ID   CISY_BARHE              Reviewed;         431 AA.
AC   P51033;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   03-AUG-2022, entry version 119.
DE   RecName: Full=Citrate synthase;
DE            EC=2.3.3.16;
GN   Name=gltA; OrderedLocusNames=BH06380;
OS   Bartonella henselae (strain ATCC 49882 / DSM 28221 / Houston 1)
OS   (Rochalimaea henselae).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Bartonellaceae; Bartonella.
OX   NCBI_TaxID=283166;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 49882 / DSM 28221 / Houston 1;
RX   PubMed=7545181; DOI=10.1128/jcm.33.7.1797-1803.1995;
RA   Norman A.F., Regnery R., Jameson P., Greene C., Krause D.C.;
RT   "Differentiation of Bartonella-like isolates at the species level by PCR-
RT   restriction fragment length polymorphism in the citrate synthase gene.";
RL   J. Clin. Microbiol. 33:1797-1803(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 49882 / DSM 28221 / Houston 1;
RX   PubMed=15210978; DOI=10.1073/pnas.0305659101;
RA   Alsmark U.C.M., Frank A.C., Karlberg E.O., Legault B.-A., Ardell D.H.,
RA   Canbaeck B., Eriksson A.-S., Naeslund A.K., Handley S.A., Huvet M.,
RA   La Scola B., Holmberg M., Andersson S.G.E.;
RT   "The louse-borne human pathogen Bartonella quintana is a genomic derivative
RT   of the zoonotic agent Bartonella henselae.";
RL   Proc. Natl. Acad. Sci. U.S.A. 101:9716-9721(2004).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=acetyl-CoA + H2O + oxaloacetate = citrate + CoA + H(+);
CC         Xref=Rhea:RHEA:16845, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:16452, ChEBI:CHEBI:16947, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57288; EC=2.3.3.16; Evidence={ECO:0000255|PROSITE-
CC         ProRule:PRU10117};
CC   -!- PATHWAY: Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate
CC       from oxaloacetate: step 1/2.
CC   -!- MISCELLANEOUS: Citrate synthase is found in nearly all cells capable of
CC       oxidative metabolism.
CC   -!- SIMILARITY: Belongs to the citrate synthase family. {ECO:0000305}.
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DR   EMBL; L38987; AAA87325.1; -; Genomic_DNA.
DR   EMBL; BX897699; CAF27442.1; -; Genomic_DNA.
DR   PIR; I40044; I40044.
DR   RefSeq; WP_011180562.1; NZ_LRIJ02000001.1.
DR   AlphaFoldDB; P51033; -.
DR   SMR; P51033; -.
DR   STRING; 283166.BH06380; -.
DR   PaxDb; P51033; -.
DR   PRIDE; P51033; -.
DR   EnsemblBacteria; CAF27442; CAF27442; BH06380.
DR   GeneID; 64156917; -.
DR   KEGG; bhe:BH06380; -.
DR   eggNOG; COG0372; Bacteria.
DR   OMA; TVGWCAQ; -.
DR   UniPathway; UPA00223; UER00717.
DR   Proteomes; UP000000421; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:InterPro.
DR   GO; GO:0004108; F:citrate (Si)-synthase activity; IEA:InterPro.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-UniPathway.
DR   CDD; cd06114; EcCS_like; 1.
DR   Gene3D; 1.10.230.10; -; 1.
DR   Gene3D; 1.10.580.10; -; 1.
DR   InterPro; IPR016142; Citrate_synth-like_lrg_a-sub.
DR   InterPro; IPR016143; Citrate_synth-like_sm_a-sub.
DR   InterPro; IPR002020; Citrate_synthase.
DR   InterPro; IPR019810; Citrate_synthase_AS.
DR   InterPro; IPR024176; Citrate_synthase_bac-typ.
DR   InterPro; IPR036969; Citrate_synthase_sf.
DR   InterPro; IPR010953; Citrate_synthase_typ-I.
DR   Pfam; PF00285; Citrate_synt; 1.
DR   PIRSF; PIRSF001369; Citrate_synth; 1.
DR   PRINTS; PR00143; CITRTSNTHASE.
DR   SUPFAM; SSF48256; SSF48256; 1.
DR   TIGRFAMs; TIGR01798; cit_synth_I; 1.
DR   PROSITE; PS00480; CITRATE_SYNTHASE; 1.
PE   3: Inferred from homology;
KW   Transferase; Tricarboxylic acid cycle.
FT   CHAIN           1..431
FT                   /note="Citrate synthase"
FT                   /id="PRO_0000169934"
FT   ACT_SITE        306
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10117"
FT   ACT_SITE        364
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10117"
SQ   SEQUENCE   431 AA;  48802 MW;  DD40B8DEA70C2102 CRC64;
     MSKNKAHITV NDKKIELSVR KGTLGPDVIE IASLYKETDT FTYDPGFTST ASCESKITYI
     DGNEGILLYR GYPIDQLAEK GDFLESCYLL LYGELPTKQE KIDFDRCIMQ HTMVHEQFAR
     FFHGFRRDSH PMAVMIACLG AMSAFYHDSI DITDPQQRMI ASIRLISKVP TLAAMAYKYS
     IGQAFVYPRN DLSYAANFLR MCFSVPCEEY KINPVLTRAM DRIFTLHADH EQNASTSTVR
     LAGSSGANPF ACIAAGVACL WGPAHGGANE ACLKMLQEIG SVERIPEFIA RAKDKNDSFR
     LMGFGHRVYK NYDPRAKIMQ QTCHEVLKEL NIQNDPLLDI AITLENIALN DEYFIEKKLY
     PNVDFYSGIT LKALGFPTEM FTVLFALARS VGWVAQWKEM IEDPAQKISR PRQLYTGYAA
     REYIPIDKRV N