256; 1.
DR PROSITE; PS00480; CITRATE_SYNTHASE; 1.
PE 2: Evidence at transcript level;
KW Peroxisome; Reference proteome; Transferase; Transit peptide;
KW Tricarboxylic acid cycle.
FT TRANSIT 1..?
FT /note="Peroxisome"
FT /evidence="ECO:0000255"
FT CHAIN ?..480
FT /note="Citrate synthase 1, peroxisomal"
FT /id="PRO_0000005481"
FT ACT_SITE 321
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10117"
FT ACT_SITE 360
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10117"
FT ACT_SITE 416
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10117"
SQ SEQUENCE 480 AA; 52895 MW; 51E680C04B438C92 CRC64;
MEISERARAR LAVLNAHLTV SEPNQVLPAI EPWCTSAHIT AAPHGSLKGN LKIVDERTGN
EYQVPVSEHG TVKTVDLKKI TTGKDDKGLN LYDPGYLNTA PVRSSISYID GDEGILRYRG
YPVEELAEKS TYTEVTYLLI YGNLPSQRQL ADWEFAISQN SAVPQGVLDM IQSMPNDVHP
VGALVTAMSA LSIFYPDANP SLMGLGVYKS KQVRDKQIVR VLGQAPTIAA AAYLRKAGKP
PVQPLSNLSY SENFLYMVES MGDRSYKPNP RLARVLDILF ILQAEHEMNC STAAARHLSS
SGGDVYTAVS GGVGAIYGPL HGGAVEATIN MLSEIGTVEN IPEFIESVKN KKRRLSGFGH
RIYKNYDPRG KVVKKLADEV FSILGRDPLV EVGDALEKAA LSDEYFVKRK LYPNVDFYSG
LINRAMGIPS SFTAVSRIAG YLSHWRESLD DPDTKIMRPQ QVYTGAGIRH YETVRERTKL
