CISY_FRAAN
ID CISY_FRAAN Reviewed; 469 AA.
AC P83372;
DT 01-NOV-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 25-MAY-2022, entry version 80.
DE RecName: Full=Citrate synthase, mitochondrial;
DE EC=2.3.3.16;
DE Flags: Precursor;
GN Name=MCSI;
OS Fragaria ananassa (Strawberry) (Fragaria chiloensis x Fragaria virginiana).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Rosales; Rosaceae; Rosoideae; Potentilleae; Fragariinae;
OC Fragaria.
OX NCBI_TaxID=3747 {ECO:0000305};
RN [1] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE, AND PROTEIN SEQUENCE OF 29-46.
RC STRAIN=cv. Elsanta; TISSUE=Fruit;
RX PubMed=15086813; DOI=10.1111/j.0031-9317.2004.00302.x;
RA Iannetta P.P.M., Escobar N.M., Ross H.A., Souleyre E.J., Hancock R.D.,
RA Witte C.P., Davies H.V.;
RT "Identification, cloning and expression analysis of strawberry (Fragaria x
RT ananassa) mitochondrial citrate synthase and mitochondrial malate
RT dehydrogenase.";
RL Physiol. Plantarum 121:15-26(2004).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=acetyl-CoA + H2O + oxaloacetate = citrate + CoA + H(+);
CC Xref=Rhea:RHEA:16845, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:16452, ChEBI:CHEBI:16947, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:57288; EC=2.3.3.16; Evidence={ECO:0000255|PROSITE-
CC ProRule:PRU10117};
CC -!- PATHWAY: Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate
CC from oxaloacetate: step 1/2.
CC -!- SUBUNIT: Homodimer. {ECO:0000250|UniProtKB:P00889}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion matrix.
CC -!- MISCELLANEOUS: Citrate synthase is found in nearly all cells capable of
CC oxidative metabolism. {ECO:0000250|UniProtKB:P00889}.
CC -!- SIMILARITY: Belongs to the citrate synthase family. {ECO:0000305}.
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DR AlphaFoldDB; P83372; -.
DR SMR; P83372; -.
DR BRENDA; 2.3.3.16; 2320.
DR UniPathway; UPA00223; UER00717.
DR GO; GO:0005759; C:mitochondrial matrix; IEA:UniProtKB-SubCell.
DR GO; GO:0004108; F:citrate (Si)-synthase activity; IEA:InterPro.
DR GO; GO:0006101; P:citrate metabolic process; IEA:InterPro.
DR GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-UniPathway.
DR Gene3D; 1.10.230.10; -; 1.
DR Gene3D; 1.10.580.10; -; 1.
DR InterPro; IPR016142; Citrate_synth-like_lrg_a-sub.
DR InterPro; IPR016143; Citrate_synth-like_sm_a-sub.
DR InterPro; IPR002020; Citrate_synthase.
DR InterPro; IPR019810; Citrate_synthase_AS.
DR InterPro; IPR010109; Citrate_synthase_euk.
DR InterPro; IPR036969; Citrate_synthase_sf.
DR PANTHER; PTHR11739; PTHR11739; 1.
DR Pfam; PF00285; Citrate_synt; 1.
DR PRINTS; PR00143; CITRTSNTHASE.
DR SUPFAM; SSF48256; SSF48256; 1.
DR TIGRFAMs; TIGR01793; cit_synth_euk; 1.
DR PROSITE; PS00480; CITRATE_SYNTHASE; 1.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Mitochondrion; Transferase; Transit peptide;
KW Tricarboxylic acid cycle.
FT TRANSIT 1..28
FT /note="Mitochondrion"
FT /evidence="ECO:0000269|PubMed:15086813"
FT CHAIN 29..469
FT /note="Citrate synthase, mitochondrial"
FT /id="PRO_0000005488"
FT ACT_SITE 304
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10117"
FT ACT_SITE 350
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10117"
FT ACT_SITE 405
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10117"
SQ SEQUENCE 469 AA; 52283 MW; CD80AAED06246F65 CRC64;
MAFFRTVTKL RSRLGQPPSL RDSVRCLQTQ ASSDLDLHSQ LKELIPEQQE RLKKLKKEHG
KVQLGTITVD MVIGGMRGMT GLLWETSLLD PDEGIRFRGL SIPECQKVLP GATPGGEPLP
EGLLWLLLTG KVPSRSKNMH YPVNNGVVPK FQIMCSRPLM LCLLEHIPMT QFTTGVMALQ
VQSEFQKAYD KGIPKSRYWE PTYEDSLSLI AQLPVVASYV YRRIYKGGRM IPVDDSLDYG
GNFSHLLGFD DHKMQELMRL YVTIHSDHEG GNVSAHTGHL VASALSDPFL SFAAALNGLA
GPLHGLANQE VLLWIKSVVD ECGENITKDQ LKDYVWKTLN SGKVVPGFGH GVLRKTDPRY
TCQREFALKH LPDDPLFRLV SKLYDVVPPI LTELGKVKNP WPNVDAHSGV LLNHFGLTEA
RYFTVLFGVS RSIGIGSQLI WDRALGLPLE RPKSVTMESL ESFCKKAAS