ID   CISY_CAEBR              Reviewed;         468 AA.
AC   Q61JF9; A8X9P6;
DT   26-JUN-2007, integrated into UniProtKB/Swiss-Prot.
DT   23-NOV-2004, sequence version 1.
DT   03-AUG-2022, entry version 84.
DE   RecName: Full=Probable citrate synthase, mitochondrial;
DE            EC=2.3.3.16;
DE   Flags: Precursor;
GN   Name=cts-1; ORFNames=CBG09810;
OS   Caenorhabditis briggsae.
OC   Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC   Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC   Caenorhabditis.
OX   NCBI_TaxID=6238;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AF16;
RX   PubMed=14624247; DOI=10.1371/journal.pbio.0000045;
RA   Stein L.D., Bao Z., Blasiar D., Blumenthal T., Brent M.R., Chen N.,
RA   Chinwalla A., Clarke L., Clee C., Coghlan A., Coulson A., D'Eustachio P.,
RA   Fitch D.H.A., Fulton L.A., Fulton R.E., Griffiths-Jones S., Harris T.W.,
RA   Hillier L.W., Kamath R., Kuwabara P.E., Mardis E.R., Marra M.A.,
RA   Miner T.L., Minx P., Mullikin J.C., Plumb R.W., Rogers J., Schein J.E.,
RA   Sohrmann M., Spieth J., Stajich J.E., Wei C., Willey D., Wilson R.K.,
RA   Durbin R.M., Waterston R.H.;
RT   "The genome sequence of Caenorhabditis briggsae: a platform for comparative
RT   genomics.";
RL   PLoS Biol. 1:166-192(2003).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=acetyl-CoA + H2O + oxaloacetate = citrate + CoA + H(+);
CC         Xref=Rhea:RHEA:16845, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:16452, ChEBI:CHEBI:16947, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57288; EC=2.3.3.16; Evidence={ECO:0000255|PROSITE-
CC         ProRule:PRU10117};
CC   -!- PATHWAY: Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate
CC       from oxaloacetate: step 1/2.
CC   -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion matrix {ECO:0000250}.
CC   -!- MISCELLANEOUS: Citrate synthase is found in nearly all cells capable of
CC       oxidative metabolism.
CC   -!- SIMILARITY: Belongs to the citrate synthase family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; HE601459; CAP29361.1; -; Genomic_DNA.
DR   RefSeq; XP_002641515.1; XM_002641469.1.
DR   AlphaFoldDB; Q61JF9; -.
DR   SMR; Q61JF9; -.
DR   STRING; 6238.CBG09810; -.
DR   EnsemblMetazoa; CBG09810.1; CBG09810.1; WBGene00031330.
DR   GeneID; 8583506; -.
DR   KEGG; cbr:CBG_09810; -.
DR   CTD; 8583506; -.
DR   WormBase; CBG09810; CBP02471; WBGene00031330; Cbr-cts-1.
DR   eggNOG; KOG2617; Eukaryota.
DR   HOGENOM; CLU_022049_2_1_1; -.
DR   InParanoid; Q61JF9; -.
DR   OMA; TVGWCAQ; -.
DR   OrthoDB; 1131452at2759; -.
DR   UniPathway; UPA00223; UER00717.
DR   Proteomes; UP000008549; Chromosome III.
DR   GO; GO:0005759; C:mitochondrial matrix; ISS:UniProtKB.
DR   GO; GO:0004108; F:citrate (Si)-synthase activity; ISS:UniProtKB.
DR   GO; GO:0005975; P:carbohydrate metabolic process; ISS:UniProtKB.
DR   GO; GO:0006101; P:citrate metabolic process; IEA:InterPro.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IBA:GO_Central.
DR   Gene3D; 1.10.230.10; -; 1.
DR   Gene3D; 1.10.580.10; -; 1.
DR   InterPro; IPR016142; Citrate_synth-like_lrg_a-sub.
DR   InterPro; IPR016143; Citrate_synth-like_sm_a-sub.
DR   InterPro; IPR002020; Citrate_synthase.
DR   InterPro; IPR019810; Citrate_synthase_AS.
DR   InterPro; IPR010109; Citrate_synthase_euk.
DR   InterPro; IPR036969; Citrate_synthase_sf.
DR   PANTHER; PTHR11739; PTHR11739; 1.
DR   Pfam; PF00285; Citrate_synt; 1.
DR   PRINTS; PR00143; CITRTSNTHASE.
DR   SUPFAM; SSF48256; SSF48256; 1.
DR   TIGRFAMs; TIGR01793; cit_synth_euk; 1.
DR   PROSITE; PS00480; CITRATE_SYNTHASE; 1.
PE   3: Inferred from homology;
KW   Mitochondrion; Reference proteome; Transferase; Transit peptide;
KW   Tricarboxylic acid cycle.
FT   TRANSIT         1..?
FT                   /note="Mitochondrion"
FT                   /evidence="ECO:0000255"
FT   CHAIN           ?..468
FT                   /note="Probable citrate synthase, mitochondrial"
FT                   /id="PRO_0000291600"
FT   ACT_SITE        303
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10117"
FT   ACT_SITE        349
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10117"
FT   ACT_SITE        404
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10117"
SQ   SEQUENCE   468 AA;  51615 MW;  9BF4AEAFBB4D1DDE CRC64;
     MPITGTVLRR FITKGVIPMC QVAPLSTSAE GSTNLKEVLS KKIPAHNAKV KSFRAEHGNT
     VVQSVNIDMI YGGMRSMKGM VTETSVLDPE EGIRFRGYSI PECQKLLPKA KGGEEPLPEA
     IWWLLCTGDV PSEAQTAAIT KEWNARADLP THVVRMLDNF PDNLHPMAQF IAAIAALNNE
     SKFAGAYARG VAKASYWEYA YEDSMDLLAK LPTVAAIIYR NLYRDGSAVS VIDPKKDWSA
     NFSSMLGYDD PLFAELMRLY LVIHSDHEGG NVSAHTSHLV GSALSDPYLA FSAAMAGLAG
     PLHGLANQEV LVFLNKIVGE IGFNYTEEQL KEWVWKHLKS GQVVPGYGHA VLRKTDPRYE
     CQREFALKHL PNDDLFKLVS TLYKITPGIL LEQGKAKNPW PNVDSHSGVL LQYFGMTEMS
     FYTVLFGVSR ALGCLSQLIW ARGMGLPLER PKSHSTEGLI KLAMAAKK