CISY1_MYCTU
ID CISY1_MYCTU Reviewed; 431 AA.
AC P9WPD5; L0T6R4; Q10530;
DT 16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT 16-APR-2014, sequence version 1.
DT 03-AUG-2022, entry version 36.
DE RecName: Full=Citrate synthase 1;
DE EC=2.3.3.16;
GN Name=gltA2; Synonyms=gltA; OrderedLocusNames=Rv0896; ORFNames=MTCY31.24;
OS Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC Mycobacterium; Mycobacterium tuberculosis complex.
OX NCBI_TaxID=83332;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=9634230; DOI=10.1038/31159;
RA Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA Barrell B.G.;
RT "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT genome sequence.";
RL Nature 393:537-544(1998).
RN [2]
RP PUPYLATION AT LYS-328, AND IDENTIFICATION BY MASS SPECTROMETRY.
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=20066036; DOI=10.1371/journal.pone.0008589;
RA Festa R.A., McAllister F., Pearce M.J., Mintseris J., Burns K.E.,
RA Gygi S.P., Darwin K.H.;
RT "Prokaryotic ubiquitin-like protein (Pup) proteome of Mycobacterium
RT tuberculosis.";
RL PLoS ONE 5:E8589-E8589(2010).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=21969609; DOI=10.1074/mcp.m111.011627;
RA Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K.,
RA Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R.,
RA Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M.,
RA Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.;
RT "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution
RT mass spectrometry.";
RL Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=acetyl-CoA + H2O + oxaloacetate = citrate + CoA + H(+);
CC Xref=Rhea:RHEA:16845, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:16452, ChEBI:CHEBI:16947, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:57288; EC=2.3.3.16; Evidence={ECO:0000255|PROSITE-
CC ProRule:PRU10117};
CC -!- PATHWAY: Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate
CC from oxaloacetate: step 1/2.
CC -!- SUBUNIT: Homohexamer. {ECO:0000250}.
CC -!- MISCELLANEOUS: Citrate synthase is found in nearly all cells capable of
CC oxidative metabolism. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the citrate synthase family. {ECO:0000305}.
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DR EMBL; AL123456; CCP43644.1; -; Genomic_DNA.
DR PIR; E70782; E70782.
DR RefSeq; NP_215411.1; NC_000962.3.
DR RefSeq; WP_003901040.1; NZ_NVQJ01000001.1.
DR PDB; 4TVM; X-ray; 2.60 A; A=2-431.
DR PDBsum; 4TVM; -.
DR AlphaFoldDB; P9WPD5; -.
DR SMR; P9WPD5; -.
DR STRING; 83332.Rv0896; -.
DR PaxDb; P9WPD5; -.
DR DNASU; 885208; -.
DR GeneID; 45424859; -.
DR GeneID; 885208; -.
DR KEGG; mtu:Rv0896; -.
DR TubercuList; Rv0896; -.
DR eggNOG; COG0372; Bacteria.
DR OMA; TVGWCAQ; -.
DR PhylomeDB; P9WPD5; -.
DR BioCyc; MetaCyc:G185E-5050-MON; -.
DR BRENDA; 2.3.3.16; 3445.
DR UniPathway; UPA00223; UER00717.
DR Proteomes; UP000001584; Chromosome.
DR GO; GO:0005829; C:cytosol; HDA:MTBBASE.
DR GO; GO:0005886; C:plasma membrane; HDA:MTBBASE.
DR GO; GO:0004108; F:citrate (Si)-synthase activity; IEA:InterPro.
DR GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-UniPathway.
DR CDD; cd06114; EcCS_like; 1.
DR Gene3D; 1.10.230.10; -; 1.
DR Gene3D; 1.10.580.10; -; 1.
DR InterPro; IPR016142; Citrate_synth-like_lrg_a-sub.
DR InterPro; IPR016143; Citrate_synth-like_sm_a-sub.
DR InterPro; IPR002020; Citrate_synthase.
DR InterPro; IPR019810; Citrate_synthase_AS.
DR InterPro; IPR024176; Citrate_synthase_bac-typ.
DR InterPro; IPR036969; Citrate_synthase_sf.
DR InterPro; IPR010953; Citrate_synthase_typ-I.
DR Pfam; PF00285; Citrate_synt; 1.
DR PIRSF; PIRSF001369; Citrate_synth; 1.
DR PRINTS; PR00143; CITRTSNTHASE.
DR SUPFAM; SSF48256; SSF48256; 1.
DR TIGRFAMs; TIGR01798; cit_synth_I; 1.
DR PROSITE; PS00480; CITRATE_SYNTHASE; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Allosteric enzyme; Isopeptide bond; Reference proteome;
KW Transferase; Tricarboxylic acid cycle; Ubl conjugation.
FT CHAIN 1..431
FT /note="Citrate synthase 1"
FT /id="PRO_0000169948"
FT ACT_SITE 309
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10117"
FT ACT_SITE 366
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10117"
FT CROSSLNK 328
FT /note="Isoglutamyl lysine isopeptide (Lys-Gln) (interchain
FT with Q-Cter in protein Pup)"
FT /evidence="ECO:0000269|PubMed:20066036"
FT STRAND 8..10
FT /evidence="ECO:0007829|PDB:4TVM"
FT STRAND 17..19
FT /evidence="ECO:0007829|PDB:4TVM"
FT STRAND 25..27
FT /evidence="ECO:0007829|PDB:4TVM"
FT TURN 37..39
FT /evidence="ECO:0007829|PDB:4TVM"
FT HELIX 47..49
FT /evidence="ECO:0007829|PDB:4TVM"
FT STRAND 59..63
FT /evidence="ECO:0007829|PDB:4TVM"
FT TURN 64..67
FT /evidence="ECO:0007829|PDB:4TVM"
FT STRAND 68..71
FT /evidence="ECO:0007829|PDB:4TVM"
FT HELIX 76..82
FT /evidence="ECO:0007829|PDB:4TVM"
FT HELIX 85..94
FT /evidence="ECO:0007829|PDB:4TVM"
FT HELIX 100..111
FT /evidence="ECO:0007829|PDB:4TVM"
FT HELIX 118..122
FT /evidence="ECO:0007829|PDB:4TVM"
FT HELIX 123..126
FT /evidence="ECO:0007829|PDB:4TVM"
FT HELIX 133..147
FT /evidence="ECO:0007829|PDB:4TVM"
FT HELIX 149..151
FT /evidence="ECO:0007829|PDB:4TVM"
FT STRAND 154..156
FT /evidence="ECO:0007829|PDB:4TVM"
FT HELIX 159..182
FT /evidence="ECO:0007829|PDB:4TVM"
FT HELIX 196..205
FT /evidence="ECO:0007829|PDB:4TVM"
FT HELIX 216..229
FT /evidence="ECO:0007829|PDB:4TVM"
FT HELIX 236..246
FT /evidence="ECO:0007829|PDB:4TVM"
FT HELIX 251..262
FT /evidence="ECO:0007829|PDB:4TVM"
FT TURN 265..269
FT /evidence="ECO:0007829|PDB:4TVM"
FT HELIX 270..281
FT /evidence="ECO:0007829|PDB:4TVM"
FT HELIX 317..329
FT /evidence="ECO:0007829|PDB:4TVM"
FT HELIX 339..348
FT /evidence="ECO:0007829|PDB:4TVM"
FT HELIX 367..375
FT /evidence="ECO:0007829|PDB:4TVM"
FT HELIX 380..382
FT /evidence="ECO:0007829|PDB:4TVM"
FT HELIX 383..403
FT /evidence="ECO:0007829|PDB:4TVM"
SQ SEQUENCE 431 AA; 47978 MW; FA818DFFCBC5D1B9 CRC64;
MADTDDTATL RYPGGEIDLQ IVHATEGADG IALGPLLAKT GHTTFDVGFA NTAAAKSSIT
YIDGDAGILR YRGYPIDQLA EKSTFIEVCY LLIYGELPDT DQLAQFTGRI QRHTMLHEDL
KRFFDGFPRN AHPMPVLSSV VNALSAYYQD ALDPMDNGQV ELSTIRLLAK LPTIAAYAYK
KSVGQPFLYP DNSLTLVENF LRLTFGFPAE PYQADPEVVR ALDMLFILHA DHEQNCSTST
VRLVGSSRAN LFTSISGGIN ALWGPLHGGA NQAVLEMLEG IRDSGDDVSE FVRKVKNREA
GVKLMGFGHR VYKNYDPRAR IVKEQADKIL AKLGGDDSLL GIAKELEEAA LTDDYFIERK
LYPNVDFYTG LIYRALGFPT RMFTVLFALG RLPGWIAHWR EMHDEGDSKI GRPRQIYTGY
TERDYVTIDA R
