ID   CISY_BRADU              Reviewed;         434 AA.
AC   P94325;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   28-FEB-2003, sequence version 2.
DT   25-MAY-2022, entry version 116.
DE   RecName: Full=Citrate synthase;
DE            EC=2.3.3.16;
GN   Name=gltA; OrderedLocusNames=blr4839;
OS   Bradyrhizobium diazoefficiens (strain JCM 10833 / BCRC 13528 / IAM 13628 /
OS   NBRC 14792 / USDA 110).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Bradyrhizobiaceae; Bradyrhizobium.
OX   NCBI_TaxID=224911;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=61A152;
RA   Levier K., Guerinot M.L.;
RT   "Cloning of the Bradyrhizobium japonicum gltA gene encoding a central
RT   enzyme of the TCA cycle.";
RL   Submitted (OCT-1996) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=JCM 10833 / BCRC 13528 / IAM 13628 / NBRC 14792 / USDA 110;
RX   PubMed=12597275; DOI=10.1093/dnares/9.6.189;
RA   Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S.,
RA   Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M.,
RA   Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S.;
RT   "Complete genomic sequence of nitrogen-fixing symbiotic bacterium
RT   Bradyrhizobium japonicum USDA110.";
RL   DNA Res. 9:189-197(2002).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=acetyl-CoA + H2O + oxaloacetate = citrate + CoA + H(+);
CC         Xref=Rhea:RHEA:16845, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:16452, ChEBI:CHEBI:16947, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57288; EC=2.3.3.16; Evidence={ECO:0000255|PROSITE-
CC         ProRule:PRU10117};
CC   -!- PATHWAY: Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate
CC       from oxaloacetate: step 1/2.
CC   -!- SIMILARITY: Belongs to the citrate synthase family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; U76375; AAB39736.1; -; Genomic_DNA.
DR   EMBL; BA000040; BAC50104.1; -; Genomic_DNA.
DR   RefSeq; NP_771479.1; NC_004463.1.
DR   RefSeq; WP_011087607.1; NZ_CP011360.1.
DR   AlphaFoldDB; P94325; -.
DR   SMR; P94325; -.
DR   STRING; 224911.27353103; -.
DR   EnsemblBacteria; BAC50104; BAC50104; BAC50104.
DR   GeneID; 64024592; -.
DR   KEGG; bja:blr4839; -.
DR   PATRIC; fig|224911.44.peg.4684; -.
DR   eggNOG; COG0372; Bacteria.
DR   HOGENOM; CLU_025068_0_0_5; -.
DR   InParanoid; P94325; -.
DR   OMA; TVGWCAQ; -.
DR   PhylomeDB; P94325; -.
DR   UniPathway; UPA00223; UER00717.
DR   Proteomes; UP000002526; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:InterPro.
DR   GO; GO:0004108; F:citrate (Si)-synthase activity; IEA:InterPro.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-UniPathway.
DR   CDD; cd06114; EcCS_like; 1.
DR   Gene3D; 1.10.230.10; -; 1.
DR   Gene3D; 1.10.580.10; -; 1.
DR   InterPro; IPR016142; Citrate_synth-like_lrg_a-sub.
DR   InterPro; IPR016143; Citrate_synth-like_sm_a-sub.
DR   InterPro; IPR002020; Citrate_synthase.
DR   InterPro; IPR019810; Citrate_synthase_AS.
DR   InterPro; IPR024176; Citrate_synthase_bac-typ.
DR   InterPro; IPR036969; Citrate_synthase_sf.
DR   InterPro; IPR010953; Citrate_synthase_typ-I.
DR   Pfam; PF00285; Citrate_synt; 1.
DR   PIRSF; PIRSF001369; Citrate_synth; 1.
DR   PRINTS; PR00143; CITRTSNTHASE.
DR   SUPFAM; SSF48256; SSF48256; 1.
DR   TIGRFAMs; TIGR01798; cit_synth_I; 1.
DR   PROSITE; PS00480; CITRATE_SYNTHASE; 1.
PE   3: Inferred from homology;
KW   Reference proteome; Transferase; Tricarboxylic acid cycle.
FT   CHAIN           1..434
FT                   /note="Citrate synthase"
FT                   /id="PRO_0000169939"
FT   ACT_SITE        310
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10117"
FT   ACT_SITE        368
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10117"
FT   CONFLICT        210
FT                   /note="P -> S (in Ref. 1; AAB39736)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        281
FT                   /note="A -> G (in Ref. 1; AAB39736)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        346
FT                   /note="M -> L (in Ref. 1; AAB39736)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   434 AA;  48193 MW;  DEB33EC2B4BACDB4 CRC64;
     MDAKASNKTA TLTVGNKNYD LPIHSGSVGP DVIDIGKLYG QSGLFTYDPG FTSTASCQSK
     ITYIDGDAGV LEYRGYPIEQ LAENGDFLET CYLLLYGNLP TAAQKKDFDD RVIHHTMVHE
     QMARFFQGFR RDAHPMAVMV ASVGALAAFY HDSTDINDPK QRMIASMRMI AKIPTLAAMA
     YKYTIGQPFV YPKNSLKFAE NFLHMCFAVP CEEYKINPVL ADALDKIFIL HADHEQNAST
     STVRIAGSSG ANPFACIAAG IACLWGPAHG GANEAALAML AEIGSVDKIP EFIAKVKDKN
     SEVRLMGFGH RVYKNYDPRA KIMQKMCHAV LKETGHGDDP MLKVAMELEK IALSDQYFID
     RKLYPNVDFY SGITLKAMGF PVSMFTVLFA VARTVGWISQ WSEMIEDPQQ KIGRPRQLYT
     GVTRRDYVAI KDRK