ID   CISY_DANRE              Reviewed;         468 AA.
AC   Q7ZVY5;
DT   17-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   03-AUG-2022, entry version 110.
DE   RecName: Full=Citrate synthase, mitochondrial;
DE            EC=2.3.3.1;
DE   AltName: Full=Citrate (Si)-synthase;
DE   Flags: Precursor;
GN   Name=cs; ORFNames=zgc:55507;
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Danionidae; Danioninae; Danio.
OX   NCBI_TaxID=7955;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=AB;
RG   NIH - Zebrafish Gene Collection (ZGC) project;
RL   Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=acetyl-CoA + H2O + oxaloacetate = citrate + CoA + H(+);
CC         Xref=Rhea:RHEA:16845, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:16452, ChEBI:CHEBI:16947, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57288; EC=2.3.3.1; Evidence={ECO:0000255|PROSITE-
CC         ProRule:PRU10117};
CC   -!- PATHWAY: Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate
CC       from oxaloacetate: step 1/2.
CC   -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion matrix {ECO:0000250}.
CC   -!- MISCELLANEOUS: Citrate synthase is found in nearly all cells capable of
CC       oxidative metabolism.
CC   -!- SIMILARITY: Belongs to the citrate synthase family. {ECO:0000305}.
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DR   EMBL; BC045362; AAH45362.1; -; mRNA.
DR   RefSeq; NP_955892.1; NM_199598.1.
DR   AlphaFoldDB; Q7ZVY5; -.
DR   SMR; Q7ZVY5; -.
DR   STRING; 7955.ENSDARP00000094021; -.
DR   PaxDb; Q7ZVY5; -.
DR   PRIDE; Q7ZVY5; -.
DR   GeneID; 322339; -.
DR   KEGG; dre:322339; -.
DR   CTD; 1431; -.
DR   ZFIN; ZDB-GENE-030131-1058; cs.
DR   eggNOG; KOG2617; Eukaryota.
DR   InParanoid; Q7ZVY5; -.
DR   OrthoDB; 1131452at2759; -.
DR   PhylomeDB; Q7ZVY5; -.
DR   Reactome; R-DRE-71403; Citric acid cycle (TCA cycle).
DR   UniPathway; UPA00223; UER00717.
DR   PRO; PR:Q7ZVY5; -.
DR   Proteomes; UP000000437; Genome assembly.
DR   Proteomes; UP000814640; Unplaced.
DR   GO; GO:0005759; C:mitochondrial matrix; ISS:UniProtKB.
DR   GO; GO:0004108; F:citrate (Si)-synthase activity; ISS:UniProtKB.
DR   GO; GO:0005975; P:carbohydrate metabolic process; ISS:UniProtKB.
DR   GO; GO:0006101; P:citrate metabolic process; IEA:InterPro.
DR   GO; GO:0014823; P:response to activity; IDA:ZFIN.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IBA:GO_Central.
DR   Gene3D; 1.10.230.10; -; 1.
DR   Gene3D; 1.10.580.10; -; 1.
DR   InterPro; IPR016142; Citrate_synth-like_lrg_a-sub.
DR   InterPro; IPR016143; Citrate_synth-like_sm_a-sub.
DR   InterPro; IPR002020; Citrate_synthase.
DR   InterPro; IPR019810; Citrate_synthase_AS.
DR   InterPro; IPR010109; Citrate_synthase_euk.
DR   InterPro; IPR036969; Citrate_synthase_sf.
DR   PANTHER; PTHR11739; PTHR11739; 1.
DR   Pfam; PF00285; Citrate_synt; 1.
DR   PRINTS; PR00143; CITRTSNTHASE.
DR   SUPFAM; SSF48256; SSF48256; 1.
DR   TIGRFAMs; TIGR01793; cit_synth_euk; 1.
DR   PROSITE; PS00480; CITRATE_SYNTHASE; 1.
PE   2: Evidence at transcript level;
KW   Mitochondrion; Reference proteome; Transferase; Transit peptide;
KW   Tricarboxylic acid cycle.
FT   TRANSIT         1..30
FT                   /note="Mitochondrion"
FT                   /evidence="ECO:0000250"
FT   CHAIN           31..468
FT                   /note="Citrate synthase, mitochondrial"
FT                   /id="PRO_0000253902"
FT   ACT_SITE        303
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10117"
FT   ACT_SITE        349
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10117"
FT   ACT_SITE        404
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10117"
SQ   SEQUENCE   468 AA;  51751 MW;  81F3D0260317065F CRC64;
     MSFLSISRLA PRLLSSKNAA CVVVAARNAS ASTNLKDVLS DLVPKEQSRI KNFKQQYGKT
     SIGQITVDMV YGGMRGVKGL VYETSVLDPD EGIRFRGYSI PECQQLLPKA PGGEEPLPEG
     LFWLLVTGQV PTEEQVSWLS KEWAKRAALP SHVVTMLDNF PTNLHPMSQF SAAITALNSE
     SSFARAYSEG VNKAKYWEFV YEDSMDLIAK LPCVAAKIYR NLYREGSSIG AIDSNLDWSH
     NFTNMLGYTE PQFTELMRLY LTIHSDHEGG NVSAHTSHLV GSALSDPYLS FSAAMNGLAG
     PLHGLANQEV LVWLTALQKE LGGEVSDEKM RDYIWNTLKS GRVVPGYGHA VLRKTDPRYT
     CQREFALKHL PNDPMFKLVA QLYKIVPNVL LEQGKAKNPW PNVDAHSGVL LQYYGMTEMN
     YYTVLFGVSR ALGVLAQLVW SRALGFPLER PKSMSTDGLM ALVGAKSG