ID   CISY1_MYCTO             Reviewed;         431 AA.
AC   P9WPD4; L0T6R4; Q10530;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   25-MAY-2022, entry version 35.
DE   RecName: Full=Citrate synthase 1;
DE            EC=2.3.3.16;
GN   Name=gltA2; Synonyms=gltA; OrderedLocusNames=MT0920;
OS   Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83331;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CDC 1551 / Oshkosh;
RX   PubMed=12218036; DOI=10.1128/jb.184.19.5479-5490.2002;
RA   Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O.,
RA   Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K.,
RA   Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L.,
RA   Delcher A., Utterback T.R., Weidman J.F., Khouri H.M., Gill J., Mikula A.,
RA   Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M.;
RT   "Whole-genome comparison of Mycobacterium tuberculosis clinical and
RT   laboratory strains.";
RL   J. Bacteriol. 184:5479-5490(2002).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=acetyl-CoA + H2O + oxaloacetate = citrate + CoA + H(+);
CC         Xref=Rhea:RHEA:16845, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:16452, ChEBI:CHEBI:16947, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57288; EC=2.3.3.16; Evidence={ECO:0000255|PROSITE-
CC         ProRule:PRU10117};
CC   -!- PATHWAY: Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate
CC       from oxaloacetate: step 1/2.
CC   -!- SUBUNIT: Homohexamer. {ECO:0000250}.
CC   -!- MISCELLANEOUS: Citrate synthase is found in nearly all cells capable of
CC       oxidative metabolism. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the citrate synthase family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AE000516; AAK45166.1; -; Genomic_DNA.
DR   PIR; E70782; E70782.
DR   RefSeq; WP_003901040.1; NZ_KK341227.1.
DR   AlphaFoldDB; P9WPD4; -.
DR   SMR; P9WPD4; -.
DR   EnsemblBacteria; AAK45166; AAK45166; MT0920.
DR   GeneID; 45424859; -.
DR   KEGG; mtc:MT0920; -.
DR   PATRIC; fig|83331.31.peg.988; -.
DR   HOGENOM; CLU_025068_0_0_11; -.
DR   UniPathway; UPA00223; UER00717.
DR   Proteomes; UP000001020; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:InterPro.
DR   GO; GO:0004108; F:citrate (Si)-synthase activity; IEA:InterPro.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-UniPathway.
DR   CDD; cd06114; EcCS_like; 1.
DR   Gene3D; 1.10.230.10; -; 1.
DR   Gene3D; 1.10.580.10; -; 1.
DR   InterPro; IPR016142; Citrate_synth-like_lrg_a-sub.
DR   InterPro; IPR016143; Citrate_synth-like_sm_a-sub.
DR   InterPro; IPR002020; Citrate_synthase.
DR   InterPro; IPR019810; Citrate_synthase_AS.
DR   InterPro; IPR024176; Citrate_synthase_bac-typ.
DR   InterPro; IPR036969; Citrate_synthase_sf.
DR   InterPro; IPR010953; Citrate_synthase_typ-I.
DR   Pfam; PF00285; Citrate_synt; 1.
DR   PIRSF; PIRSF001369; Citrate_synth; 1.
DR   PRINTS; PR00143; CITRTSNTHASE.
DR   SUPFAM; SSF48256; SSF48256; 1.
DR   TIGRFAMs; TIGR01798; cit_synth_I; 1.
DR   PROSITE; PS00480; CITRATE_SYNTHASE; 1.
PE   3: Inferred from homology;
KW   Allosteric enzyme; Transferase; Tricarboxylic acid cycle.
FT   CHAIN           1..431
FT                   /note="Citrate synthase 1"
FT                   /id="PRO_0000426971"
FT   ACT_SITE        309
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10117"
FT   ACT_SITE        366
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10117"
SQ   SEQUENCE   431 AA;  47978 MW;  FA818DFFCBC5D1B9 CRC64;
     MADTDDTATL RYPGGEIDLQ IVHATEGADG IALGPLLAKT GHTTFDVGFA NTAAAKSSIT
     YIDGDAGILR YRGYPIDQLA EKSTFIEVCY LLIYGELPDT DQLAQFTGRI QRHTMLHEDL
     KRFFDGFPRN AHPMPVLSSV VNALSAYYQD ALDPMDNGQV ELSTIRLLAK LPTIAAYAYK
     KSVGQPFLYP DNSLTLVENF LRLTFGFPAE PYQADPEVVR ALDMLFILHA DHEQNCSTST
     VRLVGSSRAN LFTSISGGIN ALWGPLHGGA NQAVLEMLEG IRDSGDDVSE FVRKVKNREA
     GVKLMGFGHR VYKNYDPRAR IVKEQADKIL AKLGGDDSLL GIAKELEEAA LTDDYFIERK
     LYPNVDFYTG LIYRALGFPT RMFTVLFALG RLPGWIAHWR EMHDEGDSKI GRPRQIYTGY
     TERDYVTIDA R