ID   CISY_BARBA              Reviewed;         321 AA.
AC   P51031; Q59184;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 2.
DT   25-MAY-2022, entry version 85.
DE   RecName: Full=Citrate synthase;
DE            EC=2.3.3.16;
DE   Flags: Fragment;
GN   Name=gltA;
OS   Bartonella bacilliformis.
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Bartonellaceae; Bartonella.
OX   NCBI_TaxID=774;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-303.
RC   STRAIN=LA6.3;
RX   PubMed=8863415; DOI=10.1099/00207713-46-4-891;
RA   Birtles R.J., Raoult D.;
RT   "Comparison of partial citrate synthase gene (gltA) sequences for
RT   phylogenetic analysis of Bartonella species.";
RL   Int. J. Syst. Bacteriol. 46:891-897(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 210-321.
RC   STRAIN=ATCC 35686 / KC584 / NCTC 12139;
RA   Jones D.C., Regnery R., Bowen M.;
RL   Submitted (MAY-1995) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=acetyl-CoA + H2O + oxaloacetate = citrate + CoA + H(+);
CC         Xref=Rhea:RHEA:16845, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:16452, ChEBI:CHEBI:16947, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57288; EC=2.3.3.16; Evidence={ECO:0000255|PROSITE-
CC         ProRule:PRU10117};
CC   -!- PATHWAY: Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate
CC       from oxaloacetate: step 1/2.
CC   -!- MISCELLANEOUS: Citrate synthase is found in nearly all cells capable of
CC       oxidative metabolism.
CC   -!- SIMILARITY: Belongs to the citrate synthase family. {ECO:0000305}.
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DR   EMBL; Z70021; CAA93843.1; -; Genomic_DNA.
DR   EMBL; U28076; AAA74976.1; -; Genomic_DNA.
DR   AlphaFoldDB; P51031; -.
DR   SMR; P51031; -.
DR   UniPathway; UPA00223; UER00717.
DR   GO; GO:0005737; C:cytoplasm; IEA:InterPro.
DR   GO; GO:0004108; F:citrate (Si)-synthase activity; IEA:InterPro.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-UniPathway.
DR   Gene3D; 1.10.230.10; -; 1.
DR   Gene3D; 1.10.580.10; -; 1.
DR   InterPro; IPR016142; Citrate_synth-like_lrg_a-sub.
DR   InterPro; IPR016143; Citrate_synth-like_sm_a-sub.
DR   InterPro; IPR002020; Citrate_synthase.
DR   InterPro; IPR019810; Citrate_synthase_AS.
DR   InterPro; IPR024176; Citrate_synthase_bac-typ.
DR   InterPro; IPR036969; Citrate_synthase_sf.
DR   InterPro; IPR010953; Citrate_synthase_typ-I.
DR   Pfam; PF00285; Citrate_synt; 1.
DR   PIRSF; PIRSF001369; Citrate_synth; 1.
DR   PRINTS; PR00143; CITRTSNTHASE.
DR   SUPFAM; SSF48256; SSF48256; 1.
DR   TIGRFAMs; TIGR01798; cit_synth_I; 1.
DR   PROSITE; PS00480; CITRATE_SYNTHASE; 1.
PE   3: Inferred from homology;
KW   Transferase; Tricarboxylic acid cycle.
FT   CHAIN           <1..>321
FT                   /note="Citrate synthase"
FT                   /id="PRO_0000169931"
FT   ACT_SITE        248
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10117"
FT   ACT_SITE        306
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10117"
FT   CONFLICT        229
FT                   /note="E -> Q (in Ref. 2; AAA74976)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        270
FT                   /note="Q -> K (in Ref. 2; AAA74976)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        277
FT                   /note="D -> N (in Ref. 2; AAA74976)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        280
FT                   /note="L -> F (in Ref. 2; AAA74976)"
FT                   /evidence="ECO:0000305"
FT   NON_TER         1
FT   NON_TER         321
SQ   SEQUENCE   321 AA;  36372 MW;  D61068C82AB6B550 CRC64;
     YIDGDEGILL YHGYSIDQLA ENGDFLETCY LLLYGELPNK QQKIDFDRCI MRHTMVHEQF
     ARFFHGFRRD SHPMAVMVAC LGAMSAFYHD SINITDPQQR MIASIRLISK VPTLAAMAYK
     YSIGQPFVYP RNDLNYATNF LHMCFSVPCE EHKISPVIAR AMDRIFTLHA DHEQNASTST
     VRLAGSSGAN PYACIAAGVA CLWGPAHGGA NEACLKMLQE IGSVKKIPEF IARAKDKNDP
     FRLMGFGHRV YKNYDPRAKI MQKTCHEVLQ ELNIQDDPLL DIAMELEHIA LNDEYFINKK
     LYPNVDFYSG ITLKALGFPT E