CISY3_ARATH
ID CISY3_ARATH Reviewed; 509 AA.
AC Q9SJH7; Q56YQ7;
DT 30-AUG-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 136.
DE RecName: Full=Citrate synthase 3, peroxisomal;
DE EC=2.3.3.16;
DE Flags: Precursor;
GN Name=CSY3; OrderedLocusNames=At2g42790; ORFNames=F7D19.21;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 369-509.
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND DEVELOPMENTAL
RP STAGE.
RX PubMed=15923350; DOI=10.1105/tpc.105.031856;
RA Pracharoenwattana I., Cornah J.E., Smith S.M.;
RT "Arabidopsis peroxisomal citrate synthase is required for fatty acid
RT respiration and seed germination.";
RL Plant Cell 17:2037-2048(2005).
CC -!- FUNCTION: Peroxisomal citrate synthase required for the fatty acid
CC respiration in seedlings, citrate being exported from peroxisomes into
CC mitochondria during respiration of triacylglycerol (TAG). Indeed,
CC complete respiration requires the transfer of carbon in the form of
CC citrate from the peroxisome to the mitochondria.
CC {ECO:0000269|PubMed:15923350}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=acetyl-CoA + H2O + oxaloacetate = citrate + CoA + H(+);
CC Xref=Rhea:RHEA:16845, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:16452, ChEBI:CHEBI:16947, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:57288; EC=2.3.3.16; Evidence={ECO:0000255|PROSITE-
CC ProRule:PRU10117};
CC -!- PATHWAY: Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate
CC from oxaloacetate: step 1/2.
CC -!- SUBCELLULAR LOCATION: Peroxisome {ECO:0000269|PubMed:15923350}.
CC -!- TISSUE SPECIFICITY: Widely expressed. Expressed throughout the shoot.
CC Expressed in flower, silique, stem, cauline leaf, young leaf, mature
CC leaf and senescent leaf. {ECO:0000269|PubMed:15923350}.
CC -!- DEVELOPMENTAL STAGE: Expressed throughout seedling growth.
CC {ECO:0000269|PubMed:15923350}.
CC -!- MISCELLANEOUS: Citrate synthase is found in nearly all cells capable of
CC oxidative metabolism.
CC -!- SIMILARITY: Belongs to the citrate synthase family. {ECO:0000305}.
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DR EMBL; AC006931; AAD21729.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC10169.1; -; Genomic_DNA.
DR EMBL; AY048214; AAK82477.1; -; mRNA.
DR EMBL; AY124841; AAM70550.1; -; mRNA.
DR EMBL; AK221264; BAD93930.1; -; mRNA.
DR PIR; C84858; C84858.
DR RefSeq; NP_181807.1; NM_129840.5.
DR AlphaFoldDB; Q9SJH7; -.
DR SMR; Q9SJH7; -.
DR BioGRID; 4216; 33.
DR IntAct; Q9SJH7; 17.
DR STRING; 3702.AT2G42790.1; -.
DR PaxDb; Q9SJH7; -.
DR PRIDE; Q9SJH7; -.
DR ProteomicsDB; 246864; -.
DR EnsemblPlants; AT2G42790.1; AT2G42790.1; AT2G42790.
DR GeneID; 818879; -.
DR Gramene; AT2G42790.1; AT2G42790.1; AT2G42790.
DR KEGG; ath:AT2G42790; -.
DR Araport; AT2G42790; -.
DR TAIR; locus:2052379; AT2G42790.
DR eggNOG; KOG2617; Eukaryota.
DR HOGENOM; CLU_025068_0_1_1; -.
DR InParanoid; Q9SJH7; -.
DR OMA; QGFMAHW; -.
DR OrthoDB; 691172at2759; -.
DR PhylomeDB; Q9SJH7; -.
DR BioCyc; ARA:AT2G42790-MON; -.
DR UniPathway; UPA00223; UER00717.
DR PRO; PR:Q9SJH7; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q9SJH7; baseline and differential.
DR Genevisible; Q9SJH7; AT.
DR GO; GO:0005829; C:cytosol; HDA:TAIR.
DR GO; GO:0005759; C:mitochondrial matrix; IBA:GO_Central.
DR GO; GO:0005777; C:peroxisome; IDA:TAIR.
DR GO; GO:0004108; F:citrate (Si)-synthase activity; IGI:TAIR.
DR GO; GO:0005975; P:carbohydrate metabolic process; IBA:GO_Central.
DR GO; GO:0006635; P:fatty acid beta-oxidation; IGI:TAIR.
DR GO; GO:0006099; P:tricarboxylic acid cycle; IBA:GO_Central.
DR Gene3D; 1.10.230.10; -; 1.
DR Gene3D; 1.10.580.10; -; 1.
DR InterPro; IPR016142; Citrate_synth-like_lrg_a-sub.
DR InterPro; IPR016143; Citrate_synth-like_sm_a-sub.
DR InterPro; IPR002020; Citrate_synthase.
DR InterPro; IPR019810; Citrate_synthase_AS.
DR InterPro; IPR036969; Citrate_synthase_sf.
DR PANTHER; PTHR11739; PTHR11739; 1.
DR Pfam; PF00285; Citrate_synt; 1.
DR PRINTS; PR00143; CITRTSNTHASE.
DR SUPFAM; SSF48256; SSF48256; 1.
DR PROSITE; PS00480; CITRATE_SYNTHASE; 1.
PE 2: Evidence at transcript level;
KW Peroxisome; Reference proteome; Transferase; Transit peptide;
KW Tricarboxylic acid cycle.
FT TRANSIT 1..?
FT /note="Peroxisome"
FT /evidence="ECO:0000255"
FT CHAIN ?..509
FT /note="Citrate synthase 3, peroxisomal"
FT /id="PRO_0000005483"
FT REGION 485..509
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 491..509
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 319
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10117"
FT ACT_SITE 358
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10117"
FT ACT_SITE 414
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10117"
FT CONFLICT 430
FT /note="F -> L (in Ref. 4; BAD93930)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 509 AA; 56175 MW; 5ADF4A63F1DDFE38 CRC64;
MEISERVRAR LAVLSGHLSE GKQDSPAIER WCTSADTSVA PLGSLKGTLT IVDERTGKNY
KVPVSDDGTV KAVDFKKIVT GKEDKGLKLY DPGYLNTAPV RSSISYIDGD EGILRYRGYP
IEEMAENSTF LEVAYLLMYG NLPSESQLSD WEFAVSQHSA VPQGVLDIIQ SMPHDAHPMG
VLVSAMSALS IFHPDANPAL RGQDIYDSKQ VRDKQIIRII GKAPTIAAAA YLRMAGRPPV
LPSGNLPYAD NFLYMLDSLG NRSYKPNPRL ARVLDILFIL HAEHEMNCST AAARHLASSG
VDVYTAVAGA VGALYGPLHG GANEAVLKML SEIGTVENIP EFIEGVKNRK RKMSGFGHRV
YKNYDPRAKV IKNLADEVFS IVGKDPLIEV AVALEKAALS DDYFVKRKLY PNVDFYSGLI
YRAMGFPPEF FTVLFAIPRM AGYLSHWKES LDDPDTKIMR PQQVYTGVWL RHYTPVRERI
VTDDSKESDK LGQVATSNAS RRRLAGSSV
