CISY_RHIME
ID CISY_RHIME Reviewed; 429 AA.
AC O33915;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT 30-MAY-2000, sequence version 2.
DT 25-MAY-2022, entry version 119.
DE RecName: Full=Citrate synthase;
DE EC=2.3.3.16;
GN Name=gltA; OrderedLocusNames=R01509; ORFNames=SMc02087;
OS Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium
OS meliloti).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC Rhizobiaceae; Sinorhizobium/Ensifer group; Sinorhizobium.
OX NCBI_TaxID=266834;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=104A14;
RA Mortimer M.W., McDermott T.R., Kahn M.L.;
RT "Isolation and characterization of a gene coding for citrate synthase from
RT Rhizobium meliloti.";
RL Submitted (OCT-1996) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=1021;
RX PubMed=11481430; DOI=10.1073/pnas.161294398;
RA Capela D., Barloy-Hubler F., Gouzy J., Bothe G., Ampe F., Batut J.,
RA Boistard P., Becker A., Boutry M., Cadieu E., Dreano S., Gloux S.,
RA Godrie T., Goffeau A., Kahn D., Kiss E., Lelaure V., Masuy D., Pohl T.,
RA Portetelle D., Puehler A., Purnelle B., Ramsperger U., Renard C.,
RA Thebault P., Vandenbol M., Weidner S., Galibert F.;
RT "Analysis of the chromosome sequence of the legume symbiont Sinorhizobium
RT meliloti strain 1021.";
RL Proc. Natl. Acad. Sci. U.S.A. 98:9877-9882(2001).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=1021;
RX PubMed=11474104; DOI=10.1126/science.1060966;
RA Galibert F., Finan T.M., Long S.R., Puehler A., Abola P., Ampe F.,
RA Barloy-Hubler F., Barnett M.J., Becker A., Boistard P., Bothe G.,
RA Boutry M., Bowser L., Buhrmester J., Cadieu E., Capela D., Chain P.,
RA Cowie A., Davis R.W., Dreano S., Federspiel N.A., Fisher R.F., Gloux S.,
RA Godrie T., Goffeau A., Golding B., Gouzy J., Gurjal M., Hernandez-Lucas I.,
RA Hong A., Huizar L., Hyman R.W., Jones T., Kahn D., Kahn M.L., Kalman S.,
RA Keating D.H., Kiss E., Komp C., Lelaure V., Masuy D., Palm C., Peck M.C.,
RA Pohl T.M., Portetelle D., Purnelle B., Ramsperger U., Surzycki R.,
RA Thebault P., Vandenbol M., Vorhoelter F.J., Weidner S., Wells D.H.,
RA Wong K., Yeh K.-C., Batut J.;
RT "The composite genome of the legume symbiont Sinorhizobium meliloti.";
RL Science 293:668-672(2001).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=acetyl-CoA + H2O + oxaloacetate = citrate + CoA + H(+);
CC Xref=Rhea:RHEA:16845, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:16452, ChEBI:CHEBI:16947, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:57288; EC=2.3.3.16; Evidence={ECO:0000255|PROSITE-
CC ProRule:PRU10117};
CC -!- PATHWAY: Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate
CC from oxaloacetate: step 1/2.
CC -!- SIMILARITY: Belongs to the citrate synthase family. {ECO:0000305}.
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DR EMBL; U75365; AAB82405.1; -; Genomic_DNA.
DR EMBL; AL591688; CAC46088.1; -; Genomic_DNA.
DR RefSeq; NP_385615.1; NC_003047.1.
DR RefSeq; WP_010969265.1; NC_003047.1.
DR AlphaFoldDB; O33915; -.
DR SMR; O33915; -.
DR STRING; 266834.SMc02087; -.
DR PRIDE; O33915; -.
DR EnsemblBacteria; CAC46088; CAC46088; SMc02087.
DR GeneID; 61602972; -.
DR KEGG; sme:SMc02087; -.
DR PATRIC; fig|266834.11.peg.2930; -.
DR eggNOG; COG0372; Bacteria.
DR HOGENOM; CLU_025068_0_0_5; -.
DR OMA; TVGWCAQ; -.
DR UniPathway; UPA00223; UER00717.
DR Proteomes; UP000001976; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:InterPro.
DR GO; GO:0004108; F:citrate (Si)-synthase activity; IEA:InterPro.
DR GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-UniPathway.
DR CDD; cd06114; EcCS_like; 1.
DR Gene3D; 1.10.230.10; -; 1.
DR Gene3D; 1.10.580.10; -; 1.
DR InterPro; IPR016142; Citrate_synth-like_lrg_a-sub.
DR InterPro; IPR016143; Citrate_synth-like_sm_a-sub.
DR InterPro; IPR002020; Citrate_synthase.
DR InterPro; IPR019810; Citrate_synthase_AS.
DR InterPro; IPR024176; Citrate_synthase_bac-typ.
DR InterPro; IPR036969; Citrate_synthase_sf.
DR InterPro; IPR010953; Citrate_synthase_typ-I.
DR Pfam; PF00285; Citrate_synt; 1.
DR PIRSF; PIRSF001369; Citrate_synth; 1.
DR PRINTS; PR00143; CITRTSNTHASE.
DR SUPFAM; SSF48256; SSF48256; 1.
DR TIGRFAMs; TIGR01798; cit_synth_I; 1.
DR PROSITE; PS00480; CITRATE_SYNTHASE; 1.
PE 3: Inferred from homology;
KW Reference proteome; Transferase; Tricarboxylic acid cycle.
FT CHAIN 1..429
FT /note="Citrate synthase"
FT /id="PRO_0000169953"
FT ACT_SITE 306
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10117"
FT ACT_SITE 364
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10117"
FT CONFLICT 209
FT /note="E -> D (in Ref. 1; AAB82405)"
FT /evidence="ECO:0000305"
FT CONFLICT 229
FT /note="D -> N (in Ref. 1; AAB82405)"
FT /evidence="ECO:0000305"
FT CONFLICT 282
FT /note="V -> A (in Ref. 1; AAB82405)"
FT /evidence="ECO:0000305"
FT CONFLICT 290
FT /note="T -> A (in Ref. 1; AAB82405)"
FT /evidence="ECO:0000305"
FT CONFLICT 355
FT /note="I -> V (in Ref. 1; AAB82405)"
FT /evidence="ECO:0000305"
FT CONFLICT 427
FT /note="S -> T (in Ref. 1; AAB82405)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 429 AA; 48002 MW; F28D1C61427974F8 CRC64;
MSEKSATVTF GGKSADLPVR SGSIGPDVVD IGSLYKQTTM FTYDPGFTST ASCESKITYI
DGDEGVLLHR GFPIEQLAEH GDFLEVCYLL LYGELPTKAQ KADFDYRVTH HTMVHEQMSR
FFTGFRRDAH PMAVMCGCVG ALSAFYHDST DITDPHQRMV ASLRMIAKMP TIAAMAYKYH
IGQPFVYPKN DLDYASNFLR MCFAVPCEEY VVNPVLARAM DRIFILHADH EQNASTSTVR
LAGSSGANPF ACIAAGIACL WGPAHGGANE AALNMLAEIG TVDRIPEYIT KAKDKNDPFR
LMGFGHRVYK NYDPRAKIMQ KTTHEVLAEL GHKDDPLLEV AMELERIALT DEYFIEKKLY
PNIDFYSGIT LKALGFPTTM FTVLFALART VGWIAQWNEM IEDPEQRIGR PRQLYVGAPL
RDYVPISKR
