CISY_KAJAU
ID CISY_KAJAU Reviewed; 469 AA.
AC Q6S9V5;
DT 17-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 25-MAY-2022, entry version 68.
DE RecName: Full=Citrate synthase, mitochondrial;
DE EC=2.3.3.1;
DE AltName: Full=Citrate (Si)-synthase;
DE Flags: Precursor;
GN Name=cs;
OS Kajikia audax (Striped marlin) (Tetrapturus audax).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Neoteleostei; Acanthomorphata;
OC Carangaria; Istiophoriformes; Kajikia.
OX NCBI_TaxID=13721;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Red muscle;
RX PubMed=15374817; DOI=10.1152/ajpregu.00152.2004;
RA Dalziel A.C., Moore S.E., Moyes C.D.;
RT "Mitochondrial enzyme content in the muscles of high-performance fish:
RT evolution and variation among fiber types.";
RL Am. J. Physiol. 288:R163-R172(2005).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=acetyl-CoA + H2O + oxaloacetate = citrate + CoA + H(+);
CC Xref=Rhea:RHEA:16845, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:16452, ChEBI:CHEBI:16947, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:57288; EC=2.3.3.1; Evidence={ECO:0000255|PROSITE-
CC ProRule:PRU10117};
CC -!- PATHWAY: Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate
CC from oxaloacetate: step 1/2.
CC -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion matrix {ECO:0000250}.
CC -!- MISCELLANEOUS: Citrate synthase is found in nearly all cells capable of
CC oxidative metabolism.
CC -!- SIMILARITY: Belongs to the citrate synthase family. {ECO:0000305}.
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DR EMBL; AY461852; AAR98862.1; -; mRNA.
DR AlphaFoldDB; Q6S9V5; -.
DR SMR; Q6S9V5; -.
DR UniPathway; UPA00223; UER00717.
DR GO; GO:0005759; C:mitochondrial matrix; ISS:UniProtKB.
DR GO; GO:0004108; F:citrate (Si)-synthase activity; ISS:UniProtKB.
DR GO; GO:0005975; P:carbohydrate metabolic process; ISS:UniProtKB.
DR GO; GO:0006101; P:citrate metabolic process; IEA:InterPro.
DR GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-UniPathway.
DR Gene3D; 1.10.230.10; -; 1.
DR Gene3D; 1.10.580.10; -; 1.
DR InterPro; IPR016142; Citrate_synth-like_lrg_a-sub.
DR InterPro; IPR016143; Citrate_synth-like_sm_a-sub.
DR InterPro; IPR002020; Citrate_synthase.
DR InterPro; IPR019810; Citrate_synthase_AS.
DR InterPro; IPR010109; Citrate_synthase_euk.
DR InterPro; IPR036969; Citrate_synthase_sf.
DR PANTHER; PTHR11739; PTHR11739; 1.
DR Pfam; PF00285; Citrate_synt; 1.
DR PRINTS; PR00143; CITRTSNTHASE.
DR SUPFAM; SSF48256; SSF48256; 1.
DR TIGRFAMs; TIGR01793; cit_synth_euk; 1.
DR PROSITE; PS00480; CITRATE_SYNTHASE; 1.
PE 2: Evidence at transcript level;
KW Mitochondrion; Transferase; Transit peptide; Tricarboxylic acid cycle.
FT TRANSIT 1..30
FT /note="Mitochondrion"
FT /evidence="ECO:0000250"
FT CHAIN 31..469
FT /note="Citrate synthase, mitochondrial"
FT /id="PRO_0000253904"
FT ACT_SITE 304
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10117"
FT ACT_SITE 350
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10117"
FT ACT_SITE 405
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10117"
SQ SEQUENCE 469 AA; 52213 MW; 5DEB928051938CBB CRC64;
MSFLTVSRLA PKLLNSKNAT YFLVAARNAS ASSTNLKDVL ADLIPKEQTR IKNFKQQYGK
TNIGQITVDM VYGGMRGMKG LVYETSVLDP EEGIRFRGYS IPECQKLLPK APGGEEPLPE
GLFWLLVTGQ VPTEEQVNWV SKEWAKRAAL PSHVVTMLDN FPTNLHPMSQ FSAAITALNS
ESSFARAYSE GVHKSKYWEF IYEDSMDLIA KLPCIAAKIY RNLYREGSSI GAIDSNLDWS
HNFTNMLGYS EPQFTELMRL YLTIHSDHEG GNVSAHTSHL VGSALSDPYL SFSAAMNGLA
GPLHGLANQE VLVWLTALQK ELGGEVSDER MRDYIWNTLK SGRVVPGYGH AVLRKTDPRY
TCQREFALKH LPNDPMFKLV AQLYKIVPNV LLEQGRAKNP WPNVDAHSGV LLQYYGMTEM
NYYTVLFGVS RALGVLAQLV WSRALGFPLE RPKSMSSDGL MTLVGAKSG