ID   CISY_ACIAN              Reviewed;         424 AA.
AC   P20902;
DT   01-FEB-1991, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 2.
DT   25-MAY-2022, entry version 97.
DE   RecName: Full=Citrate synthase;
DE            EC=2.3.3.16;
GN   Name=gltA;
OS   Acinetobacter calcoaceticus subsp. anitratus.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Moraxellales; Moraxellaceae;
OC   Acinetobacter; Acinetobacter calcoaceticus/baumannii complex.
OX   NCBI_TaxID=107673;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3442626; DOI=10.1139/o87-121;
RA   Donald L.J., Duckworth H.W.;
RT   "Expression and base sequence of the citrate synthase gene of Acinetobacter
RT   anitratum.";
RL   Biochem. Cell Biol. 65:930-938(1987).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=acetyl-CoA + H2O + oxaloacetate = citrate + CoA + H(+);
CC         Xref=Rhea:RHEA:16845, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:16452, ChEBI:CHEBI:16947, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57288; EC=2.3.3.16; Evidence={ECO:0000255|PROSITE-
CC         ProRule:PRU10117};
CC   -!- PATHWAY: Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate
CC       from oxaloacetate: step 1/2.
CC   -!- SUBUNIT: Homohexamer.
CC   -!- MISCELLANEOUS: Citrate synthase is found in nearly all cells capable of
CC       oxidative metabolism.
CC   -!- SIMILARITY: Belongs to the citrate synthase family. {ECO:0000305}.
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DR   EMBL; M33037; AAA21893.1; -; Genomic_DNA.
DR   PIR; I39506; I39506.
DR   AlphaFoldDB; P20902; -.
DR   SMR; P20902; -.
DR   SABIO-RK; P20902; -.
DR   UniPathway; UPA00223; UER00717.
DR   GO; GO:0005737; C:cytoplasm; IEA:InterPro.
DR   GO; GO:0004108; F:citrate (Si)-synthase activity; IEA:InterPro.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-UniPathway.
DR   CDD; cd06114; EcCS_like; 1.
DR   Gene3D; 1.10.230.10; -; 1.
DR   Gene3D; 1.10.580.10; -; 1.
DR   InterPro; IPR016142; Citrate_synth-like_lrg_a-sub.
DR   InterPro; IPR016143; Citrate_synth-like_sm_a-sub.
DR   InterPro; IPR002020; Citrate_synthase.
DR   InterPro; IPR019810; Citrate_synthase_AS.
DR   InterPro; IPR024176; Citrate_synthase_bac-typ.
DR   InterPro; IPR036969; Citrate_synthase_sf.
DR   InterPro; IPR010953; Citrate_synthase_typ-I.
DR   Pfam; PF00285; Citrate_synt; 1.
DR   PIRSF; PIRSF001369; Citrate_synth; 1.
DR   PRINTS; PR00143; CITRTSNTHASE.
DR   SUPFAM; SSF48256; SSF48256; 1.
DR   TIGRFAMs; TIGR01798; cit_synth_I; 1.
DR   PROSITE; PS00480; CITRATE_SYNTHASE; 1.
PE   3: Inferred from homology;
KW   Allosteric enzyme; Transferase; Tricarboxylic acid cycle.
FT   INIT_MET        1
FT                   /note="Removed"
FT   CHAIN           2..424
FT                   /note="Citrate synthase"
FT                   /id="PRO_0000169924"
FT   ACT_SITE        307
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10117"
FT   ACT_SITE        364
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10117"
SQ   SEQUENCE   424 AA;  47461 MW;  9EF5DA81E9FEB41E CRC64;
     MSEATGKKAV LHLDGKEIEL PIYSGTLGPD VIDVKDVLAS GHFTFDPGFM ATASCESKIT
     FIDGDKGILL HRGYPIDQLA TQADYLETCY LLLNGELPTA EQKVEFDAKV RAHTMVHDQV
     SRFFNGFRRD AHPMAIMVGV VGALSAFYHN NLDIEDINHR EITAIRLIAK IPTLAAWSYK
     YTVGQPFIYP RNDLNYAENF LHMMFATPAD RDYKVNPVLA RAMDRIFTLH ADHEQNASTS
     TVRLAGSTGA NPYACISAGI SALWGPAHGG ANEAVLKMLD EIGSVENVAE FMEKVKRKEV
     KLMGFGHRVY KNFDPRAKVM KQTCDEVLEA LGINDPQLAL AMELERIALN DPYFVERKLY
     PNVDFYSGII LKAIGIPTEM FTVIFALART VGWISHWLEM HSGPYKIGRP RQLYTGEVQR
     DIKR