CISYP_DICDI
ID CISYP_DICDI Reviewed; 492 AA.
AC Q8MQU6; Q55FD7;
DT 08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 25-MAY-2022, entry version 110.
DE RecName: Full=Citrate synthase, peroxisomal;
DE EC=2.3.3.16;
DE Flags: Precursor;
GN Name=cshA; ORFNames=DDB_G0267426;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION, AND
RP DEVELOPMENTAL STAGE.
RC STRAIN=AX2;
RX PubMed=15225305; DOI=10.1111/j.1365-2958.2004.04109.x;
RA Huang Y.-C., Chen Y.-H., Lo S.-R., Liu C.-I., Wang C.-W., Chang W.-T.;
RT "Disruption of the peroxisomal citrate synthase CshA affects cell growth
RT and multicellular development in Dictyostelium discoideum.";
RL Mol. Microbiol. 53:81-91(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
CC -!- FUNCTION: Peroxisomal protein involved in the cellular biosynthesis of
CC citrate, and required primarily for cell growth and modulation of
CC multicellular development. {ECO:0000269|PubMed:15225305}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=acetyl-CoA + H2O + oxaloacetate = citrate + CoA + H(+);
CC Xref=Rhea:RHEA:16845, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:16452, ChEBI:CHEBI:16947, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:57288; EC=2.3.3.16; Evidence={ECO:0000255|PROSITE-
CC ProRule:PRU10117};
CC -!- PATHWAY: Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate
CC from oxaloacetate: step 1/2.
CC -!- SUBCELLULAR LOCATION: Peroxisome {ECO:0000269|PubMed:15225305}.
CC -!- DEVELOPMENTAL STAGE: Maximally expressed during vegetative growth and
CC gradually decreased through subsequent developmental stages.
CC {ECO:0000269|PubMed:15225305}.
CC -!- SIMILARITY: Belongs to the citrate synthase family. {ECO:0000305}.
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DR EMBL; AY124377; AAM92490.1; -; mRNA.
DR EMBL; AAFI02000003; EAL73165.1; -; Genomic_DNA.
DR RefSeq; XP_647596.1; XM_642504.1.
DR AlphaFoldDB; Q8MQU6; -.
DR SMR; Q8MQU6; -.
DR BioGRID; 1241411; 1.
DR STRING; 44689.DDB0191110; -.
DR PaxDb; Q8MQU6; -.
DR EnsemblProtists; EAL73165; EAL73165; DDB_G0267426.
DR GeneID; 8616408; -.
DR KEGG; ddi:DDB_G0267426; -.
DR dictyBase; DDB_G0267426; cshA.
DR eggNOG; KOG2617; Eukaryota.
DR HOGENOM; CLU_025068_0_1_1; -.
DR InParanoid; Q8MQU6; -.
DR OMA; HADHEMN; -.
DR PhylomeDB; Q8MQU6; -.
DR UniPathway; UPA00223; UER00717.
DR PRO; PR:Q8MQU6; -.
DR Proteomes; UP000002195; Chromosome 1.
DR GO; GO:0005759; C:mitochondrial matrix; IBA:GO_Central.
DR GO; GO:0005777; C:peroxisome; IDA:dictyBase.
DR GO; GO:0004108; F:citrate (Si)-synthase activity; IDA:dictyBase.
DR GO; GO:0019954; P:asexual reproduction; IMP:dictyBase.
DR GO; GO:0005975; P:carbohydrate metabolic process; IBA:GO_Central.
DR GO; GO:0006897; P:endocytosis; IMP:dictyBase.
DR GO; GO:0006097; P:glyoxylate cycle; ISS:dictyBase.
DR GO; GO:0006909; P:phagocytosis; IMP:dictyBase.
DR GO; GO:0006907; P:pinocytosis; IMP:dictyBase.
DR GO; GO:0050766; P:positive regulation of phagocytosis; IMP:dictyBase.
DR GO; GO:0031288; P:sorocarp morphogenesis; IMP:dictyBase.
DR GO; GO:0006099; P:tricarboxylic acid cycle; IBA:GO_Central.
DR Gene3D; 1.10.230.10; -; 1.
DR Gene3D; 1.10.580.10; -; 1.
DR InterPro; IPR016142; Citrate_synth-like_lrg_a-sub.
DR InterPro; IPR016143; Citrate_synth-like_sm_a-sub.
DR InterPro; IPR002020; Citrate_synthase.
DR InterPro; IPR019810; Citrate_synthase_AS.
DR InterPro; IPR036969; Citrate_synthase_sf.
DR InterPro; IPR010953; Citrate_synthase_typ-I.
DR PANTHER; PTHR11739; PTHR11739; 1.
DR Pfam; PF00285; Citrate_synt; 1.
DR PRINTS; PR00143; CITRTSNTHASE.
DR SUPFAM; SSF48256; SSF48256; 1.
DR TIGRFAMs; TIGR01798; cit_synth_I; 1.
DR PROSITE; PS00480; CITRATE_SYNTHASE; 1.
PE 2: Evidence at transcript level;
KW Peroxisome; Reference proteome; Transferase; Transit peptide;
KW Tricarboxylic acid cycle.
FT TRANSIT 1..?
FT /note="Peroxisome"
FT /evidence="ECO:0000255"
FT CHAIN ?..492
FT /note="Citrate synthase, peroxisomal"
FT /id="PRO_0000327837"
FT REGION 469..492
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 473..492
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 307
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10117"
FT ACT_SITE 346
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10117"
FT ACT_SITE 402
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10117"
SQ SEQUENCE 492 AA; 55749 MW; 2008B6988B19A597 CRC64;
MAHIDRINIL ANHLSKEEEE ENELLTSPVS ADKKKETVTV TDNRNGKSYD FKIKNDTINA
LNFKELQLAK GDGGLMIYDP GFQNTAVVTS YITYIDGDKG ILRYRGYPIE ELAERSNFLE
VAYLLINGNL PNKSQLDGWS NKIMTHTFLH ENLVGLMKTF RYDAHPMGML ISTVAALGTF
YPEANPALAG GDIFKSENVR NKQIYRIVGK LPTIAACAWR HRIGRPYNTP VNHLGYTENF
LYMLDKLSEP DYKPNPVLCR ALEILFILHA DHELNCSTAA MRHISSSGTD PYTSVAGAAG
ALYGPSHGGA NEAVLDMLIH IGSKENIPQF ISDVKSKKKK LMGFGHRIYK NYDPRAKIIR
RVAYEVFESL GKEPLIEVAT ELEKQALEDE YFVSRKLYPN VDFYSGLIYK AMGFPTDMFP
VLFTIPRAVG WLAHWVEHLE DPETKIYRPR QVYKGEWFRN YVPIDGRPPA KVRSQDSYSS
ATTKRYSKVT SH
