ID   CISY2_RHITR             Reviewed;         429 AA.
AC   P51038;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   03-AUG-2022, entry version 90.
DE   RecName: Full=Citrate synthase, plasmid;
DE            EC=2.3.3.16;
GN   Name=pcsA;
OS   Rhizobium tropici.
OG   Plasmid sym.
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Rhizobium/Agrobacterium group; Rhizobium.
OX   NCBI_TaxID=398;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=CFN 299;
RX   PubMed=8170393; DOI=10.1111/j.1365-2958.1994.tb00311.x;
RA   Pardo M.A., Lagunez J., Miranda J., Martinez E.;
RT   "Nodulating ability of Rhizobium tropici is conditioned by a plasmid-
RT   encoded citrate synthase.";
RL   Mol. Microbiol. 11:315-321(1994).
CC   -!- FUNCTION: The exact function of the plasmid-encoded citrate synthase is
CC       not clear, it could help nodulation by allowing the bacteria to use
CC       citrate as a chelator of iron and calcium.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=acetyl-CoA + H2O + oxaloacetate = citrate + CoA + H(+);
CC         Xref=Rhea:RHEA:16845, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:16452, ChEBI:CHEBI:16947, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57288; EC=2.3.3.16; Evidence={ECO:0000255|PROSITE-
CC         ProRule:PRU10117};
CC   -!- PATHWAY: Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate
CC       from oxaloacetate: step 1/2.
CC   -!- SIMILARITY: Belongs to the citrate synthase family. {ECO:0000305}.
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DR   EMBL; Z34516; CAA84274.1; -; Genomic_DNA.
DR   PIR; S41527; S41527.
DR   AlphaFoldDB; P51038; -.
DR   SMR; P51038; -.
DR   PRIDE; P51038; -.
DR   UniPathway; UPA00223; UER00717.
DR   GO; GO:0005737; C:cytoplasm; IEA:InterPro.
DR   GO; GO:0004108; F:citrate (Si)-synthase activity; IEA:InterPro.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-UniPathway.
DR   CDD; cd06114; EcCS_like; 1.
DR   Gene3D; 1.10.230.10; -; 1.
DR   Gene3D; 1.10.580.10; -; 1.
DR   InterPro; IPR016142; Citrate_synth-like_lrg_a-sub.
DR   InterPro; IPR016143; Citrate_synth-like_sm_a-sub.
DR   InterPro; IPR002020; Citrate_synthase.
DR   InterPro; IPR019810; Citrate_synthase_AS.
DR   InterPro; IPR024176; Citrate_synthase_bac-typ.
DR   InterPro; IPR036969; Citrate_synthase_sf.
DR   InterPro; IPR010953; Citrate_synthase_typ-I.
DR   Pfam; PF00285; Citrate_synt; 1.
DR   PIRSF; PIRSF001369; Citrate_synth; 1.
DR   PRINTS; PR00143; CITRTSNTHASE.
DR   SUPFAM; SSF48256; SSF48256; 1.
DR   TIGRFAMs; TIGR01798; cit_synth_I; 1.
DR   PROSITE; PS00480; CITRATE_SYNTHASE; 1.
PE   3: Inferred from homology;
KW   Plasmid; Transferase; Tricarboxylic acid cycle.
FT   CHAIN           1..429
FT                   /note="Citrate synthase, plasmid"
FT                   /id="PRO_0000169955"
FT   ACT_SITE        306
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10117"
FT   ACT_SITE        364
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10117"
SQ   SEQUENCE   429 AA;  48105 MW;  C2E4560E4FE03485 CRC64;
     MDNNNACVLV DGHSAELKLR SSTIGPNVLG IGSLYEQTKM FTYDPGFTST ASCESSITFI
     DGDEGVLLHR GYPIEQLAEH GDFLEVCYLL LYGELPTAAQ KKDFDYRVVH HTMVHEQMSR
     FFTGFRRDAH PMAVMCGCVG ALSAFYHDST DITDPHQRMV ASLRMIAKMP TLAAMAYKYH
     IGQPFVYPKN DLDYASNFLR MCFAVPCEEY VVNPVLARAM DRIFILHADH EQNASTSTVR
     LAGSSGANPF ACIAAGIACL WGPAHGGANE RALNMLTEIG TVDRIPEYIA RAKDKNDPFR
     LMGFGHRVYK NYDPRAKIMQ KTAHEVLGEL GIKDDPLLDI AIELERIALT DDYFIEKKLY
     PNVDFYSGIT LKALGFPTTM FTVLFALART VGWIAQWNEM IEDPDQRIGR PRQLYTGAPL
     REYVPLSKR