CISYC_DICDI
ID CISYC_DICDI Reviewed; 512 AA.
AC Q86AV6; Q550V8;
DT 08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 03-AUG-2022, entry version 104.
DE RecName: Full=Citrate synthase;
DE EC=2.3.3.16;
GN Name=gltA; ORFNames=DDB_G0276965;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=12097910; DOI=10.1038/nature00847;
RA Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T.,
RA Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R.,
RA Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A.,
RA Noegel A.A.;
RT "Sequence and analysis of chromosome 2 of Dictyostelium discoideum.";
RL Nature 418:79-85(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [3]
RP SUBCELLULAR LOCATION.
RC STRAIN=AX2;
RX PubMed=15225305; DOI=10.1111/j.1365-2958.2004.04109.x;
RA Huang Y.-C., Chen Y.-H., Lo S.-R., Liu C.-I., Wang C.-W., Chang W.-T.;
RT "Disruption of the peroxisomal citrate synthase CshA affects cell growth
RT and multicellular development in Dictyostelium discoideum.";
RL Mol. Microbiol. 53:81-91(2004).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=acetyl-CoA + H2O + oxaloacetate = citrate + CoA + H(+);
CC Xref=Rhea:RHEA:16845, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:16452, ChEBI:CHEBI:16947, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:57288; EC=2.3.3.16; Evidence={ECO:0000255|PROSITE-
CC ProRule:PRU10117};
CC -!- PATHWAY: Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate
CC from oxaloacetate: step 1/2.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:15225305}.
CC -!- SIMILARITY: Belongs to the citrate synthase family. {ECO:0000305}.
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DR EMBL; AAFI02000019; EAL68984.1; -; Genomic_DNA.
DR RefSeq; XP_642824.1; XM_637732.1.
DR AlphaFoldDB; Q86AV6; -.
DR SMR; Q86AV6; -.
DR STRING; 44689.DDB0214944; -.
DR PaxDb; Q86AV6; -.
DR PRIDE; Q86AV6; -.
DR EnsemblProtists; EAL68984; EAL68984; DDB_G0276965.
DR GeneID; 8620687; -.
DR KEGG; ddi:DDB_G0276965; -.
DR dictyBase; DDB_G0276965; gltA.
DR eggNOG; KOG2617; Eukaryota.
DR HOGENOM; CLU_025068_0_1_1; -.
DR InParanoid; Q86AV6; -.
DR OMA; DRQLYPN; -.
DR PhylomeDB; Q86AV6; -.
DR UniPathway; UPA00223; UER00717.
DR PRO; PR:Q86AV6; -.
DR Proteomes; UP000002195; Chromosome 2.
DR GO; GO:0005829; C:cytosol; NAS:dictyBase.
DR GO; GO:0005759; C:mitochondrial matrix; IBA:GO_Central.
DR GO; GO:0004108; F:citrate (Si)-synthase activity; ISS:dictyBase.
DR GO; GO:0005975; P:carbohydrate metabolic process; IBA:GO_Central.
DR GO; GO:0006097; P:glyoxylate cycle; ISS:dictyBase.
DR GO; GO:0006099; P:tricarboxylic acid cycle; IBA:GO_Central.
DR Gene3D; 1.10.230.10; -; 1.
DR Gene3D; 1.10.580.10; -; 1.
DR InterPro; IPR016142; Citrate_synth-like_lrg_a-sub.
DR InterPro; IPR016143; Citrate_synth-like_sm_a-sub.
DR InterPro; IPR002020; Citrate_synthase.
DR InterPro; IPR019810; Citrate_synthase_AS.
DR InterPro; IPR036969; Citrate_synthase_sf.
DR InterPro; IPR010953; Citrate_synthase_typ-I.
DR PANTHER; PTHR11739; PTHR11739; 1.
DR Pfam; PF00285; Citrate_synt; 1.
DR PRINTS; PR00143; CITRTSNTHASE.
DR SUPFAM; SSF48256; SSF48256; 1.
DR TIGRFAMs; TIGR01798; cit_synth_I; 1.
DR PROSITE; PS00480; CITRATE_SYNTHASE; 1.
PE 3: Inferred from homology;
KW Cytoplasm; Reference proteome; Transferase; Tricarboxylic acid cycle.
FT CHAIN 1..512
FT /note="Citrate synthase"
FT /id="PRO_0000327838"
FT REGION 483..512
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 487..512
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 288
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10117"
FT ACT_SITE 327
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10117"
FT ACT_SITE 383
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10117"
SQ SEQUENCE 512 AA; 58291 MW; F10C4D0F7992649D CRC64;
MSSNSQEDNN KTTEKKNTLT VIDNRTGKSY EIPINHETVK SIDFRAIKEQ SIDFGTMIYD
PGYYNTAVCK SQITYIDGDR GILEYRGYPI EQLAEKSSFL EVSYLLIYGD LPSKEQSNLW
NTKIMNHTFI HENLISMMKS FRYDAHPMGM LISSLSAMST FYPEANPALA GVDIYKNKQL
MNKQIFRILG KLPTIAACAY RHRIGRPYND PSNTLSYTEN FLYMLDRLSE SNYKPHPVLT
RALDKLFIIH ADHELNCSTA TMRQIASTLV DPYTACAGSA GALYGPLHGG ANEAVLRMLE
AIGTIENIPK FIEQVKQKKQ RLMGFGHRVY KSYDPRAKIL KTVTMEIFAL LGKNPLMQIA
TELERLALSD SYFIERQLYP NVDFYSGIIY KSMGFPTDMF PVLFSIPRAA GWLAHWVEEL
ADPELRIFRP RQIYMGRRNM NYVPMDARQV QQHNSGEKLS SFSSGFDRRR DVSEELFNFE
DGAIPKTATG SKSQLSASIE QSFGEKISPQ SH
