ID   CISY_CAEEL              Reviewed;         468 AA.
AC   P34575;
DT   01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1994, sequence version 1.
DT   03-AUG-2022, entry version 143.
DE   RecName: Full=Probable citrate synthase, mitochondrial;
DE            EC=2.3.3.16;
DE   Flags: Precursor;
GN   Name=cts-1; ORFNames=T20G5.2;
OS   Caenorhabditis elegans.
OC   Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC   Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC   Caenorhabditis.
OX   NCBI_TaxID=6239;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Bristol N2;
RX   PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG   The C. elegans sequencing consortium;
RT   "Genome sequence of the nematode C. elegans: a platform for investigating
RT   biology.";
RL   Science 282:2012-2018(1998).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=acetyl-CoA + H2O + oxaloacetate = citrate + CoA + H(+);
CC         Xref=Rhea:RHEA:16845, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:16452, ChEBI:CHEBI:16947, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57288; EC=2.3.3.16; Evidence={ECO:0000255|PROSITE-
CC         ProRule:PRU10117};
CC   -!- PATHWAY: Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate
CC       from oxaloacetate: step 1/2.
CC   -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion matrix {ECO:0000250}.
CC   -!- MISCELLANEOUS: Citrate synthase is found in nearly all cells capable of
CC       oxidative metabolism.
CC   -!- SIMILARITY: Belongs to the citrate synthase family. {ECO:0000305}.
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DR   EMBL; Z30423; CAA83004.1; -; Genomic_DNA.
DR   PIR; S42370; S42370.
DR   RefSeq; NP_499264.1; NM_066863.6.
DR   AlphaFoldDB; P34575; -.
DR   SMR; P34575; -.
DR   BioGRID; 41631; 18.
DR   STRING; 6239.T20G5.2; -.
DR   World-2DPAGE; 0020:P34575; -.
DR   EPD; P34575; -.
DR   PaxDb; P34575; -.
DR   PeptideAtlas; P34575; -.
DR   EnsemblMetazoa; T20G5.2.1; T20G5.2.1; WBGene00000833.
DR   GeneID; 176437; -.
DR   KEGG; cel:CELE_T20G5.2; -.
DR   UCSC; T20G5.2.1; c. elegans.
DR   CTD; 176437; -.
DR   WormBase; T20G5.2; CE00513; WBGene00000833; cts-1.
DR   eggNOG; KOG2617; Eukaryota.
DR   GeneTree; ENSGT00390000006813; -.
DR   HOGENOM; CLU_022049_2_1_1; -.
DR   InParanoid; P34575; -.
DR   OMA; TVGWCAQ; -.
DR   OrthoDB; 1131452at2759; -.
DR   PhylomeDB; P34575; -.
DR   Reactome; R-CEL-71403; Citric acid cycle (TCA cycle).
DR   UniPathway; UPA00223; UER00717.
DR   PRO; PR:P34575; -.
DR   Proteomes; UP000001940; Chromosome III.
DR   Bgee; WBGene00000833; Expressed in adult organism and 4 other tissues.
DR   GO; GO:0005759; C:mitochondrial matrix; ISS:UniProtKB.
DR   GO; GO:0005739; C:mitochondrion; HDA:WormBase.
DR   GO; GO:0004108; F:citrate (Si)-synthase activity; ISS:UniProtKB.
DR   GO; GO:0005975; P:carbohydrate metabolic process; ISS:UniProtKB.
DR   GO; GO:0006101; P:citrate metabolic process; IEA:InterPro.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IBA:GO_Central.
DR   Gene3D; 1.10.230.10; -; 1.
DR   Gene3D; 1.10.580.10; -; 1.
DR   InterPro; IPR016142; Citrate_synth-like_lrg_a-sub.
DR   InterPro; IPR016143; Citrate_synth-like_sm_a-sub.
DR   InterPro; IPR002020; Citrate_synthase.
DR   InterPro; IPR019810; Citrate_synthase_AS.
DR   InterPro; IPR010109; Citrate_synthase_euk.
DR   InterPro; IPR036969; Citrate_synthase_sf.
DR   PANTHER; PTHR11739; PTHR11739; 1.
DR   Pfam; PF00285; Citrate_synt; 1.
DR   PRINTS; PR00143; CITRTSNTHASE.
DR   SUPFAM; SSF48256; SSF48256; 1.
DR   TIGRFAMs; TIGR01793; cit_synth_euk; 1.
DR   PROSITE; PS00480; CITRATE_SYNTHASE; 1.
PE   3: Inferred from homology;
KW   Mitochondrion; Reference proteome; Transferase; Transit peptide;
KW   Tricarboxylic acid cycle.
FT   TRANSIT         1..?
FT                   /note="Mitochondrion"
FT                   /evidence="ECO:0000255"
FT   CHAIN           ?..468
FT                   /note="Probable citrate synthase, mitochondrial"
FT                   /id="PRO_0000005470"
FT   ACT_SITE        303
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10117"
FT   ACT_SITE        349
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10117"
FT   ACT_SITE        404
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10117"
SQ   SEQUENCE   468 AA;  51540 MW;  CDE40CCA5729B9EA CRC64;
     MSLSGMAIRR LITKGVIPVC QVAPLSTSAE GSTNLKEVLS KKIPAHNAKV KSFRTEHGST
     VVQNVNIDMI YGGMRSMKGM VTETSVLDPE EGIRFRGYSI PECQKLLPKA KGGEEPLPEA
     IWWLLCTGDV PSEAQTAAIT KEWNARADLP THVVRMLDNF PDNLHPMAQF IAAIAALNNE
     SKFAGAYARG VAKASYWEYA YEDSMDLLAK LPTVAAIIYR NLYRDGSAVS VIDPKKDWSA
     NFSSMLGYDD PLFAELMRLY LVIHSDHEGG NVSAHTSHLV GSALSDPYLS FSAAMAGLAG
     PLHGLANQEV LVFLNKIVGE IGFNYTEEQL KEWVWKHLKS GQVVPGYGHA VLRKTDPRYE
     CQREFALKHL PNDDLFKLVS TLYKITPGIL LEQGKAKNPW PNVDSHSGVL LQYFGMTEMS
     FYTVLFGVSR ALGCLSQLIW ARGMGLPLER PKSHSTDGLI KLALAAKK