CISY_CITMA
ID CISY_CITMA Reviewed; 471 AA.
AC P49298;
DT 01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1996, sequence version 1.
DT 25-MAY-2022, entry version 92.
DE RecName: Full=Citrate synthase, mitochondrial;
DE EC=2.3.3.16;
DE Flags: Precursor;
GN Name=CIT;
OS Citrus maxima (Pomelo) (Citrus grandis).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Sapindales; Rutaceae; Aurantioideae; Citrus.
OX NCBI_TaxID=37334;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Siamese Sweet 2240;
RA Canel C.;
RL Thesis (1994), University of California Riverside, United States.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=acetyl-CoA + H2O + oxaloacetate = citrate + CoA + H(+);
CC Xref=Rhea:RHEA:16845, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:16452, ChEBI:CHEBI:16947, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:57288; EC=2.3.3.16; Evidence={ECO:0000255|PROSITE-
CC ProRule:PRU10117};
CC -!- PATHWAY: Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate
CC from oxaloacetate: step 1/2.
CC -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion matrix.
CC -!- MISCELLANEOUS: Citrate synthase is found in nearly all cells capable of
CC oxidative metabolism.
CC -!- SIMILARITY: Belongs to the citrate synthase family. {ECO:0000305}.
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DR EMBL; U19481; AAA82743.1; -; mRNA.
DR AlphaFoldDB; P49298; -.
DR SMR; P49298; -.
DR UniPathway; UPA00223; UER00717.
DR GO; GO:0005759; C:mitochondrial matrix; IEA:UniProtKB-SubCell.
DR GO; GO:0004108; F:citrate (Si)-synthase activity; IEA:InterPro.
DR GO; GO:0006101; P:citrate metabolic process; IEA:InterPro.
DR GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-UniPathway.
DR Gene3D; 1.10.230.10; -; 1.
DR Gene3D; 1.10.580.10; -; 1.
DR InterPro; IPR016142; Citrate_synth-like_lrg_a-sub.
DR InterPro; IPR016143; Citrate_synth-like_sm_a-sub.
DR InterPro; IPR002020; Citrate_synthase.
DR InterPro; IPR019810; Citrate_synthase_AS.
DR InterPro; IPR010109; Citrate_synthase_euk.
DR InterPro; IPR036969; Citrate_synthase_sf.
DR PANTHER; PTHR11739; PTHR11739; 1.
DR Pfam; PF00285; Citrate_synt; 1.
DR PRINTS; PR00143; CITRTSNTHASE.
DR SUPFAM; SSF48256; SSF48256; 1.
DR TIGRFAMs; TIGR01793; cit_synth_euk; 1.
DR PROSITE; PS00480; CITRATE_SYNTHASE; 1.
PE 2: Evidence at transcript level;
KW Mitochondrion; Transferase; Transit peptide; Tricarboxylic acid cycle.
FT TRANSIT 1..18
FT /note="Mitochondrion"
FT /evidence="ECO:0000255"
FT CHAIN 19..471
FT /note="Citrate synthase, mitochondrial"
FT /id="PRO_0000005486"
FT ACT_SITE 307
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10117"
FT ACT_SITE 353
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10117"
FT ACT_SITE 408
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10117"
SQ SEQUENCE 471 AA; 52183 MW; 8EE1E23F7E154806 CRC64;
MASLRSATAL SRLRSRAGQQ SNLSNSVRWL QMQSSADLDL HSQLKEMIPE QQERLKKVKS
DLGKAQLGNI TIDVVIGGMR GMTGLLWETS LLDPDEGIRF RGLSIPECQK LLPAAKPDGE
PLPEGLLWLL LTGKVPSKEQ VDGLSKELRD RATVPDYVYK AIDALPVSAH PMTQFASGVM
ALQVQSEFQE AYEKGIHKSK SWEPTSEDSL NLIARVPVVA AYVYQRIYKD GKIIPKDDSL
DYGGNFSHML GFDDPKMLEL MRLYVTIHSD HEGGNVSAHT GHLVASALSD PYLSFLAALN
GLAGPLHGLA NQEVLLWIKS VVDECGENVT TEQLKDYVWK TLNSGKVVPG FGHGVLRKTD
PRYTCQREFA LKHLPDDPLF QLVSKLYEVV PPILTKLGKV KNPWPNVDAH SGVLLNHFGL
AEARYYTVLF GVSRSLGICS QLIWDRALGL PLERPKSVTL DWIEKNCKKA A